Vegetation outlines of two active rock glaciers with contrasting lithology

Rock glaciers are periglacial landforms consisting of coarse debris with interstitial ice or ice core, characterized by creeping due to ice deformation. These landforms are drawing the attention of plant ecologist as harsh habitats and potential refugia in the global change context. Our aim was to describe the vegetation outlines of two active rock glaciers of the Ortles-Cevedale Massif (Central Italian Alps) on different substrates (silicate and carbonate) and compare them with the neighboring stable slopes and scree slopes. Two hypotheses were tested: 1) rock glaciers differ from the surrounding landforms for the presence of cold-adapted plant communities; 2) rock glacier plant communities indicate similar microclimatic conditions in spite of the contrasting lithology. Data were collected by phytosociological method performing 80 relev\ue9s of 25 m\ub2. Plant communities were compared by a cluster analysis based on the presence/absence species matrix and species relative frequencies for each landform were calculated. The cluster analysis separated first for all the two sites; afterwards, the landforms were differently discerned each other depending on the site. Despite the remarkable floristic differences due to the substrate, the vegetation of both rock glaciers suggest a general adjustment to cold-moist microclimate and long-lasting snow cover, differentiating more or less evidently from the adjacent scree slopes and enhancing the survival of nival entities at the elevation of alpine grasslands

Combination of nanomicellar technology and in situ gelling polymer as ocular drug delivery system (Odds) for cyclosporine-a

A combination of in situ gelling systems and a loaded drug self-assembling nanomicellar carrier was chosen in this study as a new potential Ocular Drug Delivery System (ODDS) for Cy-closporine-A (CyA), a poorly water-soluble drug. Two non-ionic surfactants (d-α-tocopherol polyethylene glycol succinate, VitE-TPGS and polyoxyl 40 hydrogenated castor oil, RH-40) were used to produce the nanomicelles. The physical–chemical characterization of the nanomicelles in terms of CyA entrapment (EE%) and loading efficiency (LE%), cloud point (CP), regeneration time (RT), size and polydispersity index (PI) allowed us to select the best combination of surfactant mixture, which showed appropriate stability, high CyA-EE (99.07%), very small and homogeneous dimen-sions and favored the solubilization of an amount of CyA (0.144% w/w) comparable to that con-tained in marketed emulsion Ikervis®. The selected nanomicellar formulation incorporated into optimized ion-sensitive polymeric dispersions of gellan gum (GG-LA: 0.10, 0.15 and 0.20% w/w) able to trigger the sol–gel transition after instillation was characterized from technological (osmo-lality, pH, gelling capacity, rheological behavior, wettability, TEM and storage stability at 4 and 20 °C) and biopharmaceutical points of view. This new combined approach allowed us to obtain clear aqueous dispersions that were easy to instill and able to form a viscous gel when in contact with the tear fluid, improving CyA ocular bioavailability. Furthermore, this new ODDS prevented CyA transcorneal permeation, exhibited low cytotoxicity and prolonged the CyA resident time in the precorneal area compared to Ikervis®

Hydrogels as Corneal Stroma Substitutes for In Vitro Evaluation of Drug Ocular Permeation

Hydrogels are complex hydrophilic structures, consisting of crosslinked homopolymers or copolymers insoluble in water. Due to their controllable bio-physicochemical properties mimicking the morphology of the native extracellular matrix, they are a key part of a lot of research fields, including medicine, pharmaceutics, and tissue engineering. This paper was focused on the preparation and characterization of hydrogels from different blends of polyvinyl alcohol (PVA) with microcrystalline cellulose (MCC) and gelatin (GEL) at various ratios, and from gelatin and chitosan alone to understand their feasibility of utilizing as corneal stroma substitutes in permeability tests for drug candidate molecules in early stages of their development. The characterization was carried out by differential scanning calorimetry, electron microscopy (SEM), water content, mass loss, water permeability, wettability, and tensile stress-strain tests. After the physicochemical characterization, PVA/MCC blend and chitosan proved to be the most promising constructs, showing negligible mass loss after immersion in aqueous medium for two weeks and low hydrodynamic permeability. They were then employed in drug molecules permeation studies and these data were compared to that obtained through excised tissues. The results obtained showed that PVA/MCC hydrogels have similar mechanical and permeability properties to corneal stroma

