YOUNG ADOLESCENT EFL LEARNERS’ PERSPECTIVES ON CRITICAL THINKING SKILLS

Critical Thinking Skills (CTs) are among the 21st century learning skills, and schools are expected to equip the students with these skills.  Turkey has been restructuring the educational system in order to improve the quality of education which enables students to acquire such learning skills as critical and creative thinking, problem solving, and collaboration. The present study based on the perspectives of Young Adolescent EFF learners presents findings on the students’ awareness of CTs, and whether or not they apply them to a given task, and if there is any conflict between knowledge and application of CTs. The findings showed that the students, despite their quiet well awareness, they did not effectively apply CTs. The problems they encountered were assumed to be resulted from lacking in metacognitive knowledge

Osteoarthritis: Omentin

Objective: Knee osteoarthritis (KOA) is defined as a chronic degenerative joint disease. Obesity is a significant risk factor for KOA. Omentin is an adipose tissue-induced adipokine. The aim of the present study was to investigate the correlation between obesity and serum omentin levels in patients with KOA.Methods: This study included 60 patients with KOA, 34 obese individuals (O-KOA) and 26 nonobese individuals (NO-KOA) and 40 controls, 17 obese individuals (NOC) and 23 nonobese individuals (NOC) matched in terms of age, sex, and body mass index (BMI) who were recruited from the same polyclinic. Blood samples and knee radiographs were obtained from all the subjects, and clinical features, BMI, and laboratory parameters were recorded. The Kellgren-Lawrence (KL) grade and Western Ontario and McMaster Universities Osteoarthritis (WOMAC) index were used to classify the radiographic and clinical findings, respectively. Serum omentin levels were determined using an ELISA.Results: Serum omentin levels in patients were significantly lower than those in the controls (p < 0.05). When the BMI values and KL scores were considered, serum omentin levels significantly decreased in severe O-KOA versus in mild-to-moderate O-KOA. There was no statistically significant decrease in severe NO-KOA vercus mild-to-moderate NO-KOA. There was a significant negative correlation between the serum omentin level and BMI and WOMAC index. All findings were supported by a receiver operating characteristic curve analysis.Conclusion: Serum omentin levels were inversely related to obesity and the severity of KOA. The data indicate that omentin may be a new biomarker of KOA to our knowledge and may aid the diagnosis of early-stage O-KOA, if our findings are supported by further studies involving much more samples.C1 [Gundogdu, Gulsah] Pamukkale Univ, Fac Med, Dept Physiol, TR-20070 Denizli, Turkey.[Gundogdu, Koksal] Denizli State Hosp, Dept Orthoped & Traumatol, Denizli, Turkey.[Miloglu, Fatma Demirkaya] Ataturk Univ, Fac Pharm, Dept Analyt Chem, Erzurum, Turkey.[Tasci, Seymanur Yilmaz] Ataturk Univ, Fac Med, Dept Physiol, Erzurum, Turkey

Colorectal Adenocarcinoma Cells

Objective: Isoorientin (ISO) is a flavonoid compound extracted from plant species. The goal of this study was to determine the potential antiproliferative effects of ISO in HT-29 human colorectal adenocarcinoma cell line in vitro, specifically on cell viability, apoptosis, and cell cycle pathways.Materials and Methods: The cytotoxic effect of ISO isolated from E. spectabilis was measured using 2,3-bis(2methoxy-4-nitro-5-sulfophenyl)-2-H-tetrazolium-5-carboxanilide (XTT) assay in HT-29 cell lines. Total RNA was isolated using Tri-Reagent protocol. The effects of ISO on apoptosis-related gene were detected using real-time polymerase chain reaction (RT-PCR). The findings were analyzed using "Delta-Delta CT" Delta Delta CT method and evaluated using a computer program. Volcano plot analysis was used for comparing groups and the data obtained were statistically analyzed using Student t test.Results: According to XTT result analysis, the 50% inhibitory concentration (IC50) value of ISO was 125 mu M at the 48th h in HT-29 cells. The RT-PCR analysis in HT-29 cells showed that Cyclin D1 (CCND1), Cyclin-dependent kinase 6 (CDK6), BAX, BCL-2, Checkpoint kinase 1-2 (CHEK1, CHEK2) and Excision repair cross-complementing 1 (ERCC1) expressions were reduced in ISO-treated cells compared with those in the control group of cells. P53, P21, Caspase-3 (CASP-3), Caspase-8 (CASP-8), and Caspase-9 (CASP-9) gene expressions were increased Ataxia Telengiectasia and Rad-3 related (ATR) was activated in the ISO-treated group of cells compared with those in the control group of cells (p< 0.05).Conclusion: ISO affected the proliferation of colorectal cancer (CRC) cells via cell cycle pathways. It also altered apoptosis gene expression. These results demonstrated that ISO can be a therapeutic agent for CRC treatment; however, more studies are needed to investigate its mechanism of actions