A specific type of cyclin-like F-box domain gene is involved in the cryogenic autolysis of \u3ci\u3eVolvariella volvacea\u3c/i\u3e

Cryogenic autolysis is a typical phenomenon of abnormal metabolism in Volvariella volvacea. Recent studies have identified 20 significantly upregulated genes via high-throughput sequencing of the mRNAs expressed in the mycelia of V. volvacea after cold exposure. Among these significantly upregulated genes, 15 annotated genes were used for functional annotation cluster analysis. Our results showed that the cyclin-like F-box domain (FBDC) formed the functional cluster with the lowest P-value. We also observed a significant expansion of FBDC families in V. volvacea. Among these, the FBDC3 family displayed the maximal gene expansion in V. volvacea. Gene expression profiling analysis revealed only one FBDC gene in V. volvacea (FBDV1) that was significantly up-regulated, which is located in the FBDC3 family. Comparative genomics analysis revealed the homologous sequences of FBDV1 with high similarity were clustered on the same scaffold. However, FBDV1 was located far from these clusters, indicating the divergence of duplicated genes. Relative time estimation and rate test provided evidence for the divergence of FBDV1 after recent duplications. Real-time RT-PCR analysis confirmed that the expression of the FBDV1 was significantly up-regulated (P , 0.001) after cold-treatment of V. volvacea for 4 h. These observations suggest that the FBDV1 is involved in the cryogenic autolysis of V. volvacea

Radiative thermal switch via metamaterials made of vanadium dioxide-coated nanoparticles

In this work, a thermal switch is proposed based on the phase-change material vanadium dioxide (VO2) within the framework of near-field radiative heat transfer (NFRHT). The radiative thermal switch consists of two metamaterials filled with core-shell nanoparticles, with the shell made of VO2. Compared to traditional VO2 slabs, the proposed switch exhibits a more than 2-times increase in the switching ratio, reaching as high as 90.29% with a 100 nm vacuum gap. The improved switching effect is attributed to the capability of the VO2 shell to couple with the core, greatly enhancing heat transfer with the insulating VO2, while blocking the motivation of the core in the metallic state of VO2. As a result, this efficiently enlarges the difference in photonic characteristics between the insulating and metallic states of the structure, thereby improving the ability to rectify the NFRHT. The proposed switch opens pathways for active control of NFRHT and holds practical significance for developing thermal photon-based logic circuits

Dual Effects of Chinese Herbal Medicines on Angiogenesis in Cancer and Ischemic Stroke Treatments: Role of HIF-1 Network

This work is licensed under a Creative Commons Attribution 4.0 International License.Hypoxia-inducible factor-1 (HIF-1)–induced angiogenesis has been involved in numerous pathological conditions, and it may be harmful or beneficial depending on the types of diseases. Exploration on angiogenesis has sparked hopes in providing novel therapeutic approaches on multiple diseases with high mortality rates, such as cancer and ischemic stroke. The HIF-1 pathway is considered to be a major regulator of angiogenesis. HIF-1 seems to be involved in the vascular formation process by synergistic correlations with other proangiogenic factors in cancer and cerebrovascular disease. The regulation of HIF-1–dependent angiogenesis is related to the modulation of HIF-1 bioactivity by regulating HIF-1α transcription or protein translation, HIF-1α DNA binding, HIF-1α and HIF-1α dimerization, and HIF-1 degradation. Traditional Chinese herbal medicines have a long history of clinical use in both cancer and stroke treatments in Asia. Growing evidence has demonstrated potential proangiogenic benefits of Chinese herbal medicines in ischemic stroke, whereas tumor angiogenesis could be inhibited by the active components in Chinese herbal medicines. The objective of this review is to provide comprehensive insight on the effects of Chinese herbal medicines on angiogenesis by regulating HIF-1 pathways in both cancer and ischemic stroke.National Natural Science Foundation of China (No. 81673627)Guangzhou Science Technology and Innovation Commission Research Projects (201805010005)Research Grant Council, HKSAR (Project code: RGC GRF 17152116)Commissioner for Innovation Technology, HKSAR (Project code: ITS/091/16FX

Pluripotency factors functionally premark cell-type-restricted enhancers in ES cells.

Enhancers for embryonic stem (ES) cell-expressed genes and lineage-determining factors are characterized by conventional marks of enhancer activation in ES cells1-3, but it remains unclear whether enhancers destined to regulate cell-type-restricted transcription units might also have distinct signatures in ES cells. Here we show that cell-type-restricted enhancers are 'premarked' and activated as transcription units by the binding of one or two ES cell transcription factors, although they do not exhibit traditional enhancer epigenetic marks in ES cells, thus uncovering the initial temporal origins of cell-type-restricted enhancers. This premarking is required for future cell-type-restricted enhancer activity in the differentiated cells, with the strength of the ES cell signature being functionally important for the subsequent robustness of cell-type-restricted enhancer activation. We have experimentally validated this model in macrophage-restricted enhancers and neural precursor cell (NPC)-restricted enhancers using ES cell-derived macrophages or NPCs, edited to contain specific ES cell transcription factor motif deletions. DNA hydroxyl-methylation of enhancers in ES cells, determined by ES cell transcription factors, may serve as a potential molecular memory for subsequent enhancer activation in mature macrophages. These findings suggest that the massive repertoire of cell-type-restricted enhancers are essentially hierarchically and obligatorily premarked by binding of a defining ES cell transcription factor in ES cells, dictating the robustness of enhancer activation in mature cells

