Diurnal changes in chloroplast positioning and photosynthetic traits of C₄ grass finger millet

Mesophyll (M) chloroplasts in finger millet are known to aggregate to the bundle sheath side when leaves are constantly irradiated with extremely high-intensity light. This aggregative movement of M chloroplasts is also observed in natural environment, but whether a natural light regime is effective in inducing the response remains unclear. Abscisic acid is reported to trigger not only the aggregative movement but also stomatal closure, but photosynthetic responses accompanying the aggregative movement also remain unknown.We investigated changes in chloroplast positioning and photosynthetic traits under diurnal patterns of light, mimicking the natural light environment. M chloroplasts showed the aggregative movement with increasing light intensity whether it frequently fluctuated or not, and kept their aggregative positions in the midday. With decreasing light intensity, M chloroplasts returned to the random position in the evening. These results suggest that M chloroplasts often rearrange their intracellular positions during the daytime and that the chloroplast aggregative movement can be induced by a natural regime of light. The chloroplast aggregative movement was observed with increasing stomatal conductance, suggesting that stomatal closure is not crucial to trigger the chloroplast response

Light stress-induced chloroplast movement and midday depression of photosynthesis in sorghum leaves

Plants are exposed to high light intensity, high leaf temperatures and high air-to-leaf water vapor pressure deficit (ALVPD) during the day. These environmental stresses cause stomatal closure and photoinhibitory damage, leading to midday depression of photosynthesis. Chloroplast positioning is essential for the efficient operation of photosynthesis. However, chloroplast behavior before, during, and even after the midday depression of photosynthesis remains unknown. We investigated changes in the intracellular positioning of chloroplasts and photosynthetic traits under a diurnal pattern of light. Sorghum leaves were exposed to a 12-h regime of light mimicking the natural light environment, with constant leaf temperature and ALVPD. Net photosynthetic rate (Pn) showed a diurnal pattern, and midday depression in Pn was observed at 3.8 h of irradiation. Depression in Pn was attributed to stomatal limitation because the decrease in Pn was in accordance with the decrease in stomatal conductance. The maximum efficiency of photosystem II decreased with the increase in light intensity and remained low after 12 h of irradiation. Bundle sheath chloroplasts swelled after 8 h of irradiation, representing the accumulation of starch. Conversely, mesophyll chloroplasts exhibited avoidance response after 4 h of irradiation, and the avoidance position was maintained during the remainder of the daytime. These data suggest that chloroplasts are subject to light stress during and after the midday depression of photosynthesis. The intensity of natural light is excessive for most of the day and this light stress induces chloroplast avoidance response and depression of photosynthesis

Energy Production from Fish Waste in Large-scale Anaerobic Digestion

Poster Presentatio

Characteristics of Dredged Sludges by Desiccation

To examine the possible reuse of dredged sludge in view of construction material, physico-chemical properties and geotechnical characteristics of desiccated sludge are investigated through laboratory experiments. The consistency test, the pH test, the XRF test and the X-ray diffraction analysis are carried out for physico-chemical proteries, and the conolidation test and the permeability test are made for geotechnical characteristics. It is concluded that the history of desiccation changes the consistency of sludge and affects its permeability and consolidation bahavior

The first choristoderan record from the Upper Cretaceous of Asia, Tamagawa Formation, Kuji Group, Japan

Choristoderes are freshwater diapsid reptiles that are distributed through Laurasia in Jurassic–Miocene deposits. The group shows great diversity in the Early Cretaceous of Asia, with all recognized morphotypes recorded from that region. However, there is then a substantial gap in the Asian record until choristoderes are reported from the Paleocene of Kazakhstan. This gap has raised questions as to whether the group became extinct in Asia during the Late Cretaceous, with subsequent reinvasion from either North America or Europe. Here we report the discovery of vertebrae attributable to Choristodera indet. from the lower Upper Cretaceous (Turonian) of the Tamagawa Formation, Kuji City, Iwate Prefecture, Japan. This is the first record of Choristodera from the Upper Cretaceous of Asia, and may imply that the group persisted in this region from the Jurassic to the Paleocene. The challenge for the future will be to recover a more complete record of Choristodera in the Upper Cretaceous of Asia

再利用性と軌道上再構成能力に優れた衛星ソフトウエア・アーキテクチャに関する研究

学位の種別: 課程博士審査委員会委員 : (主査)東京大学教授 中須賀 真一, 東京大学教授 堀 浩一, 東京大学准教授 矢入 健久, 東京大学准教授 船瀬 龍, 東京理科大学教授 木村 真一University of Tokyo(東京大学

MIG-seq is an effective method for high-throughput genotyping in wheat (Triticum spp.)

MIG-seq (Multiplexed inter-simple sequence repeats genotyping by sequencing) has been developed as a low cost genotyping technology, although the number of polymorphisms obtained is assumed to be minimal, resulting in the low application of this technique to analyses of agricultural plants. We applied MIG-seq to 12 plant species that include various crops and investigated the relationship between genome size and the number of bases that can be stably sequenced. The genome size and the number of loci, which can be sequenced by MIG-seq, are positively correlated. This is due to the linkage between genome size and the number of simple sequence repeats (SSRs) through the genome. The applicability of MIG-seq to population structure analysis, linkage mapping, and quantitative trait loci (QTL) analysis in wheat, which has a relatively large genome, was further evaluated. The results of population structure analysis for tetraploid wheat showed the differences among collection sites and subspecies, which agreed with previous findings. Additionally, in wheat biparental mapping populations, over 3, 000 SNPs/indels with low deficiency were detected using MIG-seq, and the QTL analysis was able to detect recognized flowering-related genes. These results revealed the effectiveness of MIG-seq for genomic analysis of agricultural plants with large genomes, including wheat