5 research outputs found
Substantiation of conditions for biomodification of the muscle tissue of sea cucumber in preparation of mayonnaise sauces
Conditions for biomodification of the muscle tissue of sea cucumber are substantiated to reduce the portion of unprocessed raw material in the final product - sauce. For this purpose, enzymatic hydrolysis of the tissue with proteases Protamex and CelloLux is applied before the main processing. Effect of these enzymes on structural and mechanical properties of the final product is defined, as well as the effect of stabilizers. Rational concentrations of enzymes are determined for the final products with certain viscosity
Antiradical effect of low-molecular peptides in extracts and hydrolyzates from tissues of water organisms
Molecular-weight composition of peptides in extracts and hydrolyzates from soft tissues of the clams Corbicula japonica and Mercenaria mercenaria and liver of chum salmon is investigated. Antiradical activity is defined for low-molecular peptides, as well as for high-molecular proteins and free amino acids in the extracts. The maximum activity is detected for the extracts of salmon liver; all protein fractions in the water extract of the liver have antiradical activity. Low-molecular peptides and free amino acids have antiradical activity in all samples. The peptides with molecular weight 4.3 kDa have the highest activity in the water extract from C. japonica (43 units) and the peptides with molecular weight 4.7 kDa - in the water extract from M. mercenaria (5.6 units). After hydrolysis, the portion of low-molecular peptides increases for C. japonica , M. mercenaria , and salmon liver in 22.1, 14.5, and 11.1 %, respectively. Hence antiradical activity for hydrolyzates from C. japonica and M. mercen aria is in 1.9 times higher and for hydrolyzates from salmon liver - in 1.3 times higher than for water extracts from their tissues. Only the peptides with molecular weight 2.8-4.7 kDa have antiradical activity in the hydrolyzates; its value is 1-24 activity units for M. mercenaria ; 13-76 units for C. japonica and about 40 units for the chum salmon liver. Correlation is found between the content of peptides with molecular weight 3-4 kDa in hydrolyzates and their antiradical activity
Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module
To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena sp. PCC 7120 (termed AnaCTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoid-mediated homodimerization, yielding violetβpurple protein samples. We characterize the spectroscopic properties of the obtained pigmentβprotein complexes and the thermodynamics of liposomeβprotein carotenoid transfer and demonstrate the delivery of carotenoid echinenone from AnaCTDH into liposomes with an efficiency of up to 70 Β± 3%. Most importantly, we show efficient carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species (ROS). Incubation of neuroblastoma cell line Tet21N in the presence of 1 ΞΌM AnaCTDH binding echinenone decreased antimycin A ROS production by 25% (p < 0.05). The described carotenoprotein may be considered as part of modular systems for the targeted antioxidant delivery.BMBF, 01DJ15007, Carotenoidbindende photoschaltbare Proteine: Lichtinduzierte Dynamik und Anwendungen in modernen mikroskopischen Verfahre
ΠΡΡ ΠΎΠ΄Ρ ΠΏΠ΅ΡΠ΅ΡΠ°Π±ΠΎΡΠΊΠΈ Π΄Π°Π»ΡΠ½Π΅Π²ΠΎΡΡΠΎΡΠ½ΡΡ Π³ΠΎΠ»ΠΎΡΡΡΠΈΠΉ ΠΊΠ°ΠΊ ΡΡΡΡΠ΅ Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ Π΄ΠΎΠ±Π°Π²ΠΎΠΊ ΠΊ ΠΏΠΈΡΠ΅
ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Ρ ΡΡΠ»ΠΎΠ²ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π° ΠΎΡΡ
ΠΎΠ΄ΠΎΠ², ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΏΡΠΈ ΡΠ°Π·Π΄Π΅Π»ΠΊΠ΅ ΠΏΡΠΎΠΌΡΡΠ»ΠΎΠ²ΡΡ
Π³ΠΎΠ»ΠΎΡΡΡΠΈΠΉ, Π΄ΠΎΠ±ΡΠ²Π°Π΅ΠΌΡΡ
Π² ΠΌΠΎΡΡΠΊΠΈΡ
Π°ΠΊΠ²Π°ΡΠΎΡΠΈΡΡ
ΡΠΎΡΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΠΠ°Π»ΡΠ½Π΅Π³ΠΎ ΠΠΎΡΡΠΎΠΊΠ°. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π°ΡΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ Π²ΡΡΠΎΠΊΠΎΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΡΡΠΈΡΠ΅ΡΠΏΠ΅Π½ΠΎΠ²ΡΡ
Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ΄ΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΠΎΡΠ»ΠΈΡΠ°ΡΡΡΡ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·Π½ΡΠΌ ΡΠΎΡΡΠ°Π²ΠΎΠΌ ΠΌΠ°ΠΊΡΠΎ- ΠΈ ΠΌΠΈΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ², ΡΡΠΎ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°ΡΡ ΠΈΡ
ΠΊΠ°ΠΊ ΠΈΡΡΠΎΡΠ½ΠΈΠΊ ΠΏΠΎΠ»Π΅Π·Π½ΡΡ
ΠΌΠΈΠ½Π΅ΡΠ°Π»ΠΎΠ². Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΠ΅Π»Π΅Π½Π° Π½Π°ΡΡΠΎΠ»ΡΠΊΠΎ Π²ΡΡΠΎΠΊΠΎ, ΡΡΠΎ 2-5 Π³ Π»ΠΈΠΎΡΠΈΠ»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π°ΡΠ° ΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡ ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄ΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΡΡΠΎΡΠ½ΠΎΠΉ Π΄ΠΎΠ·Π΅ ΡΡΠΎΠ³ΠΎ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠ°. ΠΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π°ΡΡ ΠΎΠ±Π»Π°Π΄Π°ΡΡ Π²ΡΡΠΎΠΊΠΎΠΉ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ. ΠΡ
ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠΎΡΡΠ°Π²Π»ΡΡΡΠ°Ρ Π±ΠΎΠ»Π΅Π΅ ΡΠ΅ΠΌ Π½Π° ΠΏΠΎΠ»ΠΎΠ²ΠΈΠ½Ρ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π° Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΠΏΠ΅ΠΏΡΠΈΠ΄Π°ΠΌΠΈ Ρ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠΉ ΠΌΠ°ΡΡΠΎΠΉ 2,5-5,0 ΠΊΠΠ°. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΎΡΡ
ΠΎΠ΄ΠΎΠ² ΠΏΠ΅ΡΠ΅ΡΠ°Π±ΠΎΡΠΊΠΈ Π³ΠΎΠ»ΠΎΡΡΡΠΈΠΉ Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ
Π΄ΠΎΠ±Π°Π²ΠΎΠΊ ΡΠΈΡΠΎΠΊΠΎΠ³ΠΎ ΡΠΏΠ΅ΠΊΡΡΠ° Π΄Π΅ΠΉΡΡΠ²ΠΈΡ