58 research outputs found
AN OXANTHROQUINONE DERIVATIVE DISRUPTS RAS PLASMA MEMBRANE LOCALIZATION AND FUNCTION BY INHIBITION OF ACYLPEPTIDE HYDROLASE AND PERTURBATION OF SPHINGOMYELIN METABOLISM
Oncogenic RAS proteins are commonly expressed in human cancer. To be functional, RAS proteins must undergo post-translational modification and localize to the plasma membrane (PM). Therefore, compounds that prevent RAS PM targeting have potential as putative RAS inhibitors. Here we examined the mechanism of action of oxanthroquinone G01 (G01), a recently described inhibitor of KRAS PM localization. We show that G01 mislocalized HRAS and KRAS from the PM with similar potency and disrupted the spatial organization of RAS proteins remaining on the PM. G01 also inhibited recycling of epidermal growth factor receptor and transferrin receptor, but did not impair internalization of cholera toxin, indicating suppression of recycling endosome function. In searching for the mechanism of impaired endosomal recycling we observed that G01 also enhanced cellular sphingomyelin (SM) and ceramide levels and disrupted the localization of several lipid and cholesterol reporters, suggesting that the G01 molecular target may involve SM metabolism. Indeed, G01 exhibited potent synergy with other compounds that target SM metabolism in KRAS localization assays.
When attached to a biotin moiety, G01 bound acylpeptide hydrolase (APEH) and blocked its enzymatic activity. Inhibition of APEH by ebelactone, an esterase inhibitor, or the knockdown of APEH mislocalized KRASG12V as well as phosphatidylserine from the PM. Together, these results suggest that G01 mislocalizes KRASG12V from the PM by binding to and inhibiting APEH function.
Furthermore, G01 significantly abrogated RAS-RAF-MAPK signaling in MDCK cells expressing constitutively activated, oncogenic mutant RASG12V. G01 also inhibited the proliferation of RAS-less mouse embryonic fibroblasts (MEFs) expressing oncogenic mutant KRASG12V or KRASG12D but not RAS-less MEFs expressing oncogenic mutant BRAFV600E. Consistent with these effects, G01 inhibited the proliferation of KRAS-transformed pancreatic, colon, and endometrial cancer cells. Taken together, these results suggest that G01 should undergo further evaluation as a potential anti-RAS therapeutic.
To enhance the potency of G01 for KRAS inhibition, we synthesized D01 and D02, two derivatives of G01 containing an additional geranyl or farnesyl group, respectively. D01 or D02 mislocalized KRAS more effectively than G01, and disrupted the clustering of KRAS at a much lower concentration than G01. As a consequence, D01 or D02 inhibited MAPK signaling downstream of oncogenic KRAS, as well as the proliferation of KRAS-dependent pancreatic cancer cells
Coupled Deep Learning for Heterogeneous Face Recognition
Heterogeneous face matching is a challenge issue in face recognition due to
large domain difference as well as insufficient pairwise images in different
modalities during training. This paper proposes a coupled deep learning (CDL)
approach for the heterogeneous face matching. CDL seeks a shared feature space
in which the heterogeneous face matching problem can be approximately treated
as a homogeneous face matching problem. The objective function of CDL mainly
includes two parts. The first part contains a trace norm and a block-diagonal
prior as relevance constraints, which not only make unpaired images from
multiple modalities be clustered and correlated, but also regularize the
parameters to alleviate overfitting. An approximate variational formulation is
introduced to deal with the difficulties of optimizing low-rank constraint
directly. The second part contains a cross modal ranking among triplet domain
specific images to maximize the margin for different identities and increase
data for a small amount of training samples. Besides, an alternating
minimization method is employed to iteratively update the parameters of CDL.
Experimental results show that CDL achieves better performance on the
challenging CASIA NIR-VIS 2.0 face recognition database, the IIIT-D Sketch
database, the CUHK Face Sketch (CUFS), and the CUHK Face Sketch FERET (CUFSF),
which significantly outperforms state-of-the-art heterogeneous face recognition
methods.Comment: AAAI 201
Correlation of Circulating Glucocorticoid-Induced TNFR-Related Protein Ligand Levels with Disease Activity in Patients with Systemic Lupus Erythematosus
The aim of this paper is to investigate the correlation of glucocorticoid-induced tumor necrosis factor receptor- (TNFR-) related protein ligand (GITRL) with disease activity and organ involvement in patients with systemic lupus erythematosus (SLE). Serum GITRL levels were measured in 58 patients with SLE and 30 healthy controls matched for age and sex. Patients were assessed for clinical and laboratory variables. Correlations of serum GITRL levels with SLEDAI, laboratory values, and clinical manifestations were assessed. Serum GITRL levels were determined by ELISA. Serum GITRL levels were markedly increased in patients with SLE compared with healthy controls (mean 401.3 ng/mL and 36.59 ng/mL, resp.; P<0.0001). SLE patients with active disease showed higher serum GITRL levels compared to those with inactive disease (mean 403.3 ng/mL and 136.3 ng/mL, resp; P=0.0043) as well as normal controls (36.59 ng/mL; P<0.0001). Serum GITRL levels were positively correlated with SLEDAI, titers of anti-dsDNA antibody, erythrocyte sedimentation rate (ESR), and IgM and negatively correlated with complement3 (C3). Serum GITRL levels were higher in SLE patients with renal involvement and vasculitis compared with patients without the above-mentioned manifestations
Leveraging automated privacy checking for design of mobile privacy protection mechanisms
National Research Foundation (NRF) Singapor
Graph-aided directed testing of Android applications for checking runtime privacy behaviours
National Research Foundation (NRF) Singapore under Interactive Digital Media Strategic Research Programm
- …