Исследование сравнительной фармакокинетики и биоэквивалентности препаратов Кардиоприл таблетки, 20 мг (АО «Химфарм», Республика Казахстан) и Моноприл® таблетки, 20 мг («Бристол-Майерс Сквибб С.Р.Л.», Италия)

In a single-dose, two-treatment, two-period, two-sequence crossover study with a 1-week washout period was carry out the bioequivalence study of two tablet coated formulation of fosinopril that given to 18 volunteers in equal doses (20 mg). Drug blood plasma concentrations were determined by validated LC-MS method for 48 hours. There were calculated the followed parameters: AUC 0-t ,Cmax, tmax , Cmax /AUC. 90% confidence interval for log-transformed AUC 0-tvalues was 0,9393 - 1,1473 and one for log-transformed Cmax was 0,8861 - 1,066, respectively. It was made the conclusion about bioequivalence of compared fosinopril formulations.В рамках перекрёстного, однократного, открытого, рандомизированного исследования с однонедельным периодом отмывки, с двумя последовательностями была изучена биоэквивалентность двух таблетированных форм фозиноприла на 18 добровольцах (дозировка 20 мг). Образцы плазмы крови анализировали валидированным методом ВЭЖХ-МС/МС в течение 48 часов. Для анализируемых препаратов рассчитаны следующие фармакокинетические параметры: AUC0-t, Cmax, tmax, Cmax/AUC. 90% доверительный интервал для логарифмически преобразованных значений AUC0-t составил 0,9393 - 1,1473 и для Cmax - 0,8861 - 1,066. По результатам исследования был сделан вывод о биоэквивалентности сравниваемых препаратов фозиноприла

Исследования биоэквивалентности Нипезама ретард таблетки, 400 мг (АО «Химфарм», Республика Казахстан) и Финлепсина® 400 ретард таблетки, 400 мг («Плива Краков, Фармацевтическая компания С.А.», Польша)

In a single-dose, two-treatment, two-period, two-sequence crossover study with a 1-month washout period was carry out the bioequivalence study of two tablet coated formulation of carbamazepine that given to 18 volunteers in equal doses (400 mg). Drug blood plasma concentrations were determined by validated LC-UV method for 120 hours. There were calculated the followed parameters: AUC0-t, Cmax , Tmax, Cmax /AUC. 90% confidence interval for log-transformed AUC. values was 0,93693 - 1,10204 and one for log-transformed Cmax was 0,91045 - 1,12287, respectively. It was made the conclusion about bioequivalence of compared carbamazepine formulations.В рамках перекрёстного, однократного, открытого, рандомизированного исследования с одномесячным периодом отмывки, с двумя последовательностями была изучена биоэквивалентность двух таблетированных форм карбамазепина на 18 добровольцах (дозировка 400 мг). Образцы плазмы крови анализировали валидированным методом ВЭЖХ-УФ в течение 120 часов. Для анализируемых препаратов рассчитаны следующие фармакокинетические параметры: AUC0-t, Cmax, Tmax, Cmax/AUC. 90% доверительный интервал для логарифмически преобразованных значений AUC0-t составил 0,93693 - 1,10204 и для Cmax - 0,91045 - 1,12287. По результатам исследования был сделан вывод о биоэквивалентности сравниваемых препаратов карбамазепина

Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding.

New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectrometry and an investigation of the hybridization properties of the decamers. We show that the modified γ-S-carboxyethyl-T10 PNA forms a stable triplex with polyadenine DNA

A clinical case of SYNGAP1, с2214_2217delTGAG de novo gene mutations in a girl with epilepsy, mental retardation, autism, and movement disorders

The practical introduction of the latest genetic techniques could provide the basis for comorbidity of genetic epilepsies and behavioral disorders with cognitive impairments. This article describes a case of SNGAP1 mutation in an 8-year-old female patient having symptomatic epilepsy with epileptic eyelid myoclonia and atypical absences, atypical autism with mental retardation. A detailed clinical discussion deals with neurological and mental states, logopedic characteristics, and psychological examination findings, as well as video-EEG monitoring data

Chromatographic profiles of the PNA monomers (enantiomers).

