ERGs of wild type (WT) and Pika-/- mice after injection of PBS or APB/PDA.

<p>(A) Photopic ERGs series recorded at each minute for 9 minutes of light adaptation. The ERGs were elicited by a stimulus intensity of 1.0 log cd-s/m² after injection of PBS or APB/PDA. The ERGs recorded from WT are shown on the left and those of Pika-/- mice are shown on the right. (B) Time-response curves of the mean amplitudes of the a- and b-waves of 11 WT mice and 11 Pika-/- mice after intravitreal injection of PBS. The curve for the WT mice is shown on the upper left and that for Pika-/- mice is shown on lower left respectively. Time-response curves of the mean amplitudes of a-wave of 11 WT mice and 11 Pika-/- mice after intravitreous injection of APB + PDA are shown in the upper right. Photopic photoreceptor potentials after the injection of APB+PDA during light adaptation were not significantly different between WT and PIKA-/- mice (P >0.05: repeated measures ANOVA). ● = ERGs of WT mice and ○ = ERGs of Pika-/- mice. The means ± SDs are plotted.</p

Effect of different stimulus intensities on the amplitudes of the a- and b-waves of the scotopic (A) and photopic (B) ERGs of wild type (WT) and Pika-/- mice.

<p>(A) Scotopic flash ERGs elicited by 11 different stimulus intensities (from -5.0 to 1.0 log cd-s/m²), and photopic flash ERGS elicited by 9 different stimulus intensities (from -1.4 to 1.0 log cd-s/m²) recorded in a representative WT mouse and a representative Pika-/- mouse. (B) The intensity–response curves of the mean scotopic and photopic b-wave amplitudes of five WT mice and five Pika-/- mice are shown in the upper left and lower left panels respectively. The amplitudes of the scotopic b-wave of WT mice were not significantly different from that of Pika-/- mice (P >0.05: repeated measures ANOVA). However, the amplitudes of the photopic b-wave of Wt mice were significantly different from Pika-/- mice (P <0.05: repeated measures ANOVA). In addition, the intensity–response curves of the mean scotopic and photopic b-wave implicit times are shown in the upper right and lower right panel respectively. The implicit times of the scotopic and photopic b-waves of WT mice were significantly different from Pika-/- mice (P <0.05: repeated measures ANOVA).The means ± SDs are plotted. ● = WT mice and ○ = Pika-/- mice.</p

Extracted post-receptoral components of WT and Pika-/- mice.

<p>(A) Waves were obtained by subtracting the averaged ERGs of the 11 eyes injected with APB + PDA from those of 11 eyes injected with PBS. The subtracted waveforms at each minute for 9 minutes of light adaptation of WT mice and Pika-/- mice, which indicated post-receptoral component, are shown. (B) Amplitudes of maximum positive wave of the subtracted waveforms are plotted during the course of light adaptation of WT mice and Pika-/- mice.</p

ERGs of wild type (WT) and Pika-/- mice during the light adaptation.

<p>(A) Photopic ERGs recorded at each minute for 9 minutes of light adaption (40 cd/m²) from a representative WT mouse and a Pika-/- mouse. ERGs were elicited by two different stimulus intensities, 0.4 and 1.0 log cd-s/m². ERGs recorded at each minute for 9 minutes of light adaptation (black trace) are superimposed on the ERG elicited at the start of light adaptation (0 min). (B) Time-response curves of the mean a- and b-wave amplitudes of 5 WT mice and 5 Pika-/- mice are shown in the right and left panel respectively. The amplitudes of the photopic a- and b-waves during light-adaptation of WT mice were significantly different from that of Pika-/- mice (P <0.05: repeated measures ANOVA). ● = ERGs of WT mice recorded with 1.0 log cd-s/m². ○ = ERGs of Pika-/- mice recorded with 1.0 log cd-s/m². ▲ = ERGs of WT mice recorded with 0.4 log cd-s/m². △ = ERGs of Pika-/- mice recorded with 0.4 log cd-s/m². The means ± SDs are plotted.</p

ERGs of WT and Pika-/- mice after injection of PBS or bicuculline.

<p>(A) Photopic ERGs series recorded at each minute for 9 minutes of light adaptation. ERGs elicited by stimulus intensity of 1.0 log cd-s/m² after injection of PBS or bicuculline. The ERGs recorded from WT are shown on the left and those of Pika-/- mice are shown on the right. (B) Time-response curves of the mean amplitudes of the a- and b-waves of 8 WT mice and 6 Pika-/- mice after intravitreal injection of PBS or bicuculline are shown in the right and left panel respectively. In Pika-/- mice, the amplitudes of the photopic b-wave were significant different during light adaptation during light adaptation between PBS and bicuculline injection groups (P <0.05: repeated measures ANOVA). ● = ERGs of WT mice injected with PBS. ○ = ERGs of Pika-/- mice injected with PBS. ■ = ERGs of WT mice injected with bicuculline. □ = ERGs of Pika-/- mice injected with bicuculline. The means ± SDs are plotted.</p