47 research outputs found
Uticaj tumor supresorskih gena TP53 i PTEN na odgovor na razliÄite naÄine leÄenja raka dojke
Rak dojke (RD) je najÄeÅ”Äi tip maligniteta i vodeÄi uzrok smrti od raka kod žena Å”irom sveta. RD je izuzetno heterogena bolest i stoga su neophodni razliÄiti modaliteti leÄenja da bi se pokrile ove razlike. Cilj naÅ”eg istraživanja je bio da se ispita uticaj inaktivacije TP53 i PTEN tumor supresorskih gena (TSG) na odgovor RD na razliÄite modalitete leÄenja, kao i njihova moguÄa saradnja u tome, na postoperativnim uzorcima RD. Metode. Pacijentkinje su klasifikovane, na osnovu primenjene adjuvantne terapije, u Äetiri razliÄite grupe: one koje su primale samo hormonsku terapiju (HT), hormonsku terapiju u kombinaciji sa hemoterapijom (HT/CHT), hormonsku terapiju u kombinaciji sa hemoterapijom i bioloÅ”kom terapijom (HT/CHT/H) i druge sistemske terapije koje iskljuÄuju HT. Funkcionalna inaktivacija TP53 i PTEN TSG je prouÄavana analizom mutacionog statusa, gubitka heterozigotnosti (LOH) i metilacionog statusa. Rezultati. NaÅ”i rezultati su pokazali da je TP53 gen izmenjen kod 63 od 90 pacijenata (70%), dok je uÄestalost promena PTEN gena bila neÅ”to niža, 54 od 90 (60%). Simultana inaktivacija je detektovana u 43 testirana uzorka (48%) sa znaÄajnom povezanoÅ”Äu izmeÄu dva analizirana TSG-a. Dalje, pokazali smo da status TP53 ima znaÄajan uticaj na odgovor pacijenata na terapiju. Suprotno ovome, nismo pokazali znaÄajnu asocijaciju izmeÄu mutacionog statusa PTEN-a i razliÄitih modaliteta leÄenja. MeÄutim, utvrÄena je znaÄajna povezanost izmeÄu primenjenih terapija i simultanih inaktivacija ova dva TSG-a (p = 0,00001). ZakljuÄak. Pacijenti sa wtTP53 pokazuju znaÄajno bolji terapijski odgovor bez obzira na vrstu terapije u poreÄenju sa nosiocima mutiranog TP53 gena.Introduction. Breast cancer (BC) is the most frequent type of malignancy and the leading cause of cancer related death among women worldwide. BC is exceptionally heterogeneous disease and therefore distinct treatment modalities are necessary to address these differences. The aim of our study was to investigate the impact of TP53 and PTEN tumor suppressor genes (TSGs) inactivation on BC response to different treatment modalities and their possible cooperation, on post-operative BC samples. Methods. Patients were classified, based on applied adjuvant therapy, into four distinct groups: those that received hormonal therapy (HT) only, hormonal therapy combined with chemotherapy (HT/CHT), hormonal therapy combined with chemo and biological therapy (HT/CHT/H), and other systemic therapies that exclude HT. Functional inactivation of TP53 and PTEN TSGās were studied by mutation, loss of heterozygosity (LOH) and hypermethylation analysis. Results. Our results revealed that TP53 gene was altered in 63 out of 90 specimens (70%), while the frequency of PTEN alterations was slightly lower, 54 out of 90 (60%). Simultaneous inactivation was detected in 43 tested samples (48%) with significant association between two analyzed TSGs. Further, we found that TP53 status has significant influence on patientsā therapy response. Contrary to this, no significance was found between mutational status of PTEN and various treatment modalities. However, significant association was found between the type of applied therapy and simultaneous alterations of these two TSGs (p = 0.00001). Conclusion. Patients with wtTP53 show significantly better therapy response regardless of the type of therapy, compared to carriers of altered TPp53 gene
TP53 and c-myc Co-alterations: A hallmark of oral cancer progression
