1 research outputs found
Unraveling Tamarindus indica Pulp-Derived Green Magnesium Oxide Nanoparticles for Cardioprotective Potential against Doxorubicin-Induced Cardiomyopathy: A Comprehensive Biochemical and Gene Expression Study
The present work investigates a sustainable approach
to synthesize
magnesium oxide nanoparticles (MgO NPs) using an aqueous pulp extract
derived from Tamarindus indica. The
effective synthesis of MgO NPs was verified by characterizing methods
such as UV–vis spectroscopy, X-ray diffraction (XRD), Fourier
transform infrared (FT-IR) spectroscopy, and scanning electron microscopy–energy-dispersive
X-ray spectroscopy (SEM-EDX). These nanoparticles possess small crystallite
sizes, distinctive surface shapes, specific elemental compositions,
and stabilizing and encapsulating constituents. Furthermore, total
phenolic content (TPC) and total flavonoid content (TFC) tests revealed
the existence of phytochemical components in MgO NPs. Significantly,
these MgO NPs demonstrated exceptional antioxidant capabilities, as
evidenced by their strong performance in antioxidant assays such as
2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic
acid) (ABTS), nitric oxide (NO) scavenging, and iron chelation tests.
They also exhibited a notable ability to inhibit red blood cell (RBC)
hemolysis and lipid peroxidation. In toxicity assessments using Baby
Hamster Kidney fibroblasts (BHK-21) and Vero cell lines, the MgO NPs
displayed a safe profile. Additionally, in vivo studies
on Doxorubicin (DOX)-induced cardiotoxicity revealed the cardioprotective
properties of these NPs, accompanied by a detailed understanding of
the underlying mechanisms. Pretreatment with MgO NPs effectively countered
DOX-induced alterations in cardiac biomarkers, lipid profiles, cardiac
enzymes, and lipid peroxidation. Furthermore, they modulated apoptosis-related
markers (caspase-3 and p53), upregulated antiapoptotic (Bcl-2), and
antioxidant (SOD) markers, suggesting their potential therapeutic
value in addressing DOX-induced cardiomyopathy. In conclusion, this
study underscores the promising cardioprotective, hypolipidemic, antioxidant,
and antiapoptotic qualities of MgO NPs derived from tamarind pulp,
offering valuable insights into their therapeutic applications and
underlying biological mechanisms