Study on the Iodine 125 Uptake of H460 Lung Cancer Cell Line by Co-transfection with the Human Sodium/Iodide Symporter and the Human Thyroperoxidase

Background and objective Lung cancer harms people’s health or even lives severely. Especially, the therapy of non-small cell lung cancer (NSCLC) has not been obviously improved for many years. The aim of this study is to transfer the human sodium/iodide symporter (hNIS) and the human thyroperoxidase (hTPO) genes into H460 lung cancer cell line, and to study the uptake ability of iodide after co-transfected hTPO and hNIS gene in cell lines. Methods Through cloning, recombination, packaging and amplifying, the recombinant adenosine virus (AdTPO) was constructed. Then the protein expression of AdTPO was tested by Western blot. After transfected hNIS gene into human lung cancer cell line H460 through liposome, stably expressing hNIS gene cell lines (hNIS-H460) selected by G418 antibiotics was determined as hNIS-H460 group. Using AdTPO, hTPO gene was transducted into hNIS-H460, as AdTPO-hNIS-H460 group. H460 cell without hNIS gene was applied as control group (H460). Then, we investigated the 125I uptake assay of the above cells. Results We were successful in co-transfecting hNIS and hTPO gene into human lung cell lines H460, and were obtained hNIS and hTPO gene lung cancer cell lines (hNIS-H460 and AdTPO-hNIS-H460). In AdTPO-hNIS-H460, hNIS-H460 and H460, the uptake ability of 125I was (59 637.67±1 281.13), (48 622.17±2 242.28) and (1 440.17±372.86) counts•min-1. The uptake ability of 125I was 41 fold higher in AdTPO-hNIS-H460 than in blank control H460 (P<0.01), and 34 fold higher in hNIS-460 than in blank control H460 (P<0.01), and 1.2 fold higher in AdTPO-hNIS-H460 than in hNIS-H460 (P<0.01). Conclusion The uptake ability of 125I could increase by co-transfected hNIS and hTPO genes into human lung cancer cell lines H460

TREATMENT AND MODELING OF EPSTEIN-BARR VIRUS POST-TRANSPLANT LYMPHOPROLIFERATIVE DISORDER

Ph.DDOCTOR OF PHILOSOPH

Solving the Maximum Independent Set Problem based on Molecule Parallel Supercomputing

The maximum independent set Problem is to find a biggest vertex independent set in a given undirected graph. It is a vitally important NP problem in graph theory and applied mathematics, having numerous real life applications. It can be difficultly solved by the electronic computer in exponential level time. Simultaneity in previous studies DNA molecular computation usually be used to solve NP-complete continuous path search problems (for example HPP, traveling salesman problem), rarely for NP problems with discrete vertex or path solutions result, such as the maximum independent set problem, graph coloring problem and so on. In this paper, we present a new algorithm for solving the maximum independent set problem with DNA molecular operations. For an undirected graph with n vertices, We reasonably design fixed length DNA strands representing the vertices and edges of graph, take appropriate steps and get the solutions of the problem in proper length range using O(n2) time. We extend the application of DNA molecular operations and simultaneity simplify the complexity of the computation

The theoretical direct-band-gap optical gain of Germanium nanowires

We calculate the electronic structures of Germanium nanowires by taking the effective-mass theory. The electron and hole states at the G-valley are studied via the eight-band k.p theory. For the [111] L-valley, we expand the envelope wave function using Bessel functions to calculate the energies of the electron states for the first time. The results show that the energy dispersion curves of electron states at the L-valley are almost parabolic irrespective of the radius of Germanium nanowires. Based on the electronic structures, the density of states of Germanium nanowires are also obtained, and we find that the conduction band density of states mostly come from the electron states at the L-valley because of the eight equivalent degenerate L points in Germanium. Furthermore, the optical gain spectra of Germanium nanowires are investigated. The calculations show that there are no optical gain along z direction even though the injected carrier density is 4x1019 cm-3 when the doping concentration is zero, and a remarkable optical gain can be obtained when the injected carrier density is close to 1x1020 cm-3, since a large amount of electrons will prefer to occupy the low-energy L-valley. In this case, the negative optical gain will be encountered considering free-carrier absorption loss as the increase of the diameter. We also investigate the optical gain along z direction as functions of the doping concentration and injected carrier density for the doped Germanium nanowires. When taking into account free-carrier absorption loss, the calculated results show that a positive net peak gain is most likely to occur in the heavily doped nanowires with smaller diameters. Our theoretical studies are valuable in providing a guidance for the applications of Germanium nanowires in the field of microelectronics and optoelectronics

Augmented 2D-TAN: A Two-stage Approach for Human-centric Spatio-Temporal Video Grounding

We propose an effective two-stage approach to tackle the problem of language-based Human-centric Spatio-Temporal Video Grounding (HC-STVG) task. In the first stage, we propose an Augmented 2D Temporal Adjacent Network (Augmented 2D-TAN) to temporally ground the target moment corresponding to the given description. Primarily, we improve the original 2D-TAN from two aspects: First, a temporal context-aware Bi-LSTM Aggregation Module is developed to aggregate clip-level representations, replacing the original max-pooling. Second, we propose to employ Random Concatenation Augmentation (RCA) mechanism during the training phase. In the second stage, we use pretrained MDETR model to generate per-frame bounding boxes via language query, and design a set of hand-crafted rules to select the best matching bounding box outputted by MDETR for each frame within the grounded moment.Comment: Best Paper Award at the 3rd Person in Context (PIC) Challenge CVPR Workshop 202

Effectiveness of a Four-days/Eight Hour Work Week

This research paper explores the benefits of a four-day workweek that may affect productivity, self-satisfaction, and life expectancy. We will be using data on a country-wide scale from 25 first world countries over four continents. We will perform regression analysis to investigate the impact of a four-day workweek. The data will be collected using free and public sources published online like Our World In Data and Statista, which include GDP per capita, life satisfaction rate, human development index, and annual working hours. The results of our research and analysis will show how each independent variable will affect the dependent variable of our study, as well as any correlation between them. We aim to uncover any benefits of switching to a four day/eight-hour workweek and support the move for companies to reconsider the traditional five day/eight-hour workweek in hopes of improving the quality of life of working adults. Our results show that Human Development Index, Gross Domestic Product has a p-value of more than 0.05, and therefore we do not reject the null hypothesis that the four days work week has no impact on productivity in the workforce. Life Satisfaction Rate on the other hand has a p-value of less than 0.05, thus rejecting the null hypothesis that the four days work week has no impact on the satisfaction rate of the workforce