Background: Head and neck squamous cell carcinoma, including oral cancer, is the sixth most common cancer worldwide. Despite advances in surgery and treatment, the 5-year survival rate has not improved significantly. Therefore, reliable molecular markers for oral cancer progression are badly needed. Methods: We conducted a copy number analysis to estimate amplification status of c-myc, cycD1 and EGFR oncogenes, mutational PCR-SSCP analysis to determine activation of H-ras oncogene and inactivation of TP53 tumour suppressor gene and methylation specific PCR analysis to evaluate hypermethylation of p16 and MGMT genes. Results: c-myc oncogene was amplified in 56.7%, cycDI in 20% and EGFR in 16.7% of Oral Squamous Cell Carcinoma (OSCC) cases while H-ras was activated in 33.3% of samples. Amplification of c-myc was significantly associated with the tumour grade 2. Interestingly, EGFR and H-ras alterations were mutually exclusive. p16 and MGMT were inactivated by hypermethylation in 30% and 13.3% of cases. Co-alteration of cycDI and p16 were not observed in any of the analyzed samples. TP53 was inactivated in 56.7% of samples and was significantly associated with progression of OSCC, grade 2 and stage 2. Moreover, TP53 and c-myc oncogene were simultaneously altered in grade 2 OSCC. Conclusions: The most promising marker of OSCC progression remains the TP53 tumour suppressor, which is the most frequently mutated gene in oral cancers. Since there is synergism between TP53 and c-myc, it seems that co-alteration of these two genes could be also a good marker of OSCC progression from grade1 to grade 2 tumours.Uvod: Skvamocelularni karcinomi glave i vrata (HNSCC) ukljuÄujuÄi i skvamocelularni karcinom usne duplje (OSCC) ubrajaju se u Å”est najÄeÅ”Äih tipova humanih maligniteta. Uprkos znaÄajnim napredcima u hirurÅ”kom i terapijskom tretmanu, stopa petogodiÅ”njeg preživljavanja kod ovog tipa maligniteta nije znaÄajnije popravljena. Upravo zato, definisanje pouzdanih molekularnih markera progresije kod OSSC predstavlja apsolutni prioritetet. Metode: Amplifikacioni status c-myc, cycD1 i EGFR gena odreÄen je pomoÄu eseja za detekciju broja genskih kopija, aktivacija H-ras onkogena i inaktivacija TP53 tumor supresora odreÄena je PCR-SSCP mutacionom analizom, a hipermetilacija promotora p16 i MGMT gena je ispitana metil specifiÄnim PCR-om (MSP). Rezultati: Amplifikacija c-myc onkogena detektovana je kod 56,7%, cycD1 onkogena kod 20%, a EGFR onkogena kod 16,7% analiziranih oralnih skvamocelularnih carcinoma. Istovremeno, mutaciona aktivacija H-ras onkogena detektovana je kod 33,3% ispitanih uzoraka. Amplifikovani c-myc, statistiÄki znaÄajno korelira sa gradusom 2 OSCC. Posebno intrigantan je bio nalaz po kom se onkogene aktivacije u EGFR i H-ras genu meÄusobno iskljuÄuju. Hipermetilacija promotora p16 gena detektovana je kod 30%, a MGMT kod 13,3% analiziranih uzoraka. Ko-alteracije cycDI i p16 gena nisu zapažene ni u jednom od analiziranih uzoraka. Inaktivacija TP53 gena detektovana je kod 56,7% uzoraka i utvrÄeno je da statistiÄki znaÄajno korelira sa gradusom 2 i statusom 2 OSCC. Pored ovoga, utvrÄeno je da statistiÄki znaÄajan broj uzoraka gradusa 2, sa aktiviranim TP53 genom ima istovremeno aktiviran i c-myc onkogen. ZakljuÄak: TP53, najÄeÅ”Äe mutirani gen u oralnim karcinomima, ostaje za sada i najpouzdaniji marker progresije kod OSCC. Obzirom na detektovani sinergizam izmeÄu TP53 i c-myc gena, možemo reÄi da su istovremene promene u ova dva gena joÅ” pouzdaniji pokazatelj progresije OSSC iz gradusa 1 u gradus 2.Projekat ministarstva br. III 41031 i br. ON17304
Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer.
