3 research outputs found
Evaluation of cell disruption for partial isolation of intracellular pyruvate decarboxylase enzyme by silver nanoparticles method
Candida tropicalis TISTR 5350 was used in the comparison of seven concentration levels of silver nanoparticles (0, 5, 10, 15, 20, 25, and 30 ÎŒg mlâ1) for cell disruption methods. The optimized cell disruption strategy was selected based on the optimal protein yield and biological activity. The maximum volumetric and specific pyruvate decarboxylase (PDC, EC 4.1.1.1) activities (0.53±0.05 U mlâ1 and 0.17±0.02 U mgâ1 protein, respectively) were observed at 15 ÎŒg mlâ1 silver nanoparticles. The silver nanoparticle concentration level of 15 ÎŒg mlâ1 was investigated further by comparing the reaction mixtures at different time intervals of 0, 1, 2, 3, 4, 5, and 6 min. The result showed that the highest specific PDC activity of 0.39±0.01 U mgâ1 protein was obtained from mixing for 3 min. This was not significantly different (Pâ€0.05) from other mixing time intervals