Neutrophil-to-lymphocyte ratio and incident end-stage renal disease in Chinese patients with chronic kidney disease: results from the Chinese Cohort Study of Chronic Kidney Disease (C-STRIDE)

Abstract Background Chronic kidney disease (CKD) leads to end-stage renal failure and cardiovascular events. An attribute to these progressions is abnormalities in inflammation, which can be evaluated using the neutrophil-to-lymphocyte ratio (NLR). We aimed to investigate the association of NLR with the progression of end stage of renal disease (ESRD), cardiovascular disease (CVD) and all-cause mortality in Chinese patients with stages 1–4 CKD. Methods Patients with stages 1–4 CKD (18–74 years of age) were recruited at 39 centers in 28 cities across 22 provinces in China since 2011. A total of 938 patients with complete NLR and other relevant clinical variables were included in the current analysis. Cox regression analysis was used to estimate the association between NLR and the outcomes including ESRD, CVD events or all-cause mortality. Results Baseline NLR was related to age, hypertension, serum triglycerides, total serum cholesterol, CVD history, urine albumin to creatinine ratio (ACR), chronic kidney disease-mineral and bone disorder (CKD-MBD), hyperlipidemia rate, diabetes, and estimated glomerular filtration rate (eGFR). The study duration was 4.55 years (IQR 3.52–5.28). Cox regression analysis revealed an association of NLR and the risk of ESRD only in patients with stage 4 CKD. We did not observe any significant associations between abnormal NLR and the risk of either CVD or all-cause mortality in CKD patients in general and CKD patients grouped according to the disease stages in particular. Conclusion Our results suggest that NLR is associated with the risk of ESRD in Chinese patients with stage 4 CKD. NLR can be used in risk assessment for ESRD among patients with advanced CKD; this application is appealing considering NLR being a routine test. Trial registration ClinicalTrials.gov Identifier NCT03041987. Registered January 1, 2012. (retrospectively registered) ( https://www.clinicaltrials.gov/ct2/show/NCT03041987?term=Chinese+Cohort+Study+of+Chronic+Kidney+Disease+%28C-STRIDE%29&rank=1 )https://deepblue.lib.umich.edu/bitstream/2027.42/148285/1/12967_2019_Article_1808.pd

Intrarenal Single-Cell Sequencing of Hepatitis B Virus Associated Membranous Nephropathy

To date, the pathogenesis of hepatitis B virus (HBV)-associated membranous nephropathy (MN) remains elusive. This study aimed to decipher the etiopathogenesis of HBV-associated MN by performing single-cell RNA sequencing (scRNA-seq) of kidney biopsy specimens from a patient with HBV-associated MN and two healthy individuals. We generated 4,114 intrarenal single-cell transcriptomes from the HBV-associated MN patient by scRNA-seq. Compared to healthy individuals, podocytes in the HBV-associated MN patient showed an increased expression of extracellular matrix formation-related genes, including HSPA5, CTGF, and EDIL3. Kidney endothelial cells (ECs) in the HBV-associated MN were enriched in inflammatory pathways, including NF-kappa B signaling, IL-17 signaling, TNF signaling and NOD-like receptor signaling. Gene ontology (GO) functional enrichment analysis and Gene Set Variation Analysis (GSVA) further revealed that differentially expressed genes (DEGs) of ECs from the HBV-associated MN patients were enriched in apoptotic signaling pathway, response to cytokine and leukocyte cell-cell adhesion. The up-regulated DEGs in glomerular ECs of HBV-associated MN patients were involved in biological processes such as viral gene expression, and protein targeting to endoplasmic reticulum. We further verified that the overexpressed genes in ECs from HBV-associated MN were mainly enriched in regulation of protein targeting to endoplasmic reticulum, exocytosis, viral gene expression, IL-6 and IL-1 secretion when compared with anti-phospholipase A2 receptor (PLA2R)-positive idiopathic membranous nephropathy (IMN). The receptor-ligand crosstalk analysis revealed potential interactions between endothelial cells and other cells in HBV-associated-MN. These results offer new insight into the pathogenesis of HBV-associated MN and may identify new therapeutic targets for HBV-associated MN

Analysis of shared ceRNA networks and related-hub genes in rats with primary and secondary photoreceptor degeneration