Breast cancer is the most commonly occurring malignancy and the leading cause of cancer-related death in women. Triple-negative breast cancer (TNBC) is the most aggressive subtype and is associated with high recurrence rates, high incidence of distant metastases, and poor overall survival. The aim of this study was to investigate the PI3K/PTEN/Akt/mTOR pathway as one of the most frequently deregulated pathways in cancer. We aimed to explore the impact of PI3K and mTOR oncogenes as well as the PTEN tumor suppressor on TNBC clinical behavior, prognosis, and multidrug resistance (MDR), using immunohistochemistry and copy number analysis by quantitative real-time PCR. Our results revealed that loss of PTEN and high expression of PI3K and mTOR proteins are associated with poor outcome of TNBC patients. PTEN deletions appeared as a major cause of reduced or absent PTEN expression in TNBC. Importantly, homozygous deletions of PTEN (and not hemizygous deletions) are a potential molecular marker of metastasis formation and good predictors of TNBC outcome. In conclusion, we believe that concurrent examination of PTEN/PI3K/mTOR protein expression may be more useful in predicting TNBC clinical course than the analysis of single protein expression. Specifically, our results showed that PTEN-reduced/PI3K-high/mTOR-high expression constitutes a 'high risk' profile of TNBC
MolekularnobioloŔke osobine oralnih skvamocelularnih karcinoma
Oral squamous cell carcinomas (OSCCs) are associated with poor prognosis, and despite advances in therapy approaches, no major improvement in survival has been achieved in the recent years. Efforts are now directed toward finding new biological markers that could predict tumor behavior more accurately. OSCCs, as the majority of malignant tumors, arise from progressive accumulation of genetic and epigenetic lesions, transforming normal cells into malignant. In this paper, an analysis of current studies directed to understanding the underlying mechanisms of OSCC pathogenesis was presented. The emphasis was put on mutational analysis of cancer genes, as well as on the role of viral infections and methylation processes in OSCC. Finally, an overview of studies that tried to determine the possibility for developing OSCC was given.Oralne skvamocelularne karcinome (OSCK) odlikuje uglavnom loÅ”a prognoza i, uprkos pomacima u terapijskim postupcima, poslednjih godina nije ostvaren napredak u preživljavanju osoba s ovim tumorom. Velike nade se polažu u molekularnu medicinu i pronalaženje novih bioloÅ”kih markera pomoÄu kojih bi preciznije nego Å”to to dopuÅ”taju kliniÄki i histopatoloÅ”ki parametri moglo da se predvidi ponaÅ”anje tumora. OSCK, kao i veÄina drugih malignih oboljenja, rezultat su postupne akumulacije raznovrsnih genetiÄkih i epigenetiÄkih promena u Äelijama, koje od normalnih postaju neoplastiÄne. U ovom radu dat je presek nekih od pravaca istraživanja na polju molekularne biologije oralnih karcinoma, s osvrtom na studije koje se bave ispitivanjem somatskih mutacija u kancerskim genima, uÄeÅ”Äem onkogenih virusa u patogenezi i znaÄaju procesa metilacije za OSCK. TakoÄe su pomenute studije posveÄene utvrÄivanju eventualnog postojanja predispozicije za razvoj OSCK
Moleculobiological characteristics of oral squamous cell carcinomas