<p>(A) Mixture of <b>4a</b> and <b>4b</b> (<i>S</i>- and <i>R</i>- enantiomers of the γ-monomer); (B) <i>S</i>-entantiomer of the γ-monomer, <b>4a</b> (Conditions: Diasphere column 110-Chirasel-E-PA, 7 μm; elution system: MeOH/CH₃COOH = 96/4 (v/v); flow rate: 1 mL/min; UV-detection at 254 nm; temperature: 20°C); (C) Mixture of <b>5a</b> and <b>5b</b> (<i>R</i>- and <i>S</i>-enantiomers of the α-monomer); (D) <i>S</i>-entantiomer of the α-monomer, <b>5a</b> (Conditions: Diasphere column 110-Chirasel-E-PA, 7 μm; elution system: MeOH/CH₃COOH/TEA = 100/0.1/0.1 (v/v/v); flow rate: 0.5 mL/min; UV-detection at 254 nm; temperature: 20°C); R = CH₂CH₂COOBn.</p

J-Plots and CD spectra of γ-<i>ce</i>-H-(T₁₀)-Gly-NH₂ (2)/dA₁₀.

<p>(A) Absorbance of the γ-<i>ce</i>-H-(T₁₀)-Gly-OH (<b>2</b>)/dA₁₀ mixtures with various PNA/DNA ratios at 260 nm; (B) Absorbance of the mixtures at 275 nm; (C) Molar ellipticity at 263 nm. Conditions: 10 mM Na₂HPO₄ (pH 7.4), 140 mM KCl, 5 mM MgCl₂. The summed PNA and DNA concentration was 7 μM at each ratio; (D) CD spectra of the triplex. The molar ellipticity is given per 1 nucleotide in a strand. Conditions: 10 mM Na₂HPO₄ (pH 5 or 7.4), 140 mM KCl, 5 mM MgCl₂. The triplex concentration was 1.8 μM.</p

Chromatographic profiles of the PNA monomer derivatives (diastereomers).

<p>(A) Mixture of diastereomers <b>7a</b> and <b>7b</b>; (B) Diastereomer <b>7a</b> (Conditions: Luna column, 5 μm, CN 100A, elution system: heptane/isopropanol (88/12), flow rate: 1 mL/min); (C) Mixture of diastereomers <b>8a</b> and <b>8b</b>; (D) Diastereomer <b>8a</b> (Conditions: Separon SGX RP-S C18 column, 5 μm, 4x150 mm, elution system: acetonitrile/water (1/1), flow rate: 1 mL/min); R = -CH₂CH₂COOBn.</p

UV-melting and CD spectra of PNA/DNA and DNA/DNA complexes.

<p>(A) Thermal difference spectra of the complexes; (B) Thermal denaturation profiles of the complexes; (C) CD spectra of the single-stranded oligonucleotides and PNAs. The molar ellipticity was calculated per 1 nucleotide. (D) CD spectra of the complexes. Conditions: 10 mM Na₂HPO₄ (pH 7.4), 140 mM KCl, 5 mM MgCl₂. The concentration of each PNA or oligonucleotide was 2.5 μM. The CD spectra were measured at 15°C.</p

Schematic representation of PNA and DNA fragments.

<p>(A) DNA fragment; (B) <i>aeg</i>-, γ- and α-<i>ce</i>-PNAs; (C) Starting thymine monomers <b>4</b> and <b>5</b> and their conformers, which coexist in solution due to the reduced rotation along the N^<b>tert</b>-C(O)-bond.</p

Age, growth and preliminary estimates of maturity of bigeye tuna, Thunnus obesus, in the Australian region

Biological parameters such as age, growth and age (or size) at maturity are vital for accurate stock assessments and management plans to ensure that fisheries develop sustainably. Despite this, very few validated age studies have been conducted for large tropical pelagic species within the Australian region. Age and growth parameters were estimated for bigeye tuna, Thunnus obesus (Lowe, 1839), sampled from longline fisheries in the Australian region using validated techniques based on counts of annual increments. Poor increment clarity reduced the number of otoliths included in the final analysis to only 50% of the 3200 selected for reading (39–178-cm fork length). Microincrement analysis confirmed the position of the first two annual increments in these otoliths.A maximum age of 16 years was obtained, but over 80% of fish in the Australian catch were <5 years old. Growth is most rapid in the first few years of life and asymptotic length is reached at about age 9 to 10 years. The von Bertalanffy growth parameters were estimated at L∞ =169.09, k=0.238, and to=−1.706 for the south-west Pacific Ocean and L∞ =178.41, k=0.176, and to=−2.500 for the eastern Indian Ocean. These parameters were significantly different, suggesting that there is little mixing between populations in the Pacific and Indian Oceans. Length at 50% maturity for females sampled in northern Queensland was estimated to be 102.4-cm fork length