Antimalarial dihydrochalcone isolated from Artocarpus sericicarpus Jarret leaves and in silico investigation against falcipain-2 protein

Aims: To investigate the potential antimalarial plant belonging to the Artocarpus genus, Artocarpus sericicarpus, and isolate the antimalarial active compound from leaves extract. Methods: The isolation of the antimalarial compound was based on bioassay-guided isolation. The compound isolation was conducted by chromatography and identified using spectroscopic techniques, including 1H, 13C, and 2D NMR. The compound was tested for its antimalarial activity against Plasmodium falciparum and evaluated for cytotoxicity using several cell lines, including Huh7, HepG2, BHK-21, and Vero cells. In silico investigation of the compound against falcipain-2 protein was conducted as well. Results: Fractionation and purification of dichloromethane leaves extract led to the isolation of a dihydrochalcone compound identified as 1-(2,4-dihydroxyphenyl)-3-[4-hydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one. The dihydrochalcone compound exhibited antimalarial activity with an IC50 value of 34.80 µM. Cytotoxicity test revealed CC50 values of the compound were more than 20 µg/mL, and the selectivity indexes (SI) were 4.50, 5.52, 3.02, and 6.68 on Huh7, HepG2, BHK-21, and Vero cells, respectively. The CC50 and SI indicated the nontoxic criteria of the compound. In silico investigation showed that the compound could bind to the falcipain-2 active sites. Conclusions: The dihydrochalcone compound identified as 1-(2,4-dihydroxyphenyl)-3-[4-hydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one was isolated from Artocarpus sericicarpus leaves extract showed antimalarial activity and the ability to bind to the falcipain-2 active sites on in silico investigation

Pengembangan Media Pembelajaran Buku Saku Berbasis Mind Mapping Tematik Tema 5 Subtema 1 di Kelas V SD Negeri 106810 Sampali T.A 2022/2023

Penelitian ini bertujuan untuk mengetahui kevalidan, kepraktisan, dan ke keefektifan media pembelajaran Buku Saku Berbasis Mind Mapping pada pembelajaran Tema 5 subtema 1 komponen Ekosistem Kelas V SD Negeri 106810 Sampali T.A 2022/2023. Penelitian ini menggunakan model pengembangan ADDIE. Teknik pengumpulan data dalam penelitian ini menggunakan observasi, wawancara, angket dan tes. Kefektifan produk di ukur dari hasil belajar siswa melaui intstrumen pre test dan post test. Hasil kevalidan oleh validator ahli materi sebesar 90% termasuk kategori “sangat layak” dan ahli media sebesar 86% masuk dalam kategori sangat layak. Penilaian oleh pendidik memberikan 92% masuk kategori “sangat layak”. Hasil belajar siswa Pretest dan Post test menggunakan Media Buku Saku Berbasis Mind Mapping adanya peningkatan dari 52,64 menjadi 89,28. Hasil uji N-Gain score 0,88 dengan persentasi 88,29% dengan kriteria “sangat efektif”. Dapat disimpulkan bahwa Media Buku Saku Berbasis Mind Mapping layak digunakan menjadi media pembelajaran tematik dan dapat meningkatkan hasil belajar siswa di kelas V SD Negeri 106810 Sampali