Oral squamous cell carcinomas (OSCCs) are associated with poor prognosis, and despite advances in therapy approaches, no major improvement in survival has been achieved in the recent years. Efforts are now directed toward finding new biological markers that could predict tumor behavior more accurately. OSCCs, as the majority of malignant tumors, arise from progressive accumulation of genetic and epigenetic lesions, transforming normal cells into malignant. In this paper, an analysis of current studies directed to understanding the underlying mechanisms of OSCC pathogenesis was presented. The emphasis was put on mutational analysis of cancer genes, as well as on the role of viral infections and methylation processes in OSCC. Finally, an overview of studies that tried to determine the possibility for developing OSCC was given.Oralne skvamocelularne karcinome (OSCK) odlikuje uglavnom loÅ”a prognoza i, uprkos pomacima u terapijskim postupcima, poslednjih godina nije ostvaren napredak u preživljavanju osoba s ovim tumorom. Velike nade se polažu u molekularnu medicinu i pronalaženje novih bioloÅ”kih markera pomoÄu kojih bi preciznije nego Å”to to dopuÅ”taju kliniÄki i histopatoloÅ”ki parametri moglo da se predvidi ponaÅ”anje tumora. OSCK, kao i veÄina drugih malignih oboljenja, rezultat su postupne akumulacije raznovrsnih genetiÄkih i epigenetiÄkih promena u Äelijama, koje od normalnih postaju neoplastiÄne. U ovom radu dat je presek nekih od pravaca istraživanja na polju molekularne biologije oralnih karcinoma, s osvrtom na studije koje se bave ispitivanjem somatskih mutacija u kancerskim genima, uÄeÅ”Äem onkogenih virusa u patogenezi i znaÄaju procesa metilacije za OSCK. TakoÄe su pomenute studije posveÄene utvrÄivanju eventualnog postojanja predispozicije za razvoj OSCK.Projekat ministarstva br. 17507
Genomic instability as a prognostic marker in malignant brain cancer
Introduction: Glioblastoma and Astrocytoma are diff use malignant brain tumors and characterized as the most aggressive and invasive brain cancers. Glioblastoma IDH wild-type is a primary brain tumour that develops de novo, and Astrocytoma IDH mutant is a secondary tumour which arises by progression from lower tumour grades. They are characterized by poor survival, resistance to therapy and poor prognosis which develops as a consequence of genomic instability. Genomic instability also contributes to tumour heterogeneity and provides the genomic diversity necessary for selecĘ on. Materials and methods: 31 paĘ ents with Glioblastoma IDH wild-type and Astrocytoma IDH mutant, grade 3 and 4, were analysed for the presence of genomic instability using AP-PCR, DNA profi ling method. Comparing DNA profi les between tumour Ę ssue and normal Ę ssue (blood) of the same paĘ ent, we detected qualitaĘ ve and quanĘ taĘ ve changes. QualitaĘ ve changes are detected as the presence and absence of bands and are the manifestaĘ on of microsatellite instability (MIN). QuanĘ taĘ ve changes are the representaĘ on of chromosomal instability (CIN) and are detected as diff erences in the intensity of bands. Survival analyses were performed using Kaplan & Maier test for survival data in relaĘ on to diff erent histological tumour type and genomic instability. StaĘ sĘ cal diff erences were considered signifi cant for pā¤ 0,05. Results: PaĘ ents with Glioblastoma IDH wild-type have signifi cantly shorter survival compared to other histological types (p=0,025). For each histological type that we analysed and each type of instability MIN, CIN and total genomic instability, two groups of paĘ ents were made ā those with high and low instability. PaĘ ents with Glioblastoma IDH wild-type that have low total genomic instability have signifi cantly shorter survival (p=0,045) compared to other analysed types of brain cancer. PaĘ ents with Astrocytoma IDH mutant grade 4 who have high total genomic instability and high CIN have signifi cantly shorter survival (p=0,018, p=0,007 respecĘ ully). Conclusion: PaĘ ents with Glioblastoma IDH wild-type have shorter survival which makes this tumour the most aggressive and malignat of all analysed tumours. Our results show that low genomic instability in Glioblastoma IDH wild-type and high genomic instability lead by high CIN in Astrocytoma IDH mutant, gradus 4 contribute to shorter survival, which makes genomic instability a potential good prognostic markerThe first number of Oncology Insights includes Proceedings book of The Sixth Congress of the Serbian Association for Cancer Research with international participation (Oct 2-4, 2023, Belgrade
