Cultural characteristics of Laetiporus sulphureus, producing an anti-thrombin substance

The clotting time for thrombin of the culture broth from Laetiporus sulphureus was more than 44 times that of the control (2% malt extract medium), whereas the culture broths of all other strains exhibited lower anti-coagulative activity. In experiments on the utilization of carbon sources, the mycelial growth of L. sulphureus was rapid with glucose as the sole carbon source. Casamino acid was the best source for the strain. Thiamine was required for the mycelial growth of L. sulphureus. The optimum temperature for efficient mycelial growth was recorded at 30℃. The initial pH 4 to 5 was the most favorable for L. sulphureus for growth