Acetylcholine (ACh) concentration-response curve for the aortic rings from male and female rats.

<p>Endothelium intact (Control) and 4-aminopyridine (4-AP 5 mM) curves (A); Difference of the area under curve (dAUC) control and 4-AP (B); Control and iberiotoxin (IbTX 30 nM) curves (C); dAUC control and IbTX (D); Control and apamin (0.5 µM) curves (E); dAUC control and apamin (F) and Control and charybdotoxin (ChTX 0.1 µM) curves (G); dAUC control and ChTX (H). <i>R_max</i> *P<0.05, male vs. female 4-AP, IbTX, Apamin and ChTX incubations. *P<0.05, dAUC male vs. female. Student’s <i>t</i>-test. Number of animals used is indicated in parentheses.</p

Parameters from the maximum response (<i>R_max</i>) and agonist concentration that produced 50% of the maximum response (<i>EC₅₀</i>) for the ACh concentration-response curve in aortic rings from male and female rats in an intact endothelium (Control) and incubated with tetraethylammonium (TEA), aminopyridine (4-AP), iberiotoxin (IbTX), charybdotoxin (ChTX) and apamin.

<p>Results are expressed as the mean ± SEM; maximal effect (<i>R_max</i>); -log one-half <i>R_max (pEC₅₀</i>); male and female intact endothelium (Control); tetraethylammonium (TEA); 4-aminopyridine(4-AP); iberiotoxin (IbTX); charybdotoxin (ChTX); apamin; and <i>N</i>^G-nitro-L-arginine methyl ester (L-NAME). *P<0.05 (<i>pEC₅₀</i> of female <i>vs.</i> male rats) and †P<0.05 (<i>R_max</i> of female <i>vs.</i> male rats). Results are expressed as the mean ± S.E.M. Differences were analyzed using Student’s <i>t</i>-test, and P<0.05 was considered significant.</p><p>Parameters from the maximum response (<i>R_max</i>) and agonist concentration that produced 50% of the maximum response (<i>EC₅₀</i>) for the ACh concentration-response curve in aortic rings from male and female rats in an intact endothelium (Control) and incubated with tetraethylammonium (TEA), aminopyridine (4-AP), iberiotoxin (IbTX), charybdotoxin (ChTX) and apamin.</p