The maternal leucocytes in thrombophilia and hypothyroidism and their influence on fetal cells

© 2020, University of Kragujevac, Faculty of Science. All rights reserved. The literature data show that thrombophilia and maternal dysfunction of thyroid gland during pregnancy are associated with an increased risk of miscarriage, placental abruption, hy-pertensive disorders and fetal growth retardation. It was shown that thyroid hormones and hypercoagulable states influence onto a leucocyte activity. The aim of this study has been to investigate maternal leucocytes changes and their correlation with frequency of fetal cells micronuclei in pregnant women with thrombophilia and hypothyroidism. The samples of blood and amniotic fluid were collected from healthy pregnant women and pregnant women with inherited thrombophilia and hypothy-roidism (16-18 weeks of gestation). Hematological characteristics were determined by using standard hematological methods. The frequency of micronuclei was determined in fetal cells after amniocentesis by using standard cytogenetic methods. The results of this study showed significant higher levels of β-hCG, number of monocytes and eosinophils in blood of pregnant women with thrombophilia. A large number of eosinophils was documented in blood of pregnant women with hypothyroidism. Increased percentage distribution of eosinophils and basophils is shown in both investigated groups of pregnant women. The increased fetal cells micronuclei frequency and their correlation with percentage distribution of eosinophils and basophils were indicated in pregnant women with hypothyroidism. The obtained results suggest that an increased percentage of eosino-phils and basophils in pregnant women with hypothyroidism contribute to a formation of micronuclei in fetal cells

Colletotrichum Linicola - Osetljivost različitih genotipova lucerke

Southern anthracnose or crown rot, caused by Colletotrichum linicola, is a disease that has been detected on alfalfa in Serbia. During longer period, especially in summer and autumn, alfalfa plants with anthracnose symptoms were studied in the field. Stem infection resulted in wilting and death of the upper portion of the steam, giving rise to the characteristic “shepherd`s crook” symptom.To examine the level of sensitivity of different alfalfa genotypes in experimental conditions, one isolate: Coll-44 (C. linicola originating from Serbia). Ten commercial alfalfa genotypes of different origin (K-1, K-28, Zajecarska 83, Osjecka 12, NS Slavija, Banja Luka, Affinity 401 + Z, Florida 77, Vernal S and Perry) were examined in this study. Infected plants showed typical symptoms of anthracnose. Necrotic lesions appeared on the plant stems and gentle bending of the top of the upper third of stems occurred on tested plants. Necrotic lesions further spread onto the whole plant and in some plants led to withering. Assessment of damage by pathogen inoculation was based on the scale of 0-5. Investigated genotypes showed contrasting resistances to C. linicola isolates.Anthraknoza ili trulež krune lucerke, izazvana gljivoma it roda Colletotrichum, je bolest koja nanosi značajne štete na lucerki u Srbiji. U toku višegodišnjeg perioda, u toku leta i rane jeseni, sakupljane su biljke lucerke sa simptomima antraknoze. Stabljične infekcije izazivaju sušenje i povijanje gornjeg dela biljke, što dovodi do karakterističnog simptoma tzv. "pastirska kuka". Ispitivanje stepena osetljivosti različitih genotipova lucerke u eksperimentalnim uslovima obavljeno je sa jednim izolatom Coll-44 (C. linicola poreklom iz Srbije). Deset komercijalnih genotipova lucerke različitog geografskog porekla (K-1, K-28, Zaječarska 83, Osječka 12, NS Slavija, Banja Luka, Affinity 401 + Z, Florida 77, Vernal S i Pery) ispitivano je u ovom radu. Inokulisane biljake lucerke pokazale su tipične simptome antraknoze. Nekrotične lezije pojavile su sa na stabljikama zaraženih biljaka i došlo je do povijanja u gornjoj trećini stabala. Micelija parazita prorasta niz stablo i kada se stabljike osuše, nastupa antraknoza krune i korena. Osetljivost različitih genotipova lucerke prema ispitivanim izolatima C.linicola određena je prema skali od 0-5. Ispitivani genotipovi lucerke su ispoljili različitu osetljivost prema testiranom izolatu vrste C. linicola

Prinos suve mase lucerke u zavisnosti od sorte, faze razvića i otkosa

The main objective of this study was to assess dry matter productivity of different alfalfa cultivars depending on stage of maturity and cut, as well as the optimal time of harvest to achieve the maximum yield. Dry matter yield of alfalfa and red clover depended on all three factors and their interactions. The local variety of alfalfa, K 28, was more productive (from 6,8 to 2,3 t ha-1 from first to fourth cut, and from 3,4 to 5,5 t ha-1 from first to third stage of development) compared to US variety, G+13R+CZ (from 5,9 to 2,2 t ha-1 from first to fourth cut, and from 2,7 to 5,2 t ha-1 from first to third stage of development). The maximum yield of K 28 variety could be achieved by cutting alfalfa five times a year.Cilj ovih istraživanja je bio da se utvrdi produktivnost različitih sorti lucerke u zavisnosti od faze razvića i otkosa, kao i optimalno vreme košenja radi postizanja maksimalnog prinosa. Prinos suve materije lucerke zavisio je od sva tri ispitivana faktora i njihovih interakcija. Domaća sorta lucerke je produktivnija (6,8 do 2,3 t ha-1 od prvog do četvrtog otkosa, odnosno od 3,4 do 5,5 t ha-1 od prve do treće faze razvića) u odnosu na američku (5,9 do 2,2 t ha-1 od prvog do četvrtog otkosa, odnosno od 2,7 do 5,2 t ha-1 od prve do treće faze razvića). Maksimalan prinos sorte K-28 mogao bi se postići kosidbom pet puta godišnje

Floristički sastav i prinos biomase travnjaka Danthonietum calycinae pod uticajem đubrenja i kalcizacije

The aim of this study was evaluated change of botanical composition and dry matter yield influenced by different fertilizers (control, P60K60, N.60P60K60) and lime (without lime and 1000 kg ha-1 lime) of Danthonietum calycinae grassland type, in central part of Serbia. Nitrogen fertilisation treatments led to an increase of grass species, but also decreased plants from other families. Tretmant P60K60 with lime stimulated an increase of legumes in biomass in first and second year of investigation. Aplication of nitrogen increase forage yield in both years of investigation. Effect of P60K60 tretmans and lime on increasing dry matter yield was observed in second year.Cilj rada je bio ispitivanje uticaja đubrenja i kalcizacije na floristički sastav i prinos biljne zajednice Danthonietum calycinae, u centralnom delu Srbije. Ispitivani su tretmani đubrenja (kontrola, P60K60 i N60P60K60) i kalcifikacije (bez kreča i 1000 kg ha-1). Na tretmanina na kojima je unešen mineralni azot došlo je do porasta udela trava, a pada udela biljaka ostalih familija. Najpovoljniji uslovi za razvoj leguminoza bili su na tretmanima PK sa krečom, u obe godine istraživanja. Povoljan uticaj mineralnog azota uočen je već u prvoj godini, dok je unošenje fosfornih i kalijumovih đubriva dalo efekta na prinos tek u drugoj godini istraživanja