IntroductionPhotoreceptor degenerative diseases are characterized by the progressive death of photoreceptor cells, resulting in irreversible visual impairment. However, the role of competing endogenous RNA (ceRNA) in photoreceptor degeneration is unclear. We aimed to explore the shared ceRNA regulation network and potential molecular mechanisms between primary and secondary photoreceptor degenerations.MethodsWe established animal models for both types of photoreceptor degenerations and conducted retina RNA sequencing to identify shared differentially expressed long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs). Using ceRNA regulatory principles, we constructed a shared ceRNA network and performed function enrichment and protein–protein interaction (PPI) analyses to identify hub genes and key pathways. Immune cell infiltration and drug–gene interaction analyses were conducted, and hub gene expression was validated by quantitative real-time polymerase chain reaction (qRT-PCR).ResultsWe identified 37 shared differentially expressed lncRNAs, 34 miRNAs, and 247 mRNAs and constructed a ceRNA network consisting of 3 lncRNAs, 5 miRNAs, and 109 mRNAs. Furthermore, we examined 109 common differentially expressed genes (DEGs) through functional annotation, PPI analysis, and regulatory network analysis. We discovered that these diseases shared the complement and coagulation cascades pathway. Eight hub genes were identified and enriched in the immune system process. Immune infiltration analysis revealed increased T cells and decreased B cells in both photoreceptor degenerations. The expression of hub genes was closely associated with the quantities of immune cell types. Additionally, we identified 7 immune therapeutical drugs that target the hub genes.DiscussionOur findings provide new insights and directions for understanding the common mechanisms underlying the development of photoreceptor degeneration. The hub genes and related ceRNA networks we identified may offer new perspectives for elucidating the mechanisms and hold promise for the development of innovative treatment strategies

The Deviation Analysis and Application of Correlation Tracking Algorithm

Correlation tracking algorithm is the most widely used image tracking algorithm . Its main error sources include processing error and positioning error. In order to improve the accuracy of correlation tracking algorithm, a mathematical model of positioning error based on the theory of random walk and martingale is set up, and the average time of positioning error exceeds the range is presented. The image-interpolation method and surfaces-fitting method are put forward to suppress the positioning error and improve the accuracy. Simulation results show that: compared with the whole pixel tracking, quadratic interpolation tracking can double the accuracy, and quartic interpolation and surfaces-fitting can improve the tracking accuracy of about 4 times

The Deviation Analysis and Application of Correlation Tracking Algorithm

Correlation tracking algorithm is the most widely used image tracking algorithm . Its main error sources include processing error and positioning error. In order to improve the accuracy of correlation tracking algorithm, a mathematical model of positioning error based on the theory of random walk and martingale is set up, and the average time of positioning error exceeds the range is presented. The image-interpolation method and surfaces-fitting method are put forward to suppress the positioning error and improve the accuracy. Simulation results show that: compared with the whole pixel tracking, quadratic interpolation tracking can double the accuracy, and quartic interpolation and surfaces-fitting can improve the tracking accuracy of about 4 times

Asiaticoside ameliorates renal ischemia/reperfusion injury by promoting CD4+CD25+FOXP3+ treg cell differentiation

Ischemia/reperfusion injury (I/R) is the major cause of acute kidney injury, which becomes a global health problem. The effects of asiaticoside, as an anti-inflammatory drug, on renal ischemia-reperfusion injury have not been well defined.After the CD4+ cells were treated with asiaticoside, the CD4+CD25+FOXP3+ Treg cell differentiation was detected by flow cytometry. The viability and release of inflammatory factors of CD4+CD25+FOXP3+ Treg cell were detected by CCK-8 and ELISA. Renal I/R injury mice model was established, and the mice were pre-treated with asiaticoside or CD25 antibody or infused with Treg cells. The histological changes of renal tissue were evaluated by Hematoxylin-eosin, PAS, and Masson staining. The renal function markers were evaluated by colorimetry, the release of inflammatory factors was determined by ELISA. The Th17 and Treg cells in the blood and spleen were quantified by flow cytometry. The expressions of FOXP3 and RoR-γt in renal tissues were determined by western blotting.Asiaticoside promoted CD4+CD25+FOXP3+ Treg cell differentiation, increased the cell viability and down-regulated TNF-α, IL-1β, and IL-6, while up-regulated IL-10 of CD4+CD25+FOXP3+ Treg cells. Moreover, asiaticoside ameliorated the histological damage, decreased the Th17 cells and increased Treg cells, and down-regulated the TNF-α, IL-1β, IL-6, blood urea nitrogen, serum creatinine, and RoR-γt, while up-regulated IL-10 and FOXP3 of renal I/R injury mice. Effect of asiaticoside on renal I/R injury mice was reversed by CD25 antibody whose role was further reversed by Treg cell infusing.In conclusion, asiaticoside ameliorated renal I/R injury due to promoting CD4+CD25+FOXP3+ Treg cell differentiation

