sj-tiff-3-cll-10.1177_09636897241228026 – Supplemental material for Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation

Supplemental material, sj-tiff-3-cll-10.1177_09636897241228026 for Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation by Masayuki Kitano, Yasuyuki Hayashi, Hiroe Ohnishi, Hideaki Okuyama, Masayoshi Yoshimatsu, Keisuke Mizuno, Fumihiko Kuwata, Takeshi Tada, Yo Kishimoto, Satoshi Morita and Koichi Omori in Cell Transplantation</p

sj-docx-1-cll-10.1177_09636897241228026 – Supplemental material for Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation

Supplemental material, sj-docx-1-cll-10.1177_09636897241228026 for Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation by Masayuki Kitano, Yasuyuki Hayashi, Hiroe Ohnishi, Hideaki Okuyama, Masayoshi Yoshimatsu, Keisuke Mizuno, Fumihiko Kuwata, Takeshi Tada, Yo Kishimoto, Satoshi Morita and Koichi Omori in Cell Transplantation</p

sj-tiff-2-cll-10.1177_09636897241228026 – Supplemental material for Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation

Supplemental material, sj-tiff-2-cll-10.1177_09636897241228026 for Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation by Masayuki Kitano, Yasuyuki Hayashi, Hiroe Ohnishi, Hideaki Okuyama, Masayoshi Yoshimatsu, Keisuke Mizuno, Fumihiko Kuwata, Takeshi Tada, Yo Kishimoto, Satoshi Morita and Koichi Omori in Cell Transplantation</p

Representative images of immunohistochemistry for FUT8 and p53 expression in colorectal cancer.

<p>(A) FUT8 protein expression in colon carcinoma [T] and adjacent colon mucosa [N]. (B) FUT8 was not expressed by non-neoplastic colon mucosal cells. (C) FUT8 staining was typically found in cytoplasm of tumor cells. (D) Occasionally, concomitant cytoplasmic and membranous staining of FUT8 in tumor cells can be found. (E) p53-positive tumor showing strong nuclear staining in cancer cells. (F) p53-negative tumor showing no nuclear staining. Magnification: (A,E,F) x100; (B,C,D) x400.</p

Prognostic role of FUT8 expression in relation to p53 status in stage II and III colorectal cancer

<div><p>The expression of fucosyltransferase 8, an enzyme responsible for core fucosylation encoded by FUT8, influences tumor biology and correlates with patient prognosis in several solid cancers. We hypothesized that p53 alteration modifies prognostic associations of FUT8 expression in colorectal cancer (CRC), since FUT8 has recently been identified as a direct transcriptional target of wild-type p53. Utilizing multiple datasets of microarray and RNA sequence of CRC, FUT8 mRNA was found to be highly expressed in wild-type p53 tumors (n = 382) compared to those of mutant p53 (n = 437). Prognostic values of FUT8 expression in conjunction with the p53 status for disease-free survival (DFS) were analyzed using two independent cohorts of stage II and III CRC after curative surgery, including the immunohistochemistry (IHC) cohort (n = 123) and the microarray cohort (n = 357). In both cohorts, neither FUT8 expression nor the p53 status was associated with DFS. Strikingly, positive expression of FUT8 protein was significantly associated with better DFS only in tumors with negative p53, while it had no prognostic impact in tumors with positive p53 in the IHC cohort. Although not statistically significant, a similar prognostic trend was observed in the microarray cohort when patients were stratified by the p53 status. Our results suggest that the prognostic values of FUT8 expression on DFS may be modified by the p53 status, and the expression of FUT8 protein can be a prognostic biomarker for patients with stage II and III CRC.</p></div

Univariate and multivariate Cox regression analysis for disease-free survival in patients with stage II and III colorectal cancer according to FUT8 expression and the p53 status.

<p>Univariate and multivariate Cox regression analysis for disease-free survival in patients with stage II and III colorectal cancer according to FUT8 expression and the p53 status.</p

Clinicopathological characteristics of colorectal cancer patients according to FUT8 expression.

<p>Clinicopathological characteristics of colorectal cancer patients according to FUT8 expression.</p

Univariate and multivariate Cox regression analysis for disease-free survival in patients with stage II and III colorectal cancer.

<p>Univariate and multivariate Cox regression analysis for disease-free survival in patients with stage II and III colorectal cancer.</p

The expression of FUT8 mRNA in multiple cohorts of colorectal cancer.

<p>(A) FUT8 mRNA expression was significantly upregulated in primary tumors compared to normal colon mucosa. (B) In five independent datasets of colorectal cancer, higher levels of FUT8 mRNA expression were consistently observed in tumors with wild-type p53 than those of mutant p53.</p

Kaplan-Meier event curves for clinical outcomes in patients with two-vessel or three-vessel disease including proximal LAD.

(TIF)</p