3 research outputs found
Variants of ESR1, APOE, LPL and IL-6 loci in young healthy subjects: association with lipid status and obesity
Findings BMI was increased (>25) in 22% of young healthy subjects. Increased cholesterol values (>5.0 mmol/L) were found in 23% of subjects, LDL-C (>3.0 mmol/L) in 23%, triglycerides (>1.7 mmol/L) in 11% of subjects. We found statistically significant differences in subjects' weight (p = 0.015), BMI (p = 0.023), and waist-hip ratio (WHR) (p = 0.015) in regard to their diet type; subjects with Mediterranean diet had the lowest values compared to those on continental and mixed diet. Significant associations were found for: LPL genetic polymorphic variant and abdominal obesity (p = 0.013), APO epsilon4 allele and hypercholesterolemia (p = 0.003), and ESR1-TA long allele and hypercholesterolemia (p = 0.011). ----- Background Human obesity is a multifactorial syndrome influenced also by genetic factors. Among gene variants found to be involved in body weight regulation and development of obesity, particular attention has been paid to polymorphisms in genes associated with obesity-related metabolic disorders. We explored the association of genetic polymorphisms of: estrogen receptor alpha (ESR1-TA repeats); interleukin-6 (IL-6 G-174C); apolipoprotein E (APO epsilon2, epsilon3, epsilon4); lipoprotein lipase Pvu II (LPL P+/-), with clinical variables: gender, age, body mass index (BMI), diet type and biological variables: triglycerides, cholesterol, HDL-C, LDL-C, CRP, homocysteine, urate, and glucose in 105 healthy young subjects (20-35 yrs) of Croatian origin. ----- Methods Genotyping of IL-6, LPL was performed by PCR-RFLP, of APOE by real-time PCR, and of ESR1 by PCR and capillary electrophoresis. Association analyses were performed of alleles and genotypes with biological variables. ----- Conclusion ESR-1, LPL, and APO E genetic polymorphic variants could represent predictive genetic risk markers for obesity-related metabolic disorders in young healthy subjects. Mediterranean type of diet is also an important protective factor against abdominal obesity
INTEGRATION OF COMPLEMENTARY BIOMARKERS IN PATIENTS WITH FIRST EPISODE PSYCHOSIS: RESEARCH PROTOCOL OF A PROSPECTIVE FOLLOW UP STUDY
In this project, we recruited a sample of 150 patients with first episode of psychosis with schizophrenia features (FEP) and 100 healthy controls. We assessed the differences between these two groups, as well as the changes between the acute phase of illness and subsequent remission among patients over 18-month longitudinal follow-up. The assessments were divided into four work packages (WP): WP1- psychopathological status, neurocognitive functioning and emotional recognition; WP2- stress response measured by saliva cortisol during a stress paradigm; cerebral blood perfusion in the resting state (with single photon emission computed tomography (SPECT) and during activation paradigm (with Transcranial Ultrasonography Doppler (TCD); WP3-post mortem analysis in histologically prepared human cortical tissue of post mortem samples of subjects with schizophrenia in the region that synaptic alteration was suggested by WP1 and WP2; WP4- pharmacogenetic analysis (single gene polymorphisms and genome wide association study (GWAS). We expect that the analysis of these data will identify a set of markers that differentiate healthy controls from patients with FEP, and serve as an additional diagnostic tool in the first episode of psychosis, and prediction tool which can be then used to help tailoring individualized treatment options. In this paper, we describe the project protocol including aims and methods and provide a brief description of planned post mortem studies and pharmacogenetic analysis
Economic evaluation of pharmacogenomic-guided warfarin treatment for elderly Croatian atrial fibrillation patients with ischemic stroke
Background & methods: Economic evaluation in genomic medicine is an
emerging discipline to assess the cost-effectiveness of genome-guided
treatment. Here, we developed a pharmaco-economic model to assess
whether pharmacogenomic (PGx)-guided warfarin treatment of elderly
ischemic stroke patients with atrial fibrillation in Croatia is cost
effective compared with non-PGx therapy. The time horizon of the model
was set at 1 year. Results: Our primary analysis indicates that 97.07%
(95% CI: 94.08-99.34%) of patients belonging to the PGx-guided group
have not had any major complications, compared with the control group
(89.12%; 95% CI: 84.00-93.87%, p < 0.05). The total cost per patient
was estimated at Euro538.7 (95% CI: Euro526.3-551.2) for the PGx-guided
group versus Euro219.7 (95% CI: Euro137.9-304.2) for the control group.
In terms of quality-adjusted life-years (QALYs) gained, total QALYs was
estimated at 0.954 (95% CI: 0.943-0.964) and 0.944 (95% CI:
0.931-0.956) for the PGx-guided and the control groups, respectively.
The true difference in QALYs was estimated at 0.01 (95% CI:
0.005-0.015) in favor of the PGx-guided group. The incremental
cost-effectiveness ratio of the PGx-guided versus the control groups was
estimated at Euro31,225/QALY. Conclusion: Overall, our data indicate
that PGx-guided warfarin treatment may represent a cost-effective
therapy option for the management of elderly patients with atrial
fibrillation who developed ischemic stroke in Croatia. Original
submitted 4 June 2014; Revision submitted 12 November 201