Values of maximal relaxation (R_max,%) and pD₂ to acetylcholine (ACh), to sodium nitroprusside (SNP) and contraction forces induced by phenylephrine (10^-6M) in aortic rings in control, ischemic storage, ischemic storage and in vitro reperfusion, ischemic storage and in vivo 2h reperfusion, ischemic storage and in vivo 24h reperfusion, ischemic storage and in vivo 7d reperfusion group.

<p>Values represent mean ± SEM</p><p>^<i>a</i> P<.05 versus control</p><p>^<i>b</i> P<.05 versus ischemic storage</p><p>^<i>c</i> P<.05 versus ischemic storage + in vitro reperfusion</p><p>^<i>d</i> P<.05 versus ischemic storage + in vivo 2h reperfusion</p><p>^<i>e</i> P<.05 versus ischemic storage + in vivo 24h reperfusion</p><p>^<i>f</i> P<.05 versus ischemic storage + in vivo 7d reperfusion.</p><p>Values of maximal relaxation (R_max,%) and pD₂ to acetylcholine (ACh), to sodium nitroprusside (SNP) and contraction forces induced by phenylephrine (10^-6M) in aortic rings in control, ischemic storage, ischemic storage and in vitro reperfusion, ischemic storage and in vivo 2h reperfusion, ischemic storage and in vivo 24h reperfusion, ischemic storage and in vivo 7d reperfusion group.</p

DNA strand breaks and CD-31 positive cells in aortic rings.

<p>(A) endothelium-covered area (in percentage of the lumen area) (B) scoring of TUNEL staining (in percentage of total cell number) (Magnification x200, bar = 50μm) Values represent mean ± SEM; *P<.05 versus control; #P<.05 versus ischemic storage; §P<.05 versus ischemic storage and in vitro reperfusion; $P<.05 versus ischemic storage and in vivo 2h reperfusion.</p

Protein expressions of (A) cleaved bax, (B) bcl-2 (C), caspase-3 and (D) eNOS in aortic rings.

<p>Values represent median ± SEM; *P<.05 versus control; #P<.05 versus ischemic storage; ‡P<.05 versus ischemic storage and in vitro reperfusion.</p

Groups and treatments.

<p>Groups and treatments.</p

Effect of acute ethanol exposure on cardiac function.

<p>Assessment of (A) load-dependent (dP/dt_max) and (B) load-independent (dP/dt_max-EDV) contractility parameters revealed a significant decline only at 1 h in response to acute ethanol when compared with the control group. Whereas (C) maximal slope of the diastolic pressure decrement (dP/dt_min) 1 h and 6 h after ethanol administration was significantly decreased, (D) end-diastolic pressure-volume relationship (EDPVR) was significantly increased in rats treated with ethanol after 1 h, 6 h and 24 h. Eth indicates ethanol. *p<0.05 versus control.</p

The sequence for the forward (F) and reverse (R) primers (from 5′ to 3′) and Universal Probe Library (UPL) probes.

<p>Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), glutathione peroxidase (GPx)-4, L-Type calcium-channel (α1c-subunit; Ca_v1.2), sodium-potassium Adenosine Triphosphatase (Na⁺-K⁺-ATPase), sarco(endo)plasmic Ca²⁺-ATPas (SERCA)-2, superoxide dismutase (SOD)-1, matrix metalloproteinase (MMP)-9, tumor necrosis factor (TNF)-α, inducible nitric oxide synthase (NOS).</p

Effect of acute ethanol exposure on myocardial protein expression 24 h after transplantation.

<p>Twenty-four hours after transplantation, densitometric analysis of bands for (A) superoxide dismutase (SOD)-1 showed a significant increase at 6 h and 24 h following ethanol administration, at 6 h for (C) cytochrome-c oxidase (cyto-c oxi), and at 24 h for (D) matrix metalloproteinase (MMP)-9 compared with the control group. Moreover, 24 h after transplantation, there was a significant increase in protein expression for (B) glutathione peroxydase (GPx)-4 in the group receiving ethanol 24 h prior to explantation when compared with the control- and 6 h ethanol+24 I/R groups. I/R indicates ischemia/reperfusion, Eth ethanol, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). *p<0.05 versus 24 h I/R group; #p<0.05 versus 6 h Eth+ 24 h I/R group.</p

Cardiac hemodynamic parameters.

<p>SBP: systolic blood pressure; DBP: diastolic blood pressure; MAP: mean arterial pressure; LVESP: left-ventricular end-systolic pressure; LVEDP: left-ventricular end-diastolic pressure.</p>*<p>p<0.05 versus control;</p>#<p>p<0.05 versus ethanol 1 h.</p