ヒト細胞増殖に対する外部磁場の影響

金沢大学薬学部近年, 人体が外部磁場にさらされる機会が急増し, 人体に対する磁場の影響が問題となってきている. そこでその基礎研究として,ヒト細胞増殖と光化学反応に対する外部磁場効果を検討した.1.HeLa S3細胞の塩殖に対する磁場効果ヒトがん細胞の増殖に対する磁場効果を検討した. 永久磁石の組み合わせにより定常磁場0.2Tと交流磁場(0.1T, 30Hz)を発生した. ヒトがん細胞HeLa S3を37°Cの培養室内で約1週間増殖し, その間の細胞数を計数したが, 定常磁場・交流磁場いずれも有意な差異は認められなかった. 次にX線照射との協同作用について検討した. 定常磁場下づX線照射後磁場中で細胞増殖を行った. コロニー形成・増殖曲線には有意な効果はみられなかったが, セルサイクルをしらべたところX線を照射した系ではセルサイクルに異常が認められた. その原因について今後更に検討の予定である.2.光化学反応に対する磁場効果SDSミセル水溶液中のキノキザリン, キサントン, クロモン等の光誘起水素引抜き反応に対する磁場効果をレーザー〓光法等により研究した. いずれの場合も反応中間作の寿命, 収量が磁場により増大, 反応中間作のラジカル対の三重項-一重項項間交差が磁場により仰制されることが明らかとなった.研究課題/領域番号:62602516, 研究期間(年度):1987出典：研究課題「ヒト細胞増殖に対する外部磁場の影響」課題番号62602516（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-62602516/）を加工して作

カルボニル類の分子内水素引抜き反応の磁場効果

金沢大学薬学部研究課題/領域番号60571015, 研究期間(年度):1985出典：研究課題「カルボニル類の分子内水素引抜き反応の磁場効果」課題番号60571015（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-60571015/）を加工して作

有機光化学反応の磁場効果

金沢大学薬学部光化学反応に対する磁場効果の研究は新しいタイプの反応の制御法として, また反応機構の新しい解析法として注目を集めている. そこで光電子移動反応を中心に, その外部磁場効果を磁場変調けい光法, 〓〓秒時間分解けい光法・レーザー閃光法等により研究し, 磁場効果のメカニズムを詳細に研究した.1.分子内光電子移動反応の磁場効果フェナントレン(Ph)とジメチルアニリン(DMA)をメチレン鎖末端にもつモデル化合物(Ph-n-DMA, n=3〜10)について検討した. Phの励〓により, 分子内エキサイプレックスけい光が観測される. このけい光に対する磁場効果とその溶媒効果・メチレン鎖長効果を調べた. その結果:溶媒の誘電率が8程度で光電子移動によるラジカルイオン対が生成し, そのラジカルイオン対の一重項一三重項項問交差が磁場により抑制されることが分った. またラジカルイオン対の一重項と三重項状態はメチレン鎖10以上で起こることが示された.2.分子間光電子移動反応の磁場効果PhとDMAの分子間エキサイプレックスけい光に対する磁場効果を変調磁場けい光法により研究した. 新しく開発したこの方法は1%以下の極めて小さな磁場効果の検出に有用なことが分った. 分子間エキサイプレックスけい光の磁場効果は, DMAの〓度により変化するが, そのメカニズムについて解明した.3.ポリマー内光電子移動反応の磁場効果PhとDMAを側鎖にもつポリマーのエキサイプレックスけい光に対する磁場効果を研究し, 長距離の光電子移動により一部エキサイプレックスが生成することが示された.研究課題/領域番号:62540322, 研究期間(年度):1987出典：研究課題「有機光化学反応の磁場効果」課題番号62540322（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-62540322/）を加工して作

Crystal morphology change by magnetic susceptibility force

We found a change in morphology when lysozyme crystals were grown in a magnetic field. The phenomenon was caused by the magnetic force derived from the magnetic susceptibility gradient. We propose that this force should be called the “magnetic susceptibility force"

光化学反応に及ぼす磁場効果の研究

金沢大学薬学部研究課題/領域番号:X00210----274141, 研究期間(年度):1977出典：「光化学反応に及ぼす磁場効果の研究」研究成果報告書　課題番号X00210----274141（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-X00210----274141/）を加工して作

Effect of olmesartan on the levels of circulating endothelial progenitor cell after drug-eluting stent implantation in patients receiving statin therapy

