Central role for the XRCC1 BRCT I domain in mammalian DNA single-strand break repair

The DNA single-strand break repair (SSBR) protein XRCC1 is required for genetic stability and for embryonic viability. XRCC1 possesses two BRCA1 carboxyl-terminal (BRCT) protein interaction domains, denoted BRCT I and II. BRCT II is required for SSBR during G1 but is dispensable for this process during S/G2 and consequently for cell survival following DNA alkylation. Little is known about BRCT I, but this domain has attracted considerable interest because it is the site of a genetic polymorphism that epidemiological studies have associated with altered cancer risk. We report that the BRCT I domain comprises the evolutionarily conserved core of XRCC1 and that this domain is required for efficient SSBR during both G1 and S/G2 cell cycle phases and for cell survival following treatment with methyl methanesulfonate. However, the naturally occurring human polymorphism in BRCT I supported XRCC1-dependent SSBR and cell survival after DNA alkylation equally well. We conclude that while the BRCT I domain is critical for XRCC1 to maintain genetic integrity and cell survival, the polymorphism does not impact significantly on this function and therefore is unlikely to impact significantly on susceptibility to cancer

What Man Would Put Up With Me... Eartha White Carries Out the Prophecy of Her Name

New clipping about Eartha Mary Magdalene White. No date given

Observing the CMB at High-l using the VSA and AMI

We discuss two experiments - the Very Small Array (VSA) and the Arcminute MicroKelvin Imager (AMI) - and their prospects for observing the CMB at high angular multipoles. Whilst the VSA is primarily designed to observe primary anisotropies in the CMB, AMI is designed to image secondary anisotropies via the Sunyaev-Zel'dovich effect. The combined l-range of these two instruments is between l = 150 and ~10000.Comment: 8 pages, 13 figures. To be published in the proceedings of "The Cosmic Microwave Background and its Polarization", New Astronomy Reviews, (eds. S. Hanany and K.A. Olive