Influence of a Combination of Chemical Enhancers and Iontophoresis on In Vitro Transungual Permeation of Nystatin

To promote transungual permeation of nystatin (NYST), molecule with high molecular weight, no water-soluble, amphoteric by iontophoresis. The synergic effect of the combination of cetylpyridinium chloride, CPC, or polyoxyethylene (20) sorbitan monooleate, TW80, and iontophoresis was investigated. In vitro permeation experiments were carried out through bovine hoof slices using vertical diffusion cells. A low current density (0.2 mA/cm2) was applied by introducing Ag/AgCl electrodes in the donor (anode) and receptor (cathode) chambers. The donor phase consisted of a solution, a suspension, or gel-type vehicles containing NYST and surfactants in pH 5.6 HEPES buffer. The addition of CPC to NYST suspension (SOSP) produced a fivefold increase on the permeability of the bovine hoof membrane to the drug. The application of anodal iontophoresis further improved NYST flux. Conversely, NYST transungual permeation was not influenced by TW80 either in the passive diffusion or iontophoretic flux. Furthermore, the iontophoretic treatment does not appear to induce irreversible alterations to the hoof bovine membranes. The present work demonstrated the efficacy of iontophoresis as a treatment for different nail pathologies with large molecules very slightly soluble in water without irreversibly affecting the nail structure. A synergistic effect between CPC and iontophoresis was observed

Thesaurus: un database per il patrimonio culturale sommerso

Thesaurus Project aims at promoting the knowledge of the underwater cultural heritage, ancient and modern, through the application of several typologies of tools: underwater autonomous vehicles, which will be able to explore the sea bottom in teams communicating with each other; a database, which will be useful to store and manage all the information referring to archaeological or historical objects, shipwrecks and sites. This paper aims to explain the logic structure of the database indicating the particular needs of the research, the different typologies of items which have to be managed (archaeological and historical objects; ancient, medieval or modern shipwrecks; underwater sites; written or figurative sources, etc.), the relation with other similar databases and projects. The main task of this part of Thesaurus is to plan and organize an IT system, which will allow archaeologists to describe information in detail, in order to make an efficient managing and retrieving data system available

Assembling surfactants-mucoadhesive polymer nanomicelles (ASMP-nano) for ocular delivery of cyclosporine-A

The physiological protective mechanisms of the eye reduce the bioavailability of topically administered drugs above all for those with high molecular weight and /or lipophilic characteristics, such as Cyclosporine A (CyA). The combined strategy based on the association of nanomicelles and mucoadhesive polymer seems promising since a limited number of commercial products containing CyA have been recently approved. The scope of this investigation was the design of Assembling Surfactants-Mucoadhesive Polymer Nanomicelles (ASMP-Nano), based on a binary system of two surfactants in combination with hyaluronic acid, and their biopharmaceutical evaluation. The optimisation of the ASMP-Nano in term of the amount of surfactants, CyA-loading and size determined the selection of the clear and stable Nano1HAB-CyA formulation containing 0.105% w/w CyA loaded-nanomicelles with a size of 14.41 nm. The nanostructured system had a protective effect towards epithelial corneal cells with a cell viability of more than 80%. It interacted with cellular barriers favouring the uptake and the accumulation of CyA into the cells as evidenced by fluorescent probe distribution, by hindering CyA permeation through reconstituted corneal epithelial tissue. In pharmacokinetics study on rabbits, the nanomicellar carrier prolonged the CyA retention time in the precorneal area mainly in presence of hyaluronic acid (HA), a mucoadhesive polymer

Life in harsh environments : carabid and spider trait types and functional diversity on a debris-covered glacier and along its foreland

