Substantiation of conditions for biomodification of the muscle tissue of sea cucumber in preparation of mayonnaise sauces

Conditions for biomodification of the muscle tissue of sea cucumber are substantiated to reduce the portion of unprocessed raw material in the final product - sauce. For this purpose, enzymatic hydrolysis of the tissue with proteases Protamex and CelloLux is applied before the main processing. Effect of these enzymes on structural and mechanical properties of the final product is defined, as well as the effect of stabilizers. Rational concentrations of enzymes are determined for the final products with certain viscosity

Antiradical effect of low-molecular peptides in extracts and hydrolyzates from tissues of water organisms

Molecular-weight composition of peptides in extracts and hydrolyzates from soft tissues of the clams Corbicula japonica and Mercenaria mercenaria and liver of chum salmon is investigated. Antiradical activity is defined for low-molecular peptides, as well as for high-molecular proteins and free amino acids in the extracts. The maximum activity is detected for the extracts of salmon liver; all protein fractions in the water extract of the liver have antiradical activity. Low-molecular peptides and free amino acids have antiradical activity in all samples. The peptides with molecular weight 4.3 kDa have the highest activity in the water extract from C. japonica (43 units) and the peptides with molecular weight 4.7 kDa - in the water extract from M. mercenaria (5.6 units). After hydrolysis, the portion of low-molecular peptides increases for C. japonica , M. mercenaria , and salmon liver in 22.1, 14.5, and 11.1 %, respectively. Hence antiradical activity for hydrolyzates from C. japonica and M. mercen aria is in 1.9 times higher and for hydrolyzates from salmon liver - in 1.3 times higher than for water extracts from their tissues. Only the peptides with molecular weight 2.8-4.7 kDa have antiradical activity in the hydrolyzates; its value is 1-24 activity units for M. mercenaria ; 13-76 units for C. japonica and about 40 units for the chum salmon liver. Correlation is found between the content of peptides with molecular weight 3-4 kDa in hydrolyzates and their antiradical activity

Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module

To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena sp. PCC 7120 (termed AnaCTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoid-mediated homodimerization, yielding violet–purple protein samples. We characterize the spectroscopic properties of the obtained pigment–protein complexes and the thermodynamics of liposome–protein carotenoid transfer and demonstrate the delivery of carotenoid echinenone from AnaCTDH into liposomes with an efficiency of up to 70 ± 3%. Most importantly, we show efficient carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species (ROS). Incubation of neuroblastoma cell line Tet21N in the presence of 1 μM AnaCTDH binding echinenone decreased antimycin A ROS production by 25% (p < 0.05). The described carotenoprotein may be considered as part of modular systems for the targeted antioxidant delivery.BMBF, 01DJ15007, Carotenoidbindende photoschaltbare Proteine: Lichtinduzierte Dynamik und Anwendungen in modernen mikroskopischen Verfahre

Отходы переработки дальневосточных голотурий как сырье для получения биологически активных добавок к пище

Экспериментально установлены условия ферментативного гидролиза отходов, полученных при разделке промысловых голотурий, добываемых в морских акваториях российского Дальнего Востока. Показано, что гидролизаты содержат высокое количество тритерпеновых гликозидов, а также отличаются разнообразным составом макро- и микроэлементов, что позволяет позиционировать их как источник полезных минералов. Содержание селена настолько высоко, что 2-5 г лиофилизированного гидролизата фактически соответствуют рекомендованной суточной дозе этого элемента. Гидролизаты обладают высокой антиоксидантной активностью. Их органическая составляющая более чем на половину представлена биологически активными пептидами с молекулярной массой 2,5-5,0 кДа. Полученные результаты свидетельствуют о перспективности использования отходов переработки голотурий для получения биологически активных добавок широкого спектра действия