7 research outputs found
Synthetic DNA-based Swimmers Driven by Enzyme Catalysis
Here, we report DNA-based synthetic nanostructures decorated
with
enzymes (hereafter referred to as DNAâenzyme swimmers) that
self-propel by converting the enzymatic substrate to the product in
solution. The DNAâenzyme swimmers are obtained from tubular
DNA structures that self-assemble spontaneously by the hybridization
of DNA tiles. We functionalize these DNA structures with two different
enzymes, urease and catalase, and show that they exhibit concentration-dependent
movement and enhanced diffusion upon addition of the enzymatic substrate
(i.e., urea and H2O2). To demonstrate the programmability
of such DNA-based swimmers, we also engineer DNA strands that displace
the enzyme from the DNA scaffold, thus acting as molecular âbrakesâ
on the DNA swimmers. These results serve as a first proof of principle
for the development of synthetic DNA-based enzyme-powered swimmers
that can self-propel in fluids
Synthetic DNA-based Swimmers Driven by Enzyme Catalysis
Here, we report DNA-based synthetic nanostructures decorated
with
enzymes (hereafter referred to as DNAâenzyme swimmers) that
self-propel by converting the enzymatic substrate to the product in
solution. The DNAâenzyme swimmers are obtained from tubular
DNA structures that self-assemble spontaneously by the hybridization
of DNA tiles. We functionalize these DNA structures with two different
enzymes, urease and catalase, and show that they exhibit concentration-dependent
movement and enhanced diffusion upon addition of the enzymatic substrate
(i.e., urea and H2O2). To demonstrate the programmability
of such DNA-based swimmers, we also engineer DNA strands that displace
the enzyme from the DNA scaffold, thus acting as molecular âbrakesâ
on the DNA swimmers. These results serve as a first proof of principle
for the development of synthetic DNA-based enzyme-powered swimmers
that can self-propel in fluids
Synthetic DNA-based Swimmers Driven by Enzyme Catalysis
Here, we report DNA-based synthetic nanostructures decorated
with
enzymes (hereafter referred to as DNAâenzyme swimmers) that
self-propel by converting the enzymatic substrate to the product in
solution. The DNAâenzyme swimmers are obtained from tubular
DNA structures that self-assemble spontaneously by the hybridization
of DNA tiles. We functionalize these DNA structures with two different
enzymes, urease and catalase, and show that they exhibit concentration-dependent
movement and enhanced diffusion upon addition of the enzymatic substrate
(i.e., urea and H2O2). To demonstrate the programmability
of such DNA-based swimmers, we also engineer DNA strands that displace
the enzyme from the DNA scaffold, thus acting as molecular âbrakesâ
on the DNA swimmers. These results serve as a first proof of principle
for the development of synthetic DNA-based enzyme-powered swimmers
that can self-propel in fluids
Synthetic DNA-based Swimmers Driven by Enzyme Catalysis
Here, we report DNA-based synthetic nanostructures decorated
with
enzymes (hereafter referred to as DNAâenzyme swimmers) that
self-propel by converting the enzymatic substrate to the product in
solution. The DNAâenzyme swimmers are obtained from tubular
DNA structures that self-assemble spontaneously by the hybridization
of DNA tiles. We functionalize these DNA structures with two different
enzymes, urease and catalase, and show that they exhibit concentration-dependent
movement and enhanced diffusion upon addition of the enzymatic substrate
(i.e., urea and H2O2). To demonstrate the programmability
of such DNA-based swimmers, we also engineer DNA strands that displace
the enzyme from the DNA scaffold, thus acting as molecular âbrakesâ
on the DNA swimmers. These results serve as a first proof of principle
for the development of synthetic DNA-based enzyme-powered swimmers
that can self-propel in fluids
Synthetic DNA-based Swimmers Driven by Enzyme Catalysis
Here, we report DNA-based synthetic nanostructures decorated
with
enzymes (hereafter referred to as DNAâenzyme swimmers) that
self-propel by converting the enzymatic substrate to the product in
solution. The DNAâenzyme swimmers are obtained from tubular
DNA structures that self-assemble spontaneously by the hybridization
of DNA tiles. We functionalize these DNA structures with two different
enzymes, urease and catalase, and show that they exhibit concentration-dependent
movement and enhanced diffusion upon addition of the enzymatic substrate
(i.e., urea and H2O2). To demonstrate the programmability
of such DNA-based swimmers, we also engineer DNA strands that displace
the enzyme from the DNA scaffold, thus acting as molecular âbrakesâ
on the DNA swimmers. These results serve as a first proof of principle
for the development of synthetic DNA-based enzyme-powered swimmers
that can self-propel in fluids
Synthetic DNA-based Swimmers Driven by Enzyme Catalysis
Here, we report DNA-based synthetic nanostructures decorated
with
enzymes (hereafter referred to as DNAâenzyme swimmers) that
self-propel by converting the enzymatic substrate to the product in
solution. The DNAâenzyme swimmers are obtained from tubular
DNA structures that self-assemble spontaneously by the hybridization
of DNA tiles. We functionalize these DNA structures with two different
enzymes, urease and catalase, and show that they exhibit concentration-dependent
movement and enhanced diffusion upon addition of the enzymatic substrate
(i.e., urea and H2O2). To demonstrate the programmability
of such DNA-based swimmers, we also engineer DNA strands that displace
the enzyme from the DNA scaffold, thus acting as molecular âbrakesâ
on the DNA swimmers. These results serve as a first proof of principle
for the development of synthetic DNA-based enzyme-powered swimmers
that can self-propel in fluids
Biodegradable Grubbs-Loaded Artificial Organelles for Endosomal Ring-Closing Metathesis
The application of transition-metal catalysts in living
cells presents
a promising approach to facilitate reactions that otherwise would
not occur in nature. However, the usage of metal complexes is often
restricted by their limited biocompatibility, toxicity, and susceptibility
to inactivation and loss of activity by the cellâs defensive
mechanisms. This is especially relevant for ruthenium-mediated reactions,
such as ring-closing metathesis. In order to address these issues,
we have incorporated the second-generation HoveydaâGrubbs catalyst
(HGII) into polymeric vesicles (polymersomes), which were composed
of biodegradable poly(ethylene glycol)-b-poly(caprolactone-g-trimethylene carbonate) [PEG-b-P(CL-g-TMC)] block copolymers. The catalyst was either covalently
or non-covalently introduced into the polymersome membrane. These
polymersomes were able to act as artificial organelles that promote
endosomal ring-closing metathesis for the intracellular generation
of a fluorescent dye. This is the first example of the use of a polymersome-based
artificial organelle with an active ruthenium catalyst for carbonâcarbon
bond formation