Independent variables for loss to follow-up in HIV care.

<p>^a Odds ratio</p><p>^b Mantel-Haenszel method for binominal variables and conditional logistic regression for ordinal variables</p><p>^c Confidence interval</p><p>^d Stepwise, multivariate-adjusted conditional logistic regression</p

Analysis of effects of genotype on parameters of HIV disease progression in the HIV-1-infected cohort.

<p>(A) Changes in CD4⁺ T cell counts (cells/μl/day) (n = 202). (B) Changes in HIV-1 RNA levels (log₁₀ copies/ml/day) in plasma (n = 202). The box plots show data between the 25th and 75th percentiles with central horizontal lines representing the median, and with whiskers showing the 10th and 90th percentiles. The open circles represent outliers with data >1.5-fold of the interquartile range. All the p values were determined using the Kruskal-Wallis test.</p

APOBEC3 mRNA expression levels depending on <i>APOBEC3B</i> genotype.

<p>(A) Comparison of mRNA expression levels of APOBEC3 in CD4⁺ cells isolated from intact (I/I), hemizygous (I/D) and deletion (D/D) individuals of healthy donors. The relative mRNA expression levels of APOBEC3A (I/I, n = 8; I/D, n = 4; D/D, n = 4), APOBEC3B (I/I, n = 11; I/D, n = 7; D/D, n = 5), APOBEC3C (I/I, n = 12; I/D, n = 7; D/D, n = 5), APOBEC3DE (I/I, n = 11; I/D, n = 9; D/D, n = 5), APOBEC3F (I/I, n = 12; I/D, n = 7; D/D, n = 5), APOBEC3G (I/I, n = 12; I/D, n = 7; D/D, n = 5), and APOBEC3H (I/I, n = 6; I/D, n = 7; D/D, n = 4) were determined using quantitative RT-PCR and were normalized to GAPDH. The red (I/I) or gray (D/D) dots represent the expression levels of donors whose PBMCs were used for the <i>in vitro</i> kinetics of HIV-1 replication and infectivity in Figure. 3. The p values were calculated using Welch’s <i>t</i>-test. The error bar represents the standard error of the mean (SEM). (B) APOBEC3A (A3A) and APOBEC3G (A3G) mRNA expression levels under basal conditions (Ctrl) and after stimulation with 100 U/ml (+IFN-α) of interferon (IFN)-α in CD14⁺ MDMs isolated from healthy control subjects. The black and white bars indicate D/D (n = 3) and I/I (n = 4) individuals, respectively. The <i>p</i> values were calculated with the Mann-Whitney <i>U</i>-test. The error bars represent the standard deviation. <i>n.d.</i>, not detected. Ct, cycle threshold. <i>n.s.</i>, not significant (<i>p</i> = 0.4 for both cases).</p

Demographics of the cohorts.

a<p>IQR denotes interquartile range.</p>b<p>NA, Not applicable.</p

<i>APOBEC3B</i> genotype frequency and clinical parameters in HIV-1-positive patients.

a<p>Determined using the Fischer exact test.</p

<i>APOBEC3B</i> genotype frequency on the days from diagnosis to ART (n = 246).

<p>(Median days from diagnosis to ART = 88 days).</p>a<p>Determined using the Fischer exact test.</p><p>The diagnosis date of 2 patients(each patient’s genotype is I/I and I/D, respectively.) are unknown.</p