Parashewanella tropica sp. nov., a Novel Bacterium Isolated from a Marine Sponge from Chuuk lagoon, Federated States of Micronesia

A mesophilic, straight rod-shaped, non-flagellated bacterium, designated MEBiC05444T, was isolated from a marine sponge collected from Chuuk lagoon, Federated States of Micronesia. The strain was Gram-negative, catalase- and oxidase-positive and facultative anaerobe. The isolate aerobically grew at 8-38°C (optimum range 24-32°C), pH 4.01

Luteithermobacter Park & Namirimu & Yang & Kwon 2020, GEN. NOV.

DESCRIPTION OF LUTEITHERMOBACTER GEN. NOV. Luteithermobacter (Lu.te.i.ther.mo.bac’ter. L. masc. n. luteus yellowish; Gr. adj. thermos hot; N.L. masc. n. bacter a rod; N.L. masc. n. Luteithermobacter a rod-shaped yellowish thermophile). Includes Gram-stain-negative, neutrophilic and thermophilic marine bacteria that grow under aerobic conditions. Cells are rod-shaped and form pale yellow colonies. The cell wall comprises fatty acids such as saturated forms with carbon number 14–16, unsaturated forms with carbon number 16–18, hydroxyl form with carbon number 14, and cyclic form with carbon number 19. The only respiratory quinone is Q-10, and the DNA G+C content is approximately 55 mol%. The type species is Luteithermobacter gelatinilyticus.Published as part of Park, Mi-Jeong, Namirimu, Teddy, Yang, Sung-Hyun & Kwon, Kae Kyoung, 2020, Description of Luteithermobacter gelatinilyticus gen. nov., sp. nov., and Paremcibacter congregatus gen. nov., comb. nov. via reclassification of the genus Emcibacter, pp. 4691-4697 in International Journal of Systematic and Evolutionary Microbiology 70 (8) on page 4695, DOI: 10.1099/ijsem.0.004334, http://zenodo.org/record/622400

Paremcibacter congregatus Park, Namirimu, Yang & Kwon, 2020, COMB. NOV.

DESCRIPTION OF PAREMCIBACTER CONGREGATUS COMB. NOV. Paremcibacter congregatus comb. nov. (con.gre.ga′ tus. L. part. adj. congregatus clustered, always clustered when cultured in lipid medium). Basonym: Emcibacter congregatus Zhao et al. 2018. The description is as given by Zhao et al. 2018. The type strain, ZYLT (=KCTC 62328 T =JCM 32378 T =MCCC 1K03526 T), was isolated from a cultured in situ sediment sample collected on the coast of the East China Sea. The GenBank/EMBL/MMBJ accession number for the 16S rRNA gene sequence is MG051279 and the genome sequence is CP041025.Published as part of Park, Mi-Jeong, Namirimu, Teddy, Yang, Sung-Hyun & Kwon, Kae Kyoung, 2020, Description of Luteithermobacter gelatinilyticus gen. nov., sp. nov., and Paremcibacter congregatus gen. nov., comb. nov. via reclassification of the genus Emcibacter, pp. 4691-4697 in International Journal of Systematic and Evolutionary Microbiology 70 (8) on page 4696, DOI: 10.1099/ijsem.0.004334, http://zenodo.org/record/622400

Description of Luteithermobacter gelatinilyticus gen. nov., sp. nov., and Paremcibacter congregatus gen. nov., comb. nov. via reclassification of the genus Emcibacter

Strain MEBiC09520T, which was isolated from a tidal sediment in Incheon, Korea, is a pale yellow, rod-shaped bacterium, cells of which are 0.4–0.5 µm in width and 1.5–2 µm in length. Strain MEBiC09520T shared 95.17 and 92.57% 16S rRNA gene sequence similarity with Emcibacter nanhaiensis and E. congregatus , respectively. It grew optimally at pH 6.0, at 55 °C and with 2.5–3.5% (w/v) NaCl. Its polar lipid components included phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), an unidentified phospholipid (PL), three unidentified aminolipids (ALs) and two unidentified lipids (L). The fatty acids C16:0, C19:0 cyclo ω8c, C14:0 2-OH and summed feature 8 (C18:1ω7c and/or C18:1ω6c) were predominantly present in its cell wall. Strain MEBiC09520T was thermophilic, while E. nanhaiensis and E. congregatus were mesophilic. Although E. nanhaiensis showed no nitrate reduction activity, MEBiC09520T and E. congregatus showed a positive reaction. These strains differed in carbohydrate utilization. In particular, E. congregatus was able to thrive on various carbohydrate substrates as compared to the other strains. The average nucleotide identity value was 69.92% between strain MEBiC09520T and E. congregatus ZYLT, 70.38% between E. congregatus ZYLT and E. nanhaiensis HTCJW17T, and 72.83% between strain MEBiC09520 and E. nanhaiensis HTCJW17T. Considering these differences, strain MEBiC09520T (=KCCM 43320T=MCCC 1K03920T) is suggested to represent and novel species of a new genus, Luteithermobacter gelatinilyticus gen. nov., sp. nov., and E. congregatus should be reclassified as Paremcibacter congregatus gen. nov., comb. nov.</jats:p

Paremcibacter Park & Namirimu & Yang & Kwon 2020, GEN. NOV.