Moleculobiological characteristics of oral squamous cell carcinomas
Oral squamous cell carcinomas (OSCCs) are associated with poor prognosis, and despite advances in therapy approaches, no major improvement in survival has been achieved in the recent years. Efforts are now directed toward finding new biological markers that could predict tumor behavior more accurately. OSCCs, as the majority of malignant tumors, arise from progressive accumulation of genetic and epigenetic lesions, transforming normal cells into malignant. In this paper, an analysis of current studies directed to understanding the underlying mechanisms of OSCC pathogenesis was presented. The emphasis was put on mutational analysis of cancer genes, as well as on the role of viral infections and methylation processes in OSCC. Finally, an overview of studies that tried to determine the possibility for developing OSCC was given.Oralne skvamocelularne karcinome (OSCK) odlikuje uglavnom loÅ”a prognoza i, uprkos pomacima u terapijskim postupcima, poslednjih godina nije ostvaren napredak u preživljavanju osoba s ovim tumorom. Velike nade se polažu u molekularnu medicinu i pronalaženje novih bioloÅ”kih markera pomoÄu kojih bi preciznije nego Å”to to dopuÅ”taju kliniÄki i histopatoloÅ”ki parametri moglo da se predvidi ponaÅ”anje tumora. OSCK, kao i veÄina drugih malignih oboljenja, rezultat su postupne akumulacije raznovrsnih genetiÄkih i epigenetiÄkih promena u Äelijama, koje od normalnih postaju neoplastiÄne. U ovom radu dat je presek nekih od pravaca istraživanja na polju molekularne biologije oralnih karcinoma, s osvrtom na studije koje se bave ispitivanjem somatskih mutacija u kancerskim genima, uÄeÅ”Äem onkogenih virusa u patogenezi i znaÄaju procesa metilacije za OSCK. TakoÄe su pomenute studije posveÄene utvrÄivanju eventualnog postojanja predispozicije za razvoj OSCK.Projekat ministarstva br. 17507
TP53 and c-myc Co-alterations: A hallmark of oral cancer progression
Background: Head and neck squamous cell carcinoma, including oral cancer, is the sixth most common cancer worldwide. Despite advances in surgery and treatment, the 5-year survival rate has not improved significantly. Therefore, reliable molecular markers for oral cancer progression are badly needed. Methods: We conducted a copy number analysis to estimate amplification status of c-myc, cycD1 and EGFR oncogenes, mutational PCR-SSCP analysis to determine activation of H-ras oncogene and inactivation of TP53 tumour suppressor gene and methylation specific PCR analysis to evaluate hypermethylation of p16 and MGMT genes. Results: c-myc oncogene was amplified in 56.7%, cycDI in 20% and EGFR in 16.7% of Oral Squamous Cell Carcinoma (OSCC) cases while H-ras was activated in 33.3% of samples. Amplification of c-myc was significantly associated with the tumour grade 2. Interestingly, EGFR and H-ras alterations were mutually exclusive. p16 and MGMT were inactivated by hypermethylation in 30% and 13.3% of cases. Co-alteration of cycDI and p16 were not observed in any of the analyzed samples. TP53 was inactivated in 56.7% of samples and was significantly associated with progression of OSCC, grade 2 and stage 2. Moreover, TP53 and c-myc oncogene were simultaneously altered in grade 2 OSCC. Conclusions: The most promising marker of OSCC progression remains the TP53 tumour suppressor, which is the most frequently mutated gene in oral cancers. Since there is synergism between