Effect of phyto-components of Allium sativum and Syzyginum aromaticum on the performance and biochemical indices in grower broiler chickens

The potentials of Allium sativum and Syzyginum aromaticum on the performance and immunocompetence of grower broiler chickens was focused in this study. A total of one hundred and five (105) day old broiler chicks(Anak breed) were used in the study. Broiler starter diet was used to managed the birds until they attained five weeks of age. Thereafter, they were randomly allotted to seven treatments of 15 birds of three replicate each. To the treatments T1- T5: 0, 5, 10, 15, 20 g Kg-1 garlic-clove blend was supplemented, while 5g Kg-1 garlic alone (T6) and 2g Kg-1 clove alone (T7) were supplemented to the diets. Water, and supplemented feed were offered ad libitum. The duration of the experiment lasted to five weeks. Phytochemical analysis of the two spices revealed the presence of Alkaloids, flavonoids, saponins, steroids, phenols, terpenoids, anthraquinones and tannins, while, quantitative phytochemical screening showed high amount of Alkaloids (7.20 ± 0.05mg/100g), tannins (4.80 ± 0.03mg/100g), saponins (4.30 ± 0.02 mg/100g), flavonoids (2.18 ± 0.03), and anthraquinones (1.40 ± 0.03 mg/100g). The proximate analysis of Syzyginum aromaticum also revealed high amount of Carbohydrates (oligosaccharides) (52.2 ± 0.02), fibre (20.00 ± 0.1), fats (12.1 ± 0.45) and ash (5.2 ± 0.01). The Feed Conversion Ratio (FCR) in all the treatments was comparable to the control and better in T2, T5, T6 and T7. There was no significant difference (P ≤ 0.05) in the serum Blood Urea Nitrogen (BUN) and serum Glutamate Oxalate Transaminase (SGOT) between the treatments and the control group. The serum total protein and the serum albumin in the treated groups were not significantly different ((P≤ 0.05) while the serum globulin differ (P≤ 0.05) between the treatments and the control groups. The performance characteristics and serum biochemical indices displayed by the experimental birds clearly showed the potency of these spices in improving the immuno-competence of the experimental birds

In vivo Trypanostatic Activity of Tephrosia linearis Extract on Trypanosoma evansi

In vivo antitrypanosomal activity of 70% methanol extract of Tephrosia linearis was evaluated. Mice infected with Trypanosoma evansi in different groups were administered 100, 200, 300 and 400 mg/kg body weight/day of the seventy percent (v/v) (methanol/water) crude extract of T. linearis intraperitoneally. The positive control group was treated with standard drug, berenil, while the negative control was infected and treated with normal saline. Doses of 300 and 400 mg/kg bw i.p. per day were found to significantly reduce the parasite loads and sustained the animals in the respective groups up to 3 and 4 weeks beyond the experimental period. There is drop in the percentage PCV during the first six days of the treatment but this was reversed in the subsequent days, except in the negative control group. The animals administered the highest effective (trypanostatic) dose of 400 mg/kg body weight for five consecutive days prior to infection were observed to develop infection 72 h post inoculation. The LD50 determined was found to be 2800 mg/kg bw. It is obvious that, higher doses of 300 and 400 mg/kg bw has trypanostatic effect. Though, the extract could not show any prophylactic activity, further purification may possibly yield a fraction with trypanocidal effect. Keywords: Tephrosia linearis, Methanol extract, Trypanosoma evansi, Antitrypanosomal activity, In viv

Apigetrin-enriched Pulmeria alba extract prevents assault of STZ on pancreatic β-cells and neuronal oxidative stress with concomitant attenuation of tissue damage and suppression of inflammation in the brain of diabetic rats

In the present study, in vitro, in vivo, and in silico models were used to evaluate the therapeutic potential of Pulmeria alba methanolic (PAm) extract, and we identified the major phytocompound, apigetrin. Our in vitro studies revealed dose-dependent increased glucose uptake and inhibition of α-amylase (50% inhibitory concentration (IC50)= 217.19 µg/mL), antioxidant (DPPH, ferric-reducing activity of plasma (FRAP), and lipid peroxidation (LPO) [IC50 = 103.23, 58.72, and 114.16 µg/mL respectively]), and anti-inflammatory potential (stabilizes human red blood cell (HRBC) membranes, and inhibits proteinase and protein denaturation [IC50 = 143.73, 131.63, and 198.57 µg/mL]) by the PAm extract. In an in vivo model, PAm treatment reversed hyperglycemia and attenuated insulin deficiency in rats with streptozotocin (STZ)-induced diabetes. A post-treatment tissue analysis revealed that PAm attenuated neuronal oxidative stress, neuronal inflammation, and neuro-cognitive deficiencies. This was evidenced by increased levels of antioxidants enzymes (superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH)), and decreased malondialdehyde (MDA), proinflammatory markers (cyclooxygenase 2 (COX2), nuclear factor (NF)-κB and nitric oxide (NOx)), and acetylcholinesterase (AChE) activities in the brain of PAm-treated rats compared to the STZ-induced diabetic controls. However, no treatment-related changes were observed in levels of neurotransmitters, including serotonin and dopamine. Furthermore, STZ-induced dyslipidemia and alterations in serum biochemical markers of hepatorenal dysfunction were also reversed by PAm treatment. Extract characterization identified apigetrin (retention time: 21,227 s, 30.48%, m/z: 433.15) as the major bioactive compound in the PAm extract. Consequently, we provide in silico insights into the potential of apigetrin to target AChE/COX-2/NOX/NF-κB Altogether the present study provides preclinical evidence of the therapeutic potential of the apigetrin-enriched PAm extract for treating oxidative stress and neuro-inflammation associated with diabetes