AbstractBackgroundThe endothelial progenitor cell (EPC) plays an important role in repairing vascular injury. Statins and angiotensin II receptor blockers increase the level of circulating EPCs. However, it is unknown whether the angiotensin II receptor blocker olmesartan synergistically acts with statins to increase the levels of circulating EPCs. Moreover, the association between the levels of circulating EPCs and endothelial dysfunction after implantation of drug-eluting stents (DESs) has not been evaluated.MethodsNine patients with stable coronary artery disease underwent percutaneous coronary intervention (PCI) and received DES implantation. All patients received olmesartan in addition to statin therapy after PCI. The dose of olmesartan was based on the physician's discretion as per the patients’ blood pressure. The levels of circulating EPCs were analyzed at baseline, post-PCI, and 1, 2, 3, and 8 months after PCI. Coronary angiography and the acetylcholine provocation test were performed on all patients at 8 months.ResultsAlthough the angiotensin II level significantly changed, the levels of circulating EPCs did not change during 8 months of olmesartan treatment (3.1±0.6cells/ml, 2.5±0.8cells/ml, 2.0±0.6cells/ml, 2.9±0.9cells/ml, 3.0±0.4cells/ml, 3.4±0.8cells/ml, p=0.64). The patients were subsequently divided into two groups based on whether the level of circulating EPCs was less or greater than 4cells/ml at 8 months. There were no significant differences in the mean vessel diameter of each segment (proximal, proximal edge, distal edge, and distal) after the acetylcholine provocation test between the two groups.ConclusionsLow-to-moderate doses of olmesartan might not increase the level of circulating EPCs in patients receiving statin therapy. There might be no association between the levels of circulating EPCs and the degree of coronary vasospasm in the acetylcholine provocation test 8 months after DES implantation

Effect of horizontal strong static magnetic field on swimming behaviour of Paramecium caudatum

Effect of horizontal strong static magnetic field on swimming behavior of Paramecium caudatum was studied by using a superconducting magnet. Around a center of a round vessel, random swimming at 0 T and aligned swimming parallel to the magnetic field (MF) of 8 T were observed. Near a wall of the vessel, however, swimming round and round along the wall at 0 T and aligned swimming of turning at right angles upon collision with the wall, which was remarkable around 1~4 T, were detected. It was experimentally revealed that the former MF-induced parallel swimming at the vessel center was caused physicochemically by the parallel magnetic orientation of the cell itself. From magnetic field dependence of the extent of the orientation, the magnetic susceptibility anisotropy (χ‖-χ⊥) was first obtained to be 3.4×10-23 emu cell-1 at 298 K for Paramecium caudatum. The orientation of the cell was considered to result from the magnetic orientation of the cell membrane. On the other hand, although mechanisms of the latter swimming near the vessel wall regardless of the absence and presence of the magnetic field are unclear at present, these experimental results indicate that whether the cell exists near the wall alters magnetic field effect on the swimming in the horizontal magnetic field

Effects of high magnetic field on Euglena gracilis

The effects of high magnetic field on Euglena gracilis Z were examined. When a horizontal magnetic field gradient (ca. 400 T2m-1) was applied, living E. gracilis moved to a higher field (positive magnetotaxis), whereas dead one gathered in a lower field. Magnetotaxis was not observed in a uniform magnetic field of 8 T. E. gracilis was oriented almost perpendicularly to the magnetic field regardless of life and death. Magnetotaxis of E. gracilis would be explained by taking into account inhomogeneous magnetic forces on and magnetic orientation of E. gracilis

From chemical gardens to chemobrionics

Chemical gardens are perhaps the best example in chemistry of a self-organizing nonequilibrium process that creates complex structures. Many different chemical systems and materials can form these self-assembling structures, which span at least 8 orders of magnitude in size, from nanometers to meters. Key to this marvel is the self-propagation under fluid advection of reaction zones forming semipermeable precipitation membranes that maintain steep concentration gradients, with osmosis and buoyancy as the driving forces for fluid flow. Chemical gardens have been studied from the alchemists onward, but now in the 21st century we are beginning to understand how they can lead us to a new domain of self-organized structures of semipermeable membranes and amorphous as well as polycrystalline solids produced at the interface of chemistry, fluid dynamics, and materials science. We propose to call this emerging field chemobrionics