Recombinant Dna Vector, Method For Producing Plants That Are Resistant To Environmental Stresses, And Uses Thereof

As plantas são influenciadas por umu grande número de fatores ambientais bióticos e abióticos e recorrentemente estresses abióticos silo mais graves, abrando todas as funções da planta, resultando em redução do crescimento e da produtividade. Nesse sentido, a identificação e a compressão dos mecanismos de tolerância abiáticos são fundamentais no desenvolvimento de novas cultivares tolerantes á seca. Dessa forma, a presente invenção proporciona teu método de produção de plantas que contém em suas células uma sequência de nucleotídeos de cana-de-açúcar e a superexpressão desse gene leva a planta em questão à maior tolerância a estresses abióticos. De uma forma mais ampla, descreve-se um polinucleotídeo que codifica a proteína de cana-de-açúcar, o qual é expresso por um promotor e um treinador que funcionam em plantas. Verifica-se que este gene confere tolerância a diferentes estresses abióticos.Plants are influenced by a large number of biotic and abiotic environmental factors, recurrent abiotic stresses being the most serious and affecting all organic, functions of the plant, leading to reduced growth and productivity. In this context, identifying and understanding abiotic resistance mechanisms are crucial for developing new drought-resistant are cultivars. The present invention thus provides an method for producing plants containing in their calls a sugarcane nucleotide sequence, overexpression of this gene increasing the paint’s resistance to abiotic stresses. More generally, a polynucleotide is described that encodes sugarcane protean and is expressed by a promoter and a terminator that are operational in plants. This gene been discovered to impart resistance to various abiotic stresses.WO2012083393 (A2); WO2012083393 (A3)C12N5/04WO2011BR00201C12N5/04US2007292874 (A1

Method For Producing Plants That Are Resistant To Environmental Stresses, Uses Thereof And Recombinant Dna Vector

As plantas são influenciadas por um grande número de fatores ambientais bióticos e abióticos e recorrentemente estresses abióticos são mais graves, afetando todas as funções da planta, resultando em redução do crescimento e da produtividade. Nesse sentido, a identificação e a compreensão dos mecanismos de tolerância antibióticos são fundamentais no desenvolvimento de novas cultivares tolerantes à seca. Dessa forma, a presente invenção proporciona uni método de produção de plantas que contém em suas células uma sequência de nuclectideos de cana-de-açúcar e a superexpressão desse gene leva a planta em questão à maior tolerância a estresses abióticos, de uma forma mais ampla, descreve-se uni polimicleotideo que codifica a proteína de cana-de-açúcar, o qual é expresso por um promotor e um terminador que funcionam em plantas. Verifica-se que este gene confere tolerãncia a diferentes estresses antibioticos.Plants are influenced by n large number of biotic and biotic environmental factors, recurrent abiotic stresses being the most serious and affecting all organic functions of the plants, leading to reduced growth and productivity. In this context, identifying and understanding abiotic resistance mechanisms are crucial for developing new drought-resistant cultivars. The present invention thus provides a method for producing plants containing in their cells a sugarcane nucleotide sequence, overexpression of this gene increasing the plant's resistance to abiotic stresses. More generally, a polynucleotide is described that encodes sugarcane protein and is expressed by a promoter and a terminator that are operational in plants. This gene has been discovered to impart resistance to various abiotic stresses.WO2012083394 (A3); WO2012083394 (A2)C12N15/04WO2011BR00202C12N15/04US7834146 (B2

Genes Associated To Sucrose Content

Modern sugarcane cultivars are complex hybrids resulting from crosses among several species of the Saccharum genus. Traditional breeding methods have been extensively employed in different countries along the past decades to develop varieties with increased sucrose yield, and resistant to plagues and diseases. Conventional varietal improvement is, however, limited by the narrow pool of suitable markers. In this sense, molecular genetics is seen as a promising tool to assist in the process of molecular marker identification. The present invention concerns the identification of 348 genes associated with sucrose content in sugarcane plants. The genes were found to be differentially expressed when high sucrose and low sucrose plants and populations of plants were compared and/or when high and low sucrose internodes were compared. The expression data was obtained using cDNA microarray and quantitative PCR technologies. The genes identified can be used to identify, distinguish, characterize and/or develop plants with increased sucrose content. More preferably SEQ ID Nos:. 1 to 203 should be useful as molecular markers. SEQ ID Nos: 204 to 228 are given as controls or examples of genes never associated with sucrose content. SEQ ID Nos. 1-203 and SEQ ID Nos. 229 to 373 can be targeted in the development of transgenic or non-transgenic varieties with increased sucrose content.US2010162442 (A1)A01H5/00US20100713112A01H5/0

Genes Associated To Sucrose Content

Modern sugarcane cultivars are complex hybrids resulting from crosses among several species of the Saccharum genus. Traditional breeding methods have been extensively employed in different countries along the past decades to develop varieties with increased sucrose yield, and resistant to plagues and diseases. Conventional varietal improvement is, however, limited by the narrow pool of suitable markers. In this sense, molecular genetics is seen as a promising tool to assist in the process of molecular marker identification. The present invention concerns the identification of 348 genes associated with sucrose content in sugarcane plants. The genes were found to be differentially expressed when high sucrose and low sucrose plants and populations of plants were compared and/or when high and low sucrose internodes were compared. The expression data was obtained using cDNA microarray and quantitative PCR technologies. The genes identified can be used to identify, distinguish, characterize and/or develop plants with increased sucrose content. More preferably SEQ ID Nos: 1 to 203 should be useful as molecular markers SEQ ID Nos: 204 to 228 are given as controls or examples of genes never associated with sucrose content. SEQ ID Nos. 1-203 and SEQ ID Nos. 229 to 373 can be targeted in the development of transgenic or non-transgenic varieties with increased sucrose content.US2012079621 (A1)A01H5/00A01H1/06A01H5/10US201113070392A01H5/00A01H1/06A01H5/1