1. Patterns of species richness and species assemblage composition of ground-dwelling arthropods in primary successions along glacier forelands are traditionally described using a taxonomic approach. On the other hand, the functional trait approach could ensure a better characterisation of their colonisation strategies in these types of habitat. 2. The functional trait approach was applied to investigate patterns of functional diversity and life-history traits of ground beetles and spiders on an alpine debris-covered glacier and along its forefield in order to describe their colonisation strategies. 3. Ground beetles and spiders were sampled at different successional stages, representing five stages of deglaciation. 4. The results show that the studied glacier hosts ground beetle and spider assemblages that are mainly characterised by the following traits: walking colonisers, ground hunters and small-sized species. These traits are typical of species living in cold, wet, and gravelly habitats. The diversity of functional traits in spiders increased along the succession, and in both carabids and spiders, life-history traits follow the \u2018addition and persistence model\u2019. Accordingly, there is no turnover but there is an addition of new traits and a variation in their proportion within each species assemblage along the succession. The distribution of ground beetles and spiders along the glacier foreland and on the glacier seems to be driven by dispersal ability and foraging strategy. 5. The proposed functional approach improves knowledge of the adaptive strategies of ground-dwelling arthropods colonising glacier surfaces and recently deglaciated terrains, which represent landforms quickly changing due to global warming

Tyrosol-Enriched Tomatoes by Diffusion across the Fruit Peel from a Chitosan Coating: A Proposal of Functional Food

Chitosan is receiving increasing attention from the food industry for being a biodegradable, non-toxic, antimicrobial biopolymer able to extend the shelf life of, and preserve the quality of, fresh food. However, few studies have investigated the ability of chitosan-based coatings to allow the diffusion of bioactive compounds into the food matrix to improve its nutraceutical quality. This research is aimed at testing whether a hydrophilic molecule (tyrosol) could diffuse from the chitosan-tyrosol coating and cross the tomato peel. To this end, in vitro permeation tests using excised tomato peel and an in vivo application of chitosan-tyrosol coating on tomato fruit, followed by tyrosol quantification in intact fruit, peel and flesh during a seven-day storage at room temperature, were performed. Both approaches demonstrated the ability of tyrosol to permeate across the fruit peel. Along with a decreased tyrosol content in the peel, its concentration within the flesh was increased, indicating an active transfer of tyrosol into this tissue. This finding, together with the maintenance of constant tyrosol levels during the seven-day storage period, is very promising for the use of chitosan formulations to produce functional tomato fruit

pH-responsive nanostructures based on surface active fatty acid-protic ionic liquids for imiquimod delivery in skin cancer topical therapy

or topical treatment of skin cancer, the design of pH-responsive nanocarriers able to selectively release the drug in the tumor acidic microenvironment represents a reliable option for targeted delivery. In this context, a series of newly synthesized surface-active fatty acid-protic ionic liquids (FA-PILs), based on tetramethylguanidinium cation and different natural hydrophobic fatty acid carboxylates, have been investigated with the aim of developing a pH-sensitive nanostructured drug delivery system for cutaneous administration in the skin cancer therapy. The capability of FA-PILs to arrange in micelles when combined with each other and with the non-ionic surfactant d-α-Tocopherol polyethylene glycol succinate (vitamin E TPGS) as well as their ability to solubilize imiquimod, an immuno-stimulant drug used for the treatment of skin cancerous lesions, have been demonstrated. The FA-PILs-TPGS mixed micelles showed pH-sensitivity, suggesting that the acidic environment of cancer cells can trigger nanostructures’ swelling and collapse with consequent rapid release of imiquimod and drug cytotoxic potential enhancement. The in vitro permeation/penetration study showed that the micellar formulation produced effective imiquimod concentrations into the skin exposed to acid environment, representing a potential efficacious and selective drug delivery system able to trigger the drug release in the tumor tissues, at lower and less irritating drug concentrations. © 2020 by the author

