Representative histopathology of the optic nerves in EAE mice.

<p>(A) Optic nerves were stained with luxol fast blue (LFB) and hematoxylin and eosin (HE) (upper two panels) or NF200 and toluidine blue for the semithin transverse sections (lower two panels). The arrows point to the degenerating axons. Scale bar: 100 µm for the first and third panels, 75 µm for the second panels and 15 µm for the lower panels. (B) Quantitative analysis of cell infiltrates in the longitudinal section of the optic nerve. (C) Quantitative analysis of degenerating axons in the transverse section of the optic nerve. *<i>P</i><0.01.</p

The effect of Olig1 deficiency on the number of oligodendrocytes and their progenitor cells during EAE.

<p>(A) Representative immunostaining of CC1 and PDGFR-α in the white matter of the spinal cord. Scale bar: 40 µm and applies to all panels. (B) Quantitative analysis of mature oligodendrocytes and their progenitor cells. *<i>P</i><0.001. OLG, oligodendrocyte; OPC, oligodendrocyte progenitor cell.</p

The effect of Olig1 deficiency on T cell proliferation capability.

<p>(A) Proliferative responses of MOG-specific T cells isolated from WT and Olig1^−/− EAE mice (<i>n</i> = 4). (B) RT-PCR analysis of Olig1 expression in brain, spinal cord, lymph node, spleen and thymus of WT mice.</p

Quantification of histopathology of the spinal cords in EAE mice.

<p>Quantitative analysis of the extent of demyelination (A), GFAP-positive (B) and iba1-positive (C) cells in the spinal cord. The areas of demyelinated regions in the white matter were measured by ImageJ 1.43u and expressed as a percentage of the whole area of the white matter. GFAP- and iba1-positive cells were counted per unit area (0.143 mm²) in the middle region of the ventral horn. ***<i>P</i><0.001; **<i>P</i><0.01; *<i>P</i><0.05.</p

Histopathology of the spinal cords in EAE mice.

<p>Representative histology of the spinal cords of WT (A) and Olig1^−/− mice (B) in d10, d20 and d60. Lumbar spinal cords were stained with luxol fast blue (LFB) and hematoxylin and eosin (HE) (upper panels) and either an anti-GFAP (middle panels) or anti-iba1 antibody (lower panels). Scale bar: 200 µm and applies to all panels.</p

Delayed EAE disease onset in Olig1^−/− mice.

<p>(A) EAE disease incidence in wild-type (WT; <i>n</i> = 17) and Olig1^−/− (<i>n</i> = 20) mice. (B) Clinical evaluation of WT and Olig1^−/− EAE mice during a period of 60 days after MOG immunization. (C) Clinical scores of WT and Olig1^−/− EAE mice in different stages.</p