17 research outputs found
Suppression of Adiponectin by Aberrantly Glycosylated IgA1 in Glomerular Mesangial Cells In Vitro and In Vivo
<div><p>The pathogenesis of IgA nephropathy (IgAN) may be associated with the mesangial deposition of aberrantly glycosylated IgA1. To identify mediators affected by aberrantly glycosylated IgA1 in cultured human mesangial cells (HMCs), we generated enzymatically modified desialylated and degalactosylated (deSial/deGal) IgA1. The state of deglycosylated IgA1 was confirmed by lectin binding to <em>Helix aspersa</em> (HAA) and <em>Sambucus nigra</em> (SNA). In the cytokine array analysis, 52 proteins were upregulated and 34 were downregulated in HMCs after stimulation with deSial/deGal IgA1. Among them, the secretion of adiponectin was suppressed in HMCs after stimulation with deSial/deGal IgA1. HMCs expressed mRNAs for adiponectin and its type 1 receptor, but not the type 2 receptor. Moreover, we revealed a downregulation of adiponectin expression in the glomeruli of renal biopsy specimens from patients with IgAN compared to those with lupus nephritis. We also demonstrated that aberrantly glycosylated IgA1 was deposited in the mesangium of patients with IgAN by dual staining of HAA and IgA. Moreover, the urinary HAA/SNA ratio of lectin binding was significantly higher in IgAN compared to other kidney diseases. Since adiponectin has anti-inflammatory effects, including the inhibition of adhesion molecules and cytokines, these data suggest that the local suppression of this adipokine by aberrantly glycosylated IgA1 could be involved in the regulation of glomerular inflammation and sclerosis in IgAN.</p> </div
The expression of adiponectin, AdipoR1 and AdipoR2 genes in cultured human mesangial cells (HMCs).
<p>(A) The cDNA from human adipocytes was utilized as a positive control for adiponectin and the cDNA from human hepatocytes was used as a control for AdipoR1 and AdipoR2. RT-PCR for adiponectin in HMCs after stimulation with either native or deSial/deGal IgA1 afforded cDNA bands of the same size (256 bp) as that amplified from human adipocyte cDNA. RT-PCR for AdipoR1 in HMCs afforded cDNA bands of the same size (70 bp) as that amplified from human hepatocytes. The HMCs did not express AdipoR2 mRNA. The expression of the GAPDH gene was used as an internal standard (350 bp). (B) The densitometric analysis of the expression of each cDNA.</p
The ELISA of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1.
<p>In HMCs, native IgA upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 Β΅g/ml) for 48 h. For the time course study, HMCs were cultured with 25 Β΅g/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means Β± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. *<i>P</i>β=β0.05 vs. medium control; #<i>P</i>β=β0.01, native IgA vs. deSial/deGal IgA1.</p
The profiles of the patients included in the analysis of urine samples.
<p>OKD, other kidney disease; IgAN, IgA nephropathy; eGFR, estimated glomerular filtration rate; RBC, red blood cell; HAA, <i>Helix aspersa</i>; SNA, <i>Sambucus nigra agglutinin</i>.</p>a<p>: <i>P</i><0.01,</p>b<p>: <i>P</i><0.05.</p
Cytokine array analysis after stimulation of cultured human mesangial cells (HMCs) with native IgA or deSial/deGal IgA1.
<p>The HMCs were stimulated with native (A) or deSial/deGal IgA1 (50 Β΅g/ml) (B) for 48 h. The culture supernatants were then applied for a protein array analysis. After incubation of samples with array membranes for 2 h at room temperature, the spots on the membranes were scanned and digitized. The signal intensities of the spots obtained from two separate experiments were analyzed. (The proteins that were up- or downregulated by approximately 2 fold are summarized in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033965#pone-0033965-t001" target="_blank">Tables 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033965#pone-0033965-t002" target="_blank">2</a>.) The spots shown by arrows correspond to adiponectin. The intensity of the spots in the membrane stimulated with native IgA was higher than that of cells stimulated with deSial/deGal IgA1 in HMC (A, B).</p
The expression of adiponectin, Ξ±SMA and vWF in human renal biopsies.