TP53 and c-myc, it seems that co-alteration of these two genes could be also a good marker of OSCC progression from grade1 to grade 2 tumours.Uvod: Skvamocelularni karcinomi glave i vrata (HNSCC) ukljuÄujuÄi i skvamocelularni karcinom usne duplje (OSCC) ubrajaju se u Å”est najÄeÅ”Äih tipova humanih maligniteta. Uprkos znaÄajnim napredcima u hirurÅ”kom i terapijskom tretmanu, stopa petogodiÅ”njeg preživljavanja kod ovog tipa maligniteta nije znaÄajnije popravljena. Upravo zato, definisanje pouzdanih molekularnih markera progresije kod OSSC predstavlja apsolutni prioritetet. Metode: Amplifikacioni status c-myc, cycD1 i EGFR gena odreÄen je pomoÄu eseja za detekciju broja genskih kopija, aktivacija H-ras onkogena i inaktivacija TP53 tumor supresora odreÄena je PCR-SSCP mutacionom analizom, a hipermetilacija promotora p16 i MGMT gena je ispitana metil specifiÄnim PCR-om (MSP). Rezultati: Amplifikacija c-myc onkogena detektovana je kod 56,7%, cycD1 onkogena kod 20%, a EGFR onkogena kod 16,7% analiziranih oralnih skvamocelularnih carcinoma. Istovremeno, mutaciona aktivacija H-ras onkogena detektovana je kod 33,3% ispitanih uzoraka. Amplifikovani c-myc, statistiÄki znaÄajno korelira sa gradusom 2 OSCC. Posebno intrigantan je bio nalaz po kom se onkogene aktivacije u EGFR i H-ras genu meÄusobno iskljuÄuju. Hipermetilacija promotora p16 gena detektovana je kod 30%, a MGMT kod 13,3% analiziranih uzoraka. Ko-alteracije cycDI i p16 gena nisu zapažene ni u jednom od analiziranih uzoraka. Inaktivacija TP53 gena detektovana je kod 56,7% uzoraka i utvrÄeno je da statistiÄki znaÄajno korelira sa gradusom 2 i statusom 2 OSCC. Pored ovoga, utvrÄeno je da statistiÄki znaÄajan broj uzoraka gradusa 2, sa aktiviranim TP53 genom ima istovremeno aktiviran i c-myc onkogen. ZakljuÄak: TP53, najÄeÅ”Äe mutirani gen u oralnim karcinomima, ostaje za sada i najpouzdaniji marker progresije kod OSCC. Obzirom na detektovani sinergizam izmeÄu TP53 i c-myc gena, možemo reÄi da su istovremene promene u ova dva gena joÅ” pouzdaniji pokazatelj progresije OSSC iz gradusa 1 u gradus 2.Projekat ministarstva br. III 41031 i br. ON17304
TP53 AND C-MYC CO-ALTERATIONS - A HALLMARK OF ORAL CANCER PROGRESSION
Background: Head and neck squamous cell carcinoma, including oral cancer, is the sixth most common cancer worldwide. Despite advances in surgery and treatment, the 5-year survival rate has not improved significantly. There fore, reliable molecular markers for oral cancer progression are badly needed. Methods: We conducted a copy number analysis to estimate amplification status of c-myc, cycD1 and EGFR oncogenes, mutational PCR-SSCP analysis to determine activation of H-ras oncogene and inactivation of TP53 tumour suppressor gene and methylation specific PCR analysis to evaluate hypermethylation of p16 and MGMT genes. Results: c-myc oncogene was amplified in 56.7%, cycD1 in 20% and EGFR in 16.7% of Oral Squamous Cell Carcinoma (OSCC) cases while H-ras was activated in 33.3% of samples. Amplification of c-myc was significantly associated with the tumour grade 2. Interestingly, EGFR and H-ras alterations were mutually exclusive. p16 and MGMT were inactivated by hypermethylation in 30% and 13.3% of cases. Co-alteration of cycD1 and p16 were not observed in any of the analyzed samples. TP53 was inactivated in 56.7% of samples and was significantly associated with progression of OSCC, grade 2 and stage 2. Moreover, TP53 and c-myc oncogene were simultaneously altered in grade 2 OSCC. Conclusions: The most promising marker of OSCC progression remains the TP53 tumour suppressor, which is the most frequently mutated gene in oral cancers. Since there is synergism between TP53 and c-myc, it seems that co-alteration of these two genes could be also a good marker of OSCC progression from grade1 to grade 2 tumours
