English prosodic marking of Information Structure by L1-Japanese second language learners

Ph.D. Thesis. University of Hawaiʻi at Mānoa 2018

Analysis and comparison of the junior and senior high school level English textbooks for Japan and Korea

Japan and Korea share similar motivations towards EFL education, acknowledging the necessity of English ability for each nation’s further development. Although both countries outline similar EFL philosophies in their course-of-study guidelines, there seem to be differences in the ways the guidelines are reflected in the textbooks. This paper compares and summarizes the organization, content, linguistic skills, and tasks of the Japanese and Korean English textbooks used in 3rd-year junior high and 1st-year high school. The continuity of the textbooks between the two grades was also analyzed. The framework of educational approach, the balance of four skills, the continuity of the level and the difficulty of the text were the major observed differences. Learning through the differences and similarities, considerations for future textbook writers are suggested

Effects of contrastive intonation and grammatical aspect on processing coreference in Mainstream American English

Coreference choices are influenced by multiple factors, including information structural categories such as topic and focus. These information structural categories can be indicated by intonation, yet few studies have investigated how intonation affects subsequent choices for coreference. Using a story continuation experiment with aurally presented stimuli, we show that the location of contrastive focus in Mainstream American English significantly affects the preferred referent for the subject of the next sentence in a short discourse

microRNA-345の過剰発現は、MUC1およびTJP2の発現を抑制することにより、膵管腺癌細胞株の浸潤能に影響を及ぼす

The majority of pancreatic carcinomas are pancreatic ductal adenocarcinomas (PDAC), and the presence of non-invasive pancreatic intraepithelial neoplasia or intraductal papillary mucinous neoplasm, as an associated lesion, is considered important. These microscopic hyperplastic or grossly papillomatous lesions exhibit varying degrees of morphological atypia and may develop into invasive carcinomas. In this study, we investigated whether mucin-1 (MUC1) is involved in the progression of pancreatic carcinoma and examined the mechanisms by which microRNAs regulate MUC1 expression in vitro. In PDAC cell lines, suppression of MUC1 expression reduced cell proliferation and invasion; PDAC cell lines transfected with an miR-345 precursor suppressed the expression of MUC1, and reduced cell proliferation and invasion. Tight junction protein 2 (TJP2), a putative target of miR-345, is regulated by MUC1. The suppression of TJP2 expression reduced cell proliferation by inducing apoptosis. These results suggest that MUC1 and TJP2, the putative target molecules of miR-345, are critical in maintaining the invasive potential of pancreatic carcinoma cells, and regulating their expression may prevent the progression of non-invasive pancreatic intraductal lesions to invasive carcinomas. This study provides new insights for the development of novel molecular targeted therapies for pancreatic carcinomas.博士（医学）・甲第866号・令和5年3月15

Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues

Adipose-derived stem cells (ASCs) are an attractive cell source for cell therapy. Despite the increasing number of clinical applications, the methodology for ASC isolation is not optimized for every individual. In this study, we developed an effective material to stabilize explant cultures from small-fragment adipose tissues. Methods: Polypropylene/polyethylene nonwoven sheets were coated with hydroxyapatite (HA) particles. Adipose fragments were then placed on these sheets, and their ability to trap tissue was monitored during explant culture. The yield and properties of the cells were compared to those of cells isolated by conventional collagenase digestion. Results: Hydroxyapatite-coated nonwovens immediately trapped adipose fragments when placed on the sheets. The adhesion was stable even in culture media, leading to cell migration and proliferation from the tissue along with the nonwoven fibers. A higher fiber density further enhanced cell growth. Although cells on nonwoven explants could not be fully collected with cell dissociation enzymes, the cell yield was significantly higher than that of conventional monolayer culture without impacting stem cell properties. Conclusions: Hydroxyapatite-coated nonwovens are useful for the effective primary explant culture of connective tissues without enzymatic cell dissociation