Retrieval of Optical Constant and Particle Size Distribution of Particulate Media Using the PSO-Based Neural Network Algorithm

An improved neural network algorithm was proposed and applied to the inverse radiative problems. A multi-strategy particle swarm optimization was applied to improve the performance of the back propagation multi-layer feed-forward neural network algorithm. Three commonly used particle size distribution (PSD) functions in a one-dimensional particle system were retrieved using the proposed algorithm. In addition, the optical constant was also estimated, and the measurement errors were considered. Results show that the proposed algorithm can be applied to the retrieval of PSDs and optical constant even with measurement errors. Finally, the proposed algorithm was applied to the simultaneous estimation of the PSDs and optical constant using the multi-wavelength and multi-thickness method

Geometric calibration of focused light field camera for 3-D flame temperature measurement

Focused light field camera can be used to measure three-dimensional (3-D) temperature field of a flame because of its ability to record intensity and direction information of each ray from flame simultaneously. This work aims to develop a suitable geometric calibration method of focused light field camera for 3-D flame temperature measurement. A modified method based on Zhang's camera calibration is developed to calibrate the camera and the measurement system. A single focused light-field camera is used to capture images of bespoke calibration board for calibration in this study. Geometric parameters including intrinsic (i.e., camera parameters) and extrinsic (i.e., camera connecting with the calibration board) of the focused light field camera are calibrated to trace the ray projecting onto each pixel on CCD (charge-coupled device) sensor. Instead of using line features, corner point features are directly utilized for the calibration. The characteristics of focused light field camera including one 3-D point corresponding to several image points and matching main lens and microlens f-numbers, are used for calibration. Results with a focused light field camera are presented and discussed. Preliminary 3-D temperature distribution of a flame is also investigated and presented

Multiple magnetoplasmon polaritons of magneto-optical graphene in near-field radiative heat transfer

Graphene, as a two-dimensional magneto-optical material, supports magnetoplasmon polaritons (MPP) when exposed to an applied magnetic field. Recently, MPP of a single-layer graphene has shown an excellent capability in the modulation of near-field radiative heat transfer (NFRHT). In this study, we present a comprehensive theoretical analysis of NFRHT between two multilayered graphene structures, with a particular focus on the multiple MPP effect. We reveal the physical mechanism and evolution law of the multiple MPP, and we demonstrate that the multiple MPP allow one to mediate, enhance, and tune the NFRHT by appropriately engineering the properties of graphene, the number of graphene sheets, the intensity of magnetic fields, as well as the geometric structure of systems. We show that the multiple MPP have a quite significant distinction relative to the single MPP or multiple surface plasmon polaritons (SPPs) in terms of modulating and manipulating NFRHT

Performance improvement of three-body radiative diode driven by graphene surface plasmon polaritons

As an analogue to electrical diode, a radiative thermal diode allows radiation to transfer more efficiently in one direction than in the opposite direction by operating in a contactless mode. In this study, we demonstrated that, within the framework of three-body photon thermal tunneling, the rectification performance of three-body radiative diode can be greatly improved by bringing graphene into the system. The system is composed of three parallel slabs, with the hot and cold terminals of the diode coated with graphene films, and the intermediate body made of vanadium dioxide (VO2). The rectification factor of the proposed radiative thermal diode reaches 300 % with a 350 nm separation distance between the hot and cold terminals of the diode. With the help of graphene, the rectification performance of the radiative thermal diode can be improved by over 11 times. By analyzing the spectral heat flux and energy transmission coefficients, it was found that the improved performance is primarily attributed to the surface plasmon polaritons (SPPs) of graphene. They excite the modes of insulating VO2 in the forward-biased scenario by forming strongly coupled modes between graphene and VO2, and thus dramatically enhance the heat flux. While, for the reverse-biased scenario, the VO2 is at its metallic state and thus graphene SPPs cannot work by three-body photon thermal tunneling. Furthermore, the improvement was also investigated for different chemical potentials of graphene, and geometric parameters of the three-body system. Our findings demonstrate the feasibility of using thermal-photon-based logical circuits, creating radiation-based communication technology, and implementing thermal management approaches at the nanoscale

Inverse Geometry Design of Radiative Enclosures Using Particle Swarm Optimization Algorithms

Three different Particle Swarm Optimization (PSO) algorithms—standard PSO, stochastic PSO (SPSO) and differential evolution PSO (DEPSO)—are applied to solve the inverse geometry design problems of radiative enclosures. The design purpose is to satisfy a uniform distribution of radiative heat flux on the designed surface. The design surface is discretized into a series of control points, the PSO algorithms are used to optimize the locations of these points and the Akima cubic interpolation is utilized to approximate the changing boundary shape. The retrieval results show that PSO algorithms can be successfully applied to solve inverse geometry design problems and SPSO achieves the best performance on computational time. The influences of the number of control points and the radiative properties of the media on the retrieval geometry design results are also investigated