Amplification of cycline D1, c-myc and EGFR oncogenes in tumour samples of breast cancer patients
Background: Breast cancer is the most common form of cancer in women. It arises from multiple genetic changes in oncogenes and tumor suppressor genes. Among so far studied oncogenes relatively few, including epdermal growth factor receptor (EGfR), cyclinD1 (CCNDÄ°)and c-myc, have been found to play an important role in progression of this type of human malignancy. The aim of this study was to examine the prognostic potential of CCND1, c-myc and EGFR amplification and their possible cooperation in breast carcinogenesis. Methods: Copy number analyses of CCND1 and c-myc genes were done by TaqMan based quantitative real time PCR. Amplification status of EGFR was determined by differential Pcr. Results: Amplification of CCND1, c-myc and EGFR oncogene has been found in 20.4%, 26.5% and 26.5% of breast cancer cases, respectively. Analysis showed that amplification of CCND1 oncogene was significantly associated with the stage II of disease while amplification of EGFR gene was significantly associated with overexpression of HER-2/neu. Tumour stage and expression of HER-2/neu appeared to be significant predictors of patient's outcome. Stage I patients lived significantly longer then stage III patients (p=0.04) while patients with HER-2/neu overexpression had worse prognoses and lived significantly shorter (p=0.001). Finally, survival of patients who underwent hormone therapy only was significantly longer (p=0.001) then survival of the rest of patients. Conclusions: Amplification of CCND1 or EGFR oncogene is associated with the progression of breast cancer and bad prognosis. No co-ordination in amplification of CCND1, c-myc and EGFR oncogenes were established in this cohort of breast cancer patients.Uvod: Kancer dojke je najÄeÅ”Äi tip maligniteta koji se javljaju kod žena. Tumori dojke nastaju kao rezultat akumulacije genetiÄkih promena kako u onkogenima tako i u tumor supresorskim genima. MeÄu mnogim onkogenima Äija je uloga u genezi tumora dojke ispitivana do danas, samo se neki smatraju znaÄajnim za razviÄe ovih karcinoma. U tu se grupu svakako ubrajaju receptor za epidermalni factor rasta (EGFR), c-myc i ciklinD1 (CCND1). Cilj rada je bio utvrditi prognostiÄki znaÄaj amplifikacije CCND1, c-myc i EGFR onkogena u razviÄu tumora dojke kao i eventualne meÄusobne koalteracije ovih gena. Metode: Amplifikacioni status CCND1 i c-myc gena odreÄen je kvantitativnim PCR-om u realnom vremenu, a amplifikacioni status EGFR onkogena je definisan diferencijalnim PCR-om. Rezultati: Amplifikacija CCND1 gena detektovana je kod 20.4%, a c-myc i EGFR onkogena kod 26.5% ispitanih uzoraka. Analize su pokazale da je amplifikacija CCND1 onkogena statistiÄki znaÄajno povezana sa stadijumom II tumora dojke kao i da amplifikacija EGFR-a znaÄajno korelira sa poveÄanom ekspresijom HER2/neu. Analize kliniÄkih i histopatoloÅ”kih parametara su jasno pokazale da stadijum tumora i nivo ekspresije HER2/neu gena predstavljaju znaÄajne pokazatelje daljeg toka bolesti, odnosno sudbine pacijenta. UtvrÄeno je da pacijentkinje sa tumorima dojke stadijuma I žive znaÄajno duže od onih sa tumorom stadijuma III (p= 0.04) kao i da pacijentkinje sa HER2/neu pozitivnim statusom imaju goru prognozu i žive znaÄajno krace (p=0.001). Na kraju, studija je pokazala da pacijentkinje podvrgnute samo hormonskoj terapiji imaju najbolju prognozu i žive znaÄajno duže od ostalih (p=0.001). ZakljuÄak: Amplifikacija CCND1 i EGFR onkogena je povezana sa loÅ”om prognozom i progresijom karcinoma dojke. U ispitivanom tumorskom uzorku nisu detektovane nikakve koalteracije CCND1, c-myc i EGFR onkogena.Projekat ministarstva br. III 41031 i br. ON17304