19 research outputs found
A glutathione s-transferase confers herbicide tolerance in rice
Plant glutathione S-transferases (GSTs) have been a focus of attention due to their role in herbicide detoxification. OsGSTL2
is a glutathione S-transferase, lambda class gene from rice (Oryza sativa L.). Transgenic rice plants over-expressing OsGSTL2 were
generated from rice calli by the use of an Agrobacterium transformation system, and were screened by a combination of hygromycin
resistance, PCR and Southern blot analysis. In the vegetative tissues of transgenic rice plants, the over-expression of OsGSTL2 not
only increased levels of OsGSTL2 transcripts, but also GST and GPX expression, while reduced superoxide. Transgenic rice plants
also showed higher tolerance to glyphosate and chlorsulfuron, which often contaminate agricultural fields. The findings demonstrate
the detoxification role of OsGSTL2 in the growth and development of rice plants. It should be possible to apply the present results to
crops for developing herbicide tolerance and for limiting herbicide contamination in the food chain