Leptin supplementation in embryo culture medium increases in vivo implantation rates in mice

Leptin is a hormone-like protein consisting of 167 amino acids. The aim of this study is to compare the effects of different leptin concentrations on in vitro and in vivo embryo development rates. In vitro development rates were investigated by embryo culture studies, and in vivo implantation rates and the quality of embryos were assessed by embryo transfers to the recipient mice. The results showed that addition of leptin into the embryo culture medium at 10 and 100 ng/mL doses supported the in vitro development of mouse embryo. Moreover, leptin increased the total cell number of blastocyst, particularly the trophectoderm cells. In vivo assessment showed a significant increase in the proportion of the embryos implanted in 10 and 100 ng/mL groups, compared to the control group. In conclusion, leptin supplement in embryo culture medium increases implantation rates in mice

Comparison of the development of mouse embryos manipulated with different biopsy techniques

Preimplantation genetic diagnosis is the detection of inherited diseases and the sex of embryos before implantation in the practice of human medicine as well as in veterinary medicine. The introduction of experimental animal embryo biopsy techniques has been a milestone in the developmental process of preimplantation genetic diagnosis techniques. The aim of the present study was to evaluate in vivo and in vitro development of embryos after biopsy in an experimental mouse model and to perform comparisons across different biopsy techniques (blastomere biopsy and trophectoderm biopsy). At the end of the study, no significant difference was observed between the blastomere biopsy group and the control group in terms of in vitro development, embryo quality, and fetal development, whereas embryo quality and in vivo development were negatively affected in the trophectoderm biopsy group (P < 0.05)

Comparison of the development of mouse embryos manipulatedwith different biopsy techniques

Preimplantation genetic diagnosis is the detection of inherited diseases and the sex of embryos before implantation in the practice of human medicine as well as in veterinary medicine. The introduction of experimental animal embryo biopsy techniques has been a milestone in the developmental process of preimplantation genetic diagnosis techniques. The aim of the present study was to evaluate in vivo and in vitro development of embryos after biopsy in an experimental mouse model and to perform comparisons across different biopsy techniques (blastomere biopsy and trophectoderm biopsy). At the end of the study, no significant difference was observed between the blastomere biopsy group and the control group in terms of in vitro development, embryo quality, and fetal development, whereas embryo quality and in vivo development were negatively affected in the trophectoderm biopsy group (P < 0.05)

The Effect of Solid Surface Vitrification (SSV) Versus Classic Vitrification Technique on Survive Rate of in vitro Produced Bovine Blastocysts

The aim of this study was to compare Solid Surface Vitrification (SSV) technique and classic vitrification technique in in vitro produced 8 days old bovine blastocysts. Cryopreservation of mammalian embryo has great importance for genetic resources conservation, embryo transfer, veterinary and clinical reproductive biotechnology and animal assisted reproductive technologies. Immature oocytes were maturated then fertilized with frozen-thawed bull semen and cultured until blastocyst stage in commercial sequential culture medium for 8 days. Blastocysts were vitrified in two different groups as SSV and classic vitrification and non-vitrified blastocysts were used as control group. After vitrification, vitrified blastocysts were warmed and cultured for 1 day. For this aim, blastocyst viability rate and median cell number were investigated. The blastocyst viability rate that vitrified by classic vitrification (34.8%) were found to be lower than those vitrified by SSV (82.6%1 and control group blastocysts (100%). However, median cell numbers of vitrified-warmed blastocysts were found higher in SSV (124) than classic vitrification (104). Median cell number of control group was detected as 213. As a result blastocyst viability rate and median cell number in SSV group was higher than classic vitrification group, there was a significant difference between SSV and classic vitrification group (p<0.05)

Optimal Method of Mouse Blastomere Biopsy: In vitro Developmental Potential of the Biopsied Embryo to Blastocyst Stage after Aspirated Eight-Cell Mouse Embryos

The researchers investigated the effect of blastocyst development and quality of blastomere aspiration techniques on eight-cell mouse embryos. The results clearly indicate that the in vitro development of biopsied mouse embryos depended on the suitable aspiration method. This method related to preimplantation genetic diagnosis for the genesis of animal models for animal disorders from embryos. In this study, female CB6 F1 (Balb/cXC57b1/j) hybrid mice were superovulated with hormones and superovulated females were sacrified approximately 68 h after hCG administration. About eight-cell embryos were recovered from oviducts of sacrified mouse in M2 medium. Before biopsy, all embryos were incubated to decrease cell to cell contacts to microdrops of Ca2+/Mg2+ free QAM HTF (3 mg mL(-1) BSA Fraction V) with HEPES for 90 min at 37 degrees C. A single blastomere of eight-cell embryos were aspirated by the aspiration pippetes under inverted microscope (4X). After biyopsy, embryos were cultured in SAGE medium supplemented with 5% CO2, 5% O-2 and 90% air at 37 degrees C for up to expanded blastocyst stage. After culture, 267 biopsied embryos out of 234 (88.32 +/- 7.5%) expanded blastocysts developed from the biopsy group and total cell number mean 67.8 +/- 17.2% were recorded in the experiment group and 126 non-biopsied embryos out of 118 (94.4 +/- 7.78%) expanded blastocysts developed from control embryos and total cell number mean 70 +/- 15.4% was recorded in the control group. In the present study, there was no difference in blastocysts developmental rates at post biopsy group and control group (p = 0.05). In conclusion, the biopsy the method described here is an optimal method of blastomere aspiration on in vivo mouse embryos