<p>Immunofluorescent staining of renal biopsy specimens from patients with minor glomerular abnormalities (MGA; A, D, G, J, M and P), lupus nephritis (LN; B, E, H, K, N and Q) and IgA nephropathy (IgAN; C, F, I, L, O and R) are shown. (Upper panels) Each section was stained for Ξ±SMA (a marker of activated mesangial cells, red) (AβC) and adiponectin (green) (DβF). Some Ξ±SMA-positive cells were colocalized with adiponectin-positive cells (yellow) (GβI). Double positive areas were predominant in the glomeruli of LN patients (H, arrows) as compared to IgAN patients (I, arrows). (Lower panels) Each section was stained for vWF (a marker of endothelial cells, red) (JβL) and adiponectin (green) (MβO). Some vWF-positive cells were colocalized with adiponectin-positive cells (yellow) (PβR, arrowheads). Double positive areas predominated in the glomeruli of MGA patients (P). Strong and segmental staining of adiponectin was recognized in the glomeruli of LN patients (E and N). The adiponectin staining in the glomeruli of IgAN patients was weaker than that of LN and MGA patients. Scale bars represent 100 Β΅m.</p
Double staining of HAA lectin and IgA in renal biopsy specimens, and the quantitative analysis of staining.
<p>Renal biopsy specimens from patients with minor glomerular abnormalities (MGA) (A, D and G), lupus nephritis (LN) (B, E and H) and IgA nephropathy (IgAN) (C, F and I) were stained for HAA lectin (red) (AβC), IgA (green) (DβF) and their images were merged (yellow) (GβI). In IgAN patients, the IgA-positive areas were colocalized with HAA lectin-positive area in the glomeruli. On the other hand, there were IgA-positive areas but no HAA lectin-positive areas in the glomeruli of LN patients. The relative double positive intensities in the glomeruli of IgAN patients were increased compared to those of LN or MGA patients (J). The double positive areas in the glomeruli of IgAN patients were increased compared to those with LN or MGA (K). Note that certain segments of tubules and Bowman's capsules were also stained with HAA lectin. Each column consists of the means Β± SE. MGA, <i>n</i>β=β9; LN, <i>n</i>β=β14; and IgAN, <i>n</i>β=β17. **<i>P</i><0.01. The scale bars represent 100 Β΅m.</p
The adiponectin staining score in the glomeruli of renal biopsy specimens and the relationship between the adiponectin staining score and serum IgA in patients with IgA nephropathy (IgAN).
<p>(A) The adiponectin staining score in IgAN was significantly decreased in IgAN patients compared with that in patients with lupus nephritis (LN). Each column shown the means Β± SE. MGA, <i>n</i>β=β9; MCD, <i>n</i>β=β8; LN, <i>n</i>β=β17; and IgAN, <i>n</i>β=β19. **<i>P</i><0.01. (B) A significantly negative correlation was found between the adiponectin staining score and serum IgA in IgAN patients (<i>n</i>β=β19) (rβ=ββ0.488, <i>P</i>β=β0.034).</p
The urinary HAA/SNA binding ratio in patients with IgAN and other kidney diseases.
<p>The HAA/SNA ratios were determined by the HAA or SNA lectin binding assays using anti-IgA antibody-coated plates. The HAA/SNA ratio was higher in IgAN patients compared to patients with other kidney diseases (OKD) (<i>P</i><0.05) (A). The level of HAA binding corrected for the urinary creatinine concentration was also higher in IgAN patients compared to patients with OKD (<i>P</i><0.05) (B). Each column consists of the means Β± SE. OKD, <i>n</i>β=β142; IgAN, <i>n</i>β=β78 *<i>P</i><0.05.</p
Multivariate odds ratio for ankle-brachial pulse wave velocity (baPWV) among patients with CKD displayed as the odds ratio (OR) (solid boxes) with 95% confidence intervals (CIs) (horizontal limit lines).
<p>For continuous variables, the unit of change is given in parenthesis based on the multivariate model described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056695#pone-0056695-t002" target="_blank">Table 2</a>. MBP, mean blood pressure; eGFR, estimated glomerular filtration rate; PTH, parathyroid hormone; 1,25D, 1,25-dihydroxyvitamin D; FGF23, fibroblast growth factor 23.</p