Glacial and periglacial landforms as warm-stage refugia for cold-adapted plant and arthropod species:ecological and biogeographical significance of a habitat system

Since 1880 air temperature at global scale rapidly increased of c. 0.85 \ub0C likely due to the increasing amount of anthropogenic greenhouse gas, and is forecast to increase over the 21st century as well. Cold-adapted species of the high latitude/altitude are the first to be threatened by such climate change, due to the progressive reduction of their suitable areas. Microclimatic heterogeneity at landscape level is supposed to play a key role in this scenario providing warm-stage refugia, sites able to preserve suitable climate conditions for cold-adapted species in spite of the ongoing climate warming. Active rock glaciers and debris-covered glaciers (periglacial and glacial landforms characterized by debris with underlying ice) were proposed as potential warm-stage refugia in mountain regions, due to the microclimate effect of underlying ice and the thermal inertia provided by the debris fraction. Besides microclimate heterogeneity at landscape level, macroclimate heterogeneity at regional scale (e.g. the contrast between continental and oceanic climates of inner and peripheral mountain ranges, respectively) can be important, since drive the distribution of periglacial and glacial landforms as potential warm-stage refugia and the effects of climate change themselves on high mountain landscapes (e.g. temporal pattern of glaciers retreat) and ecosystems (e.g. temporal pattern of primary successions on ice-free glacial landforms). Aim of this study was to analyze the ecological features of periglacial and glacial landforms (active rock glaciers and debris-covered glaciers on inner mountain ranges, glacier forelands and recent moraines on peripheral mountain ranges) to test whether they have the main key-requirements to act as potential warm-stage refugia for cold-adapted species. Concerning active rock glaciers and debris-covered glaciers, we considered as key-requirements a cold microclimate able to support cold-adapted species and the ability to cross the altitudinal zonation of mountain ecosystems, thus supporting cold-adapted species in adverse climate contexts. Concerning glacial landforms on peripheral mountain ranges, we considered as key-requirement a relatively slow colonization pattern able to long-lasting support pioneer cold-adapted species in spite of the primary succession development. The study was carried out in different areas of the Italian Alps (Adamello-Presanella, Ortles-Cevedale, Monte Rosa, Orobian Alps) in which different landforms were analyzed and compared. A multidisciplinary approach was followed, considering both the abiotic (ground surface temperature and humidity, soil physical and chemical parameters) and biotic variables (plant and arthropod communities). Our results show as active rock glaciers and debris-covered glaciers does not differ from the surrounding debris-featured iceless landforms for soil parameters, but for a cold microclimate likely due to the underlying ice. The microclimate can explain why these landforms differ from the surrounding ones for the occurrence of cold-adapted plant and arthropod species. On the Alps, active rock glaciers are unable to cross the altitudinal zonation of mountain ecosystems, thus can only enhance the occurrence of cold-adapted species within their normal altitudinal distribution. By contrast, debris-covered glaciers can cross the climatic treeline, allowing cold-adapted species to live even below their normal altitudinal distribution. However, the relative altitudinal distribution of active rock glaciers and debris-covered glaciers can be inverted on mountain systems drier than Alps, suggesting a similar ecological role of such landforms in opposite macroclimate conditions. Moreover, our results suggest that mountain systems characterized by oceanic macroclimate could prevent the extinction risk for pioneer cold-adapted species along a chronosequence of glacier retreat, due to the long-lasting persistence of glaciers at relatively low altitudes (as a consequence of high winter precipitations) and the slow colonization of ice-free glacial landforms (as a consequence of long-lasting snow cover and temperatures around zero). Peripheral mountain ranges in particular outlines as oceanic areas of crucial biogeographical importance during both the opposite climate extremes: in cold-climatic stages they can provide refugia for a lot of species, as a consequence of the relatively low ice-sheet expansion; in warm-climatic stages they may provide refugia for cold-adapted species in particular, as a consequence of their climatic, thus glaciological and ecological features