Exploring Hydrologic Responses to Different Wildfire Spatial Patterns Through the Lens of Computational Modeling

Severe wildfire disturbances are becoming increasingly common in high-elevation forests of the western United States. These fires alter watershed hydrologic processes, threatening critical downstream water resources and aquatic ecosystems. However, watershed-scale postfire hydrologic responses and water balance changes are highly uncertain. While postfire effects on individual processes such as runoff, infiltration, evapotranspiration, and snow dynamics are relatively well known, the role of wildfire spatial patterns in governing hydrologic connectivity and interactions between water balance components is poorly understood due to challenges associated with measuring and comparing fires at large scales. This thesis aims to examine pattern-related postfire interactions between various hydrologic processes using computational modeling. Our goals are to identify the primary underlying relationships and to provide a methodological approach upon which a more comprehensive understanding of postfire watershed hydrology can be built. In Chapter 1, we briefly summarize the current knowledge base regarding postfire hydrology and introduce how hydrologic computational modeling has been used for postfire applications. Chapter 2, written as a manuscript, details the suite of modeling experiments used to explore the effects of wildfire spatial patterns on an idealized, snow-dominated mountain watershed. We used Neutral Landscape Model (NLM) algorithms to generate 150 fire mosaics with varying levels of aggregation and used a physically-based, distributed model to simulate each mosaic for a full water year. We found that each mosaic created a unique network of ow paths between the burned areas and the watershed outlet and that the size of the network controlled the timing of watershed discharge and soil water storage due to an infiltration capacity gradient between burned and unburned sites. Each fire mosaic generated the same amount of runoff from within the burned areas, but longer flow path networks resulted in more infiltration outside of the fire boundaries. However, because there was enough snow in the watershed to fully saturate the soil in every location, there was little difference in total annual discharge. While these results may be specific to snowmelt-dominated systems, they highlight the importance of considering the entire disturbance flow path network when evaluating watershed-scale postfire hydrologic responses

Storage hexamer utilization in Manduca sexta

In preparing for metamorphosis insects store in their hemolymph and fat bodies a major nutrient reserve of 500-kDa hexamerins. At least three hexamerins serve this function in Lepidoptera, including arylphorin (ArH) and two high methionine proteins (M-MtH and V-MtH). Six day-old adults of Manduca sexta Abbreviation: / ArH: arylphorin M-MtH: moderately high methionine hexamerin PBS: phosphate buffered saline V-MtH: very high methionine hexamerin Vg: vitellogeni

Storage hexamer utilization in two lepidopterans: differences correlated with the timing of egg formation

Most insects produce two or more storage hexamers whose constituents and developmental profiles are sufficiently different to suggest specialization in the ways that they support metamorphosis and reproduction. Hexamerin specializations are compared here in the Cecropia moth (Hyalophora cecropia), which produces eggs during the pupal-adult molt, and the Monarch butterfly (Danaus plexippus), which produces eggs under long-day conditions after adult eclosion. In both sexes of both species, reserves of arylphorin (ArH) were exhausted by the end of metamorphosis. In Cecropia, the same was true for the high-methionine hexamerins, V-MtH and M-MtH. But in short day Monarch females 20–30% of the pupal reserves of V-MtH and M-MtH survived metamorphosis, persisting until long-day conditions were imposed to stimulate egg formation. Differences in storage sites have been documented in other lepidopterans, with MtH reserves being found primarily in fat body protein granules and the ArH reserve being found primarily in the hemolymph. Similar differences could explain how a fraction of the MtH's, but not of ArH, escapes utilization during metamorphosis in a species with post-eclosion egg formation. No differences in utilization schedules were detected between V- and M-MtH, despite divergent compositions and antigenic reactivity

Parent reported sleep problems in preschool children with sickle cell anemia and controls in East London

Snoring and poor sleep may affect cognition, particularly in young children with chronic conditions. Parents of London preschoolers with sickle cell anemia (SCA; n = 22), matched controls (n = 24), and unselected typically developing (n = 142) preschoolers completed sleep questionnaires. Preschoolers with SCA had significantly more sleep problems when compared to matched controls and the larger population. Snoring occurred at least one to two nights a week for 79% of the SCA group. This is compared with 25% of matched controls and 33% of larger population. Randomized controlled trials to improve sleep in young children with SCA already at-risk for cognitive dysfunction should be considered

Haematopoietic stem cell health in sickle cell disease and its implications for stem cell therapies and secondary haematological disorders.

Gene modification of haematopoietic stem cells (HSCs) is a potentially curative approach to sickle cell disease (SCD) and offers hope for patients who are not eligible for allogeneic HSC transplantation. Current approaches require in vitro manipulation of healthy autologous HSC prior to their transplantation. However, the health and integrity of HSCs may be compromised by a variety of disease processes in SCD, and challenges have emerged in the clinical trials of gene therapy. There is also concern about increased susceptibility to haematological malignancies during long-term follow up of patients, and this raises questions about genomic stability in the stem cell compartment. In this review, we evaluate the evidence for HSC deficits in SCD and then discuss their potential causation. Finally, we suggest several questions which need to be addressed in order to progress with successful HSC manipulation for gene therapy in SCD

Executive performance on the preschool executive task assessment in children with sickle cell anemia and matched controls

Executive deficits are commonly reported in children with sickle cell anemia. Earlier identification of executive deficits would give more scope for intervention, but this cognitive domain has not been routinely investigated due to a lack of age-appropriate tasks normed for preschool children. In particular, information relating to patient performance on an executive task that reflects an everyday activity in the classroom could provide important insight and practical recommendations for the classroom teacher at this key developmental juncture as they enter the academic domain. The performance of 22 children with sickle cell anemia was compared to 24 matched control children on the Preschool Executive Task Assessment. Findings reveal that children with sickle cell anemia are performing poorer than their matched peers on this multi-step assessment. In particular, children with sickle cell anemia required more structured support to shift focus after a completed step, as reflected by poorer scores in the quantitative Sequencing and Completion domains. They also required more support to stay on task, as seen by poorer ratings in the qualitative Distractibility domain. ABBREVIATIONS: PETA: Preschool Executive Task Assessment; SCA: Sickle Cell Anemia; EF: Executive Functioning