Influence of Feed Rate on the Performance of Hydrocyclone Flow Field

In order to clarify the influence of feed rate on a hydrocyclone flow field, numerical simulation was employed to model the influence of feed rate on the pressure field, velocity field, air column, turbulent kinetic energy, and split ratio. The results revealed that static pressure, tangential velocity, and radial velocity increased with an increase in the feed rate. When the feed rate at the inlet increases from 1 m/s to 5 m/s, the static pressure increases from 5.49 kPa to 182.78 kPa, tangential velocity increases from 1.97 m/s to 11.16 m/s, and radial velocity increases from 0.20 m/s to 1.16 m/s demonstrating that a high feed rate facilitated the strengthening separation of the flow field. Meanwhile, with the increase in the feed rate, the split ratio of the hydrocyclone decreased, indicating that the concentration effect of the hydrocyclone improved. Additionally, the formation time of the air column was reduced, and the flow field became more stable. Nevertheless, the axial velocity and the turbulent kinetic energy also increased with the increase in the feed rate, and the increase in the axial velocity reduced the residence time of the material in the hydrocyclone, which was not conducive to the improvement of separation accuracy. In addition, the increase in turbulent kinetic energy led to an increase in energy consumption, which was not conducive to the improvement of the comprehensive performance of the hydrocyclone. Therefore, choosing an appropriate feed rate is of great significance to the regulation of the flow field and the improvement of hydrocyclone separation performance

Influence of Feed Rate on the Performance of Hydrocyclone Flow Field

In order to clarify the influence of feed rate on a hydrocyclone flow field, numerical simulation was employed to model the influence of feed rate on the pressure field, velocity field, air column, turbulent kinetic energy, and split ratio. The results revealed that static pressure, tangential velocity, and radial velocity increased with an increase in the feed rate. When the feed rate at the inlet increases from 1 m/s to 5 m/s, the static pressure increases from 5.49 kPa to 182.78 kPa, tangential velocity increases from 1.97 m/s to 11.16 m/s, and radial velocity increases from 0.20 m/s to 1.16 m/s demonstrating that a high feed rate facilitated the strengthening separation of the flow field. Meanwhile, with the increase in the feed rate, the split ratio of the hydrocyclone decreased, indicating that the concentration effect of the hydrocyclone improved. Additionally, the formation time of the air column was reduced, and the flow field became more stable. Nevertheless, the axial velocity and the turbulent kinetic energy also increased with the increase in the feed rate, and the increase in the axial velocity reduced the residence time of the material in the hydrocyclone, which was not conducive to the improvement of separation accuracy. In addition, the increase in turbulent kinetic energy led to an increase in energy consumption, which was not conducive to the improvement of the comprehensive performance of the hydrocyclone. Therefore, choosing an appropriate feed rate is of great significance to the regulation of the flow field and the improvement of hydrocyclone separation performance

IL-26 promotes the proliferation and survival of human gastric cancer cells by regulating the balance of STAT1 and STAT3 activation.

Interleukin-26 (IL-26) is one of the cytokines secreted by Th17 cells whose role in human tumors remains unknown. Here, we investigated the expression and potential role of IL-26 in human gastric cancer (GC). The expression of IL-26 and related molecules such as IL-20R1, STAT1 and STAT3 was examined by real-time PCR and immunohistochemisty. The effects of IL-26 on cell proliferation and cisplatin-induced apoptosis were analyzed by BrdU cooperation assay and PI-Annexin V co-staining, respectively. Lentiviral mediated siRNA was used to explore its mechanism of action, and IL-26 related signaling was analyzed by western blotting. Human GC tissues showed increased levels of IL-26 and its related molecules and activation of STAT3 signaling, whereas STAT1 activation did not differ significantly between GC and normal gastric tissues. Moreover, IL-26 was primarily produced by Th17 and NK cells. IL-26 promoted the proliferation and survival of MKN45 and SGC-7901 gastric cancer cells in a dose-dependent manner. Furthermore, IL-20R2 and IL-10R1, which are two essential receptors for IL-26 signaling, were expressed in both cell lines. IL-26 activated STAT1 and STAT3 signaling; however, the upregulation of the expression of Bcl-2, Bcl-xl and c-myc indicated that the effect of IL-26 is mediated by STAT3 activation. Knockdown of STAT1 and STAT3 expression suggested that the proliferative and anti-apoptotic effects of IL-26 are mediated by the modulation of STAT1/STAT3 activation. In summary, elevated levels of IL-26 in human GC promote proliferation and survival by modulating STAT1/STAT3 signaling

Hollow fiber polytetrafluoroethylene membrane heat exchanger with anti-corrosion properties

Hollow fiber polytetrafluoroethylene membrane heat exchanger with anti-corrosion propertie