Additional file 2: Table S2. of Dissemination of clonal complex 2 Acinetobacter baumannii strains co-producing carbapenemases and 16S rRNA methylase ArmA in Vietnam

MLST analysis on PubMLST scheme in 93 A. baumannii isolates. (XLSX 14 kb

Additional file 1: Table S1. of Dissemination of clonal complex 2 Acinetobacter baumannii strains co-producing carbapenemases and 16S rRNA methylase ArmA in Vietnam

Assembly summary report of 93 A. baumannii isolates using CLC Genomics Workbench version 5.5. (XLSX 15 kb

MICs of β-lactams for <i>P</i>. <i>aeruginosa</i> NCGM1984 and <i>E</i>. <i>coli</i> transformants with <i>bla</i>_IMP-1 and <i>bla</i>_IMP-34.

<p>MICs of β-lactams for <i>P</i>. <i>aeruginosa</i> NCGM1984 and <i>E</i>. <i>coli</i> transformants with <i>bla</i>_IMP-1 and <i>bla</i>_IMP-34.</p

Kinetic parameters of the β-lactamases IMP-1 and IMP-34 with various substrates.

<p>Kinetic parameters of the β-lactamases IMP-1 and IMP-34 with various substrates.</p

Genomic islands containing class 1 integrons in <i>Pseudomonas aeruginosa</i> NCGM1984.

<p>(A) Genomic island containing integrons A and B. The genomic island was inserted between NCGM1984_2402 (PA2752 in the complete genome of <i>P</i>. <i>aeruginosa</i> PAO1: accession no.AE004091) and NCGM1984_2462 (PA2749). (B) Genomic island containing integron C. The genomic island was inserted between NCGM1984_4147 (PA1181) and NCGM1984_4144 (PA1179). (C) The genomic structure of integrons A and C. The structure of integron A was identical to that of integron C. These integrons contained <i>bla</i>_IMP-34. The red arrow indicates <i>bla</i>_IMP-34. Blue arrows indicate IRi and IRt. Green arrows indicate direct repeats. (D) Genomic structure of integron B. Blue arrows indicate IRi and IRt. Green arrows indicate direct repeats.</p

Image_1_Isolation and identification of Wickerhamiella tropicalis from blood culture by MALDI-MS.pdf

Wickerhamiella is a genus of budding yeast that is mainly isolated from environmental samples, and 40 species have been detected. The yeast isolated from human clinical samples usually only contain three species: W. infanticola, W. pararugosa and W. sorbophila. In this study, we isolated W. tropicalis from a blood sample of a six-year-old female with a history of B-cell precursor lymphoblastic leukemia in Japan in 2022. Though the strain was morphologically identified as Candida species by routine microbiological examinations, it was subsequently identified as W. tropicalis by sequencing the internal transcribed spacer (ITS) of ribosomal DNA (rDNA). The isolate had amino acid substitutions in ERG11 and FKS1 associated with azole and echinocandin resistance, respectively, in Candida species and showed intermediate-resistant to fluconazole and micafungin. The patient was successfully treated with micafungin. Furthermore, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) detected three novel peaks that are specific for W. tropicalis, indicating that MALDI-MS analysis is useful for rapid detection of Wickerhamiella species in routine microbiological examinations.</p