12 research outputs found
The Reactions of 2-(Trimethylsilyl)ethyl Benzenesulfenate as an Equivalent of Sulfenate Anion or a Precursor of Sulfenyl Cation (Commemoration Issue Dedicated to Professor Shigeo Tanimoto On the Occation of His Retirement)
Get PDFRecent Progress in the Chemistry of 1, 3-Dithiolanes and Related Compounds (Commemoration Issue Dedicated to Professor Masaya Okano on the Occasion of his Retirement)
Get PDFThe Diels-Alder Reaction of N, N-Diethyl-1, 3-butadienylamine with Several (β-Substituted Styrenes and Use of the Reaction Products for the One-Pot Synthesis of 2-Substituted Biphenyls
Get PDFThe Reaction of 2, 2'-[l, 2-Ethanediylbis (thio)] bis-1, 3-dithiolane with Carbonyl Compounds
Get PDFDiels-Alder Addition of N,N-Diethyl-1,3-butadienylamine to Dehydroaromatic Intermediates Generated from Some Haloaromatics (Commemoration Issue Dedicated to Professor Tatsuo Yamamoto on the Occasion of his Retirement)
Get PDFLiver-type of tissue non-specific alkaline phosphatase is induced during mouse bone and tooth cell differentiation
Get PDFBackground and objective: Tissue non-specific alkaline phosphatase (TNSALP) contains two types?bone- and liver-type?which are produced from the same gene due to differences in splicing. These two differ in their promoter, but the amino acid sequences of the mature proteins are identical. In this study, we examined the relationship between the two types of TNSALP expression and osteoblast differentiation. Design: Gene expression of the two types of TNSALP was observed by reverse transcription-polymerase chain reaction. MC3T3-NM4 was sub-cloned from an established mouse osteoblastic cell line in which osteoblast characters do not appear without dexamethasone. The C2C12 mouse myoblastic cell line, which can be induced to osteoblasts with bone morphogenic protein 2, and organ-cultured tooth germs were also used in this work. Results: The gene expression of liver-type TNSALP was observed in only MC3T3-NM4 activated by dexamethasone. For C2C12, the gene expression of bone-type TNSALP was observed even in non-induced conditions where myotubes were formed, whereas the liver-type TNSALP mRNA was only expressed when C2C12 differentiated into osteoblasts by bone morphogenic protein 2. Furthermore, in the organ-cultured tooth germs, the liver-type TNSALP mRNA was expressed according to differentiation of tooth germs. Conclusion: These results suggest that the liver-type TNSALP mRNA is induced according to differentiation of bone and tooth
Solution Properties of Gellan Gum: Change in Chain Stiffness between Single- and Double-Stranded Chains
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Simple Colorimetric Inhibition Assay of Heme Crystallization for High-Throughput Screening of Antimalarial Compounds
No full textCurrent assays for screening new antimalarials need initiators of β-hematin formation that require laborious preparation, special devices, and substrates. In this study, based on reduction of heme absorption in β-hematin formation, we developed a simple colorimetric assay using Tween 20 as an initiator and a microplate reader for high-throughput screening of inhibitors of β-hematin formation