DESCRIPTION OF PAREMCIBACTER GEN. NOV. Paremcibacter (Par.em.ci.bac’ter. Gr. prep. para beside; N.L. masc. n. Emcibacter a genus name; N.L. masc. n. Paremcibacter resembling the genus Emcibacter). Comprises Gram-negative, neutrophilic and mesophilic marine bacteria that grow under aerobic conditions. Cells are rod-shaped with sticky filaments and form pale yellow colonies. The cell wall mainly consists of fatty acids such as saturated forms with carbon number 14–16, unsaturated forms with carbon number 16–18 and hydroxyl form with carbon number 14. The sole respiratory quinone is Q-10. The DNA G+C content is around 52 mol%. The type species is Paremcibacter congregatus.Published as part of Park, Mi-Jeong, Namirimu, Teddy, Yang, Sung-Hyun & Kwon, Kae Kyoung, 2020, Description of Luteithermobacter gelatinilyticus gen. nov., sp. nov., and Paremcibacter congregatus gen. nov., comb. nov. via reclassification of the genus Emcibacter, pp. 4691-4697 in International Journal of Systematic and Evolutionary Microbiology 70 (8) on page 4696, DOI: 10.1099/ijsem.0.004334, http://zenodo.org/record/622400

Microbial Community Structure and Functional Potential of Deep-Sea Sediments on Low Activity Hydrothermal Area in the Central Indian Ridge

Little is known about the community structure and metabolic potential of microbial communities in hydrothermal fields in the Central Indian Ridge (CIR). In this study, a metagenomic sequencing approach was conducted to explore the microbial diversity in three sediment samples collected during the 2019 expedition from two recently discovered hydrothermal vent fields; Invent E and Onnuri Vent Field (OVF). Analysis of unassembled metagenomic reads using the Metagenomic analysis server (MG-RAST) revealed that microbial communities of the two sampling sites were very similar, showing the dominance of Bacteria over Archaea. Proteobacteria, Firmicutes, Bacteroidetes, as well as Euryarchaeota were dominant in all samples. Functional annotation based on KEGG categories shows that the microbial populations in these vent fields possess metabolic capabilities for aerobic respiration, carbon fixation through the Calvin–Bassham–Benson (CBB) cycle, the reverse tricarboxylic acid (rTCA) cycle, and reductive acetyl-CoA pathway as well as sulfur and nitrogen metabolisms. Comparative metagenome analysis with different datasets obtained from different ocean ridges showed that microbial communities at low activity or hydrothermally influenced area differ from highly active hydrothermal communities. This study provides insights into the genetic diversity and functional capability of the microbial communities of slow to intermediate spreading hydrothermal systems.</jats:p

Alkalibacter rhizosphaerae sp. nov., a CO-utilizing bacterium isolated from tidal flat sediment, and emended description of the genus Alkalibacter

A novel anaerobic, rod-shaped, non-motile bacterium, designated strain ES005T, was isolated from tidal flat sediments near the rhizosphere of Phragmites australis at Eulsukdo Island, Republic of Korea. A polyphasic approach revealed that cells of the strain were Gram-stain-positive, catalase-and oxidase-negative, non-spore-forming rods. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain ES005T belonged to the family Eubacteriaceae, class Clostridia and showed the highest sequence similarity to Alkalibacter mobilis (97.52%) and followed by Alkalibacter saccharofermentans Z-79820T (96.72%). The OrthoANI value between strain ES005T and A. mobilis was 69.67%. Strain ES005T grew optimally at 33–37 °C, at pH 6.0–7.0 and in the presence of 1–2% (w/v) NaCl. Growth in 12.5% CO atmosphere was observed. Acetate and formate were end products of fructose fermentation and growth on CO. The major cellular fatty acids of strain ES005T were C14:0 (39.1%) and C16:0 (26.6%). The major polar lipids were diphoshatidylgycerol, phosphatidylglycerol and three unidentified phospholipids. The DNA G+C content of strain ES005T was 46.9mol%. Based on the phenotypic, phylogenetic, genomic and chemotaxonomic features of the isloate, strain ES005T represents a novel species, for which the name Alkalibacter rhizosphaerae sp. nov. is proposed. The type strain is ES005T (=KCTC 25246T=JCM 34530T). © 2022 The Authors.11Nsciescopu