QUALITY CONTROL OF [18F]FDG INJECTION: BACTERIAL ENDOTOXIN TEST, ALUMINUM IONS TEST AND HPLC ANALYSIS FOR FDG AND ClDG

ドイツのアーヘンで８月３０日から９月３日まで開催された「Sixth InternationalConference on Nuclear and Radiochemistry」に出席した。会議では、演題「QUALITY CONTROL OF [18F]FDG INJECTION: BACTERIAL ENDOTOXIN TEST, ALUMINUMIONS TEST AND HPLC ANALYSIS FOR FDG AND CLDG」を発表した。Sixth International Conference on Nuclear and radiochemistr

Sensitive measurement of positron emitters eluted from HPLC

For sensitive analysis of the radioactive-metabolite in human PET, a radio-HPLC system coupled to a newly designed positron detector was constructed. The detector had the advantages of low noise level(1.7+/-1.0cpm)and high sensitivity(32+/-1%)due to coincidence counting and large BGO crystals. Furthermore, the detector was easy to move,since a pair of the BGO housings coupled to photomultipliers was effectively arranged in parallel and a HPLC cell with different volume could be inserted between the BGO housing. This radio-HPLC system was useful for analyzing samples with low radioactivity. It was applied to the measurement of [11C]FLB457in plasma,having high affinity and high selectivity with dopamine D2 receptors. Extremely low radioactivity of [11C]FLB457(2500dpm)could be analzed by using the radio-HPLC system. The performance of this detector was compared with those of commercially available systems that had been used as sensitive detectors for HPLC. (C)2001 Elsevier Science Ltd. All rights reserved

Quality control of [18F]FDG injection: Bacterial endotoxins test, aluminum ions test and HPLC analysis for FDG and ClDG

ドイツのアーヘンで８月３０日から９月３日まで開催された「Sixth InternationalConference on Nuclear and Radiochemistry」に出席した。会議では、演題「QUALITY CONTROL OF [18F]FDG INJECTION: BACTERIAL ENDOTOXIN TEST, ALUMINUMIONS TEST AND HPLC ANALYSIS FOR FDG AND CLDG」を発表した。Sixth International Conference on Nuclear and radiochemistr

Factors affecting quality control of [18F]FDG injection: bacterial endotoxins test, aluminum ions test and HPLC analysis for FDG and ClDG

ドイツのアーヘンで８月３０日から９月３日まで開催された「Sixth InternationalConference on Nuclear and Radiochemistry」に出席した。会議では、演題「QUALITY CONTROL OF [18F]FDG INJECTION: BACTERIAL ENDOTOXIN TEST, ALUMINUMIONS TEST AND HPLC ANALYSIS FOR FDG AND CLDG」を発表した。Sixth International Conference on Nuclear and radiochemistr

[11C]DAA1106: Radiosynthesis and in vivo binding to peripheral benzodiazepine receptors in mouse brain

DAA1106 (N-(2,5-Dimethoxybenzyl)-N-(5-fluoro-2-phenoxyphenyl)acetamide), is a potent and selective ligand for peripheral benzodiazepine receptors (PBR) in mitochondrial fractions of rat (Ki=0.043 nM) and monkey (Ki=0.188 nM) brains. This compound was labeled by [11C]methylation of a corresponding desmethyl precursor (DAA1123) with [11C]CH3I in the presence of NaH, with a 72+/-16% (corrected for decay) incorporation yield of radioactivity. After HPLC purification, [11C]DAA1106 was obtained with >98% radiochemical purity and specific activity of 90-156 GBq/mumol at the end of synthesis. After iv injection of [11C]DAA1106 into mice, high accumulations of radioactivity were found in the olfactory bulb and cerebellum, the high PBR density regions in the brain. Coinjection of [11C]DAA1106 with unlabeled DAA1106 and PBR-selective PK11195 displayed a significant reduction of radioactivity, suggesting a high specific binding of [11C]DAA1106 to PBR. Although this tracer was rapidly metabolized in the plasma, only [11C]DAA1106 was detected in the brain tissues, suggesting the specific binding in the brain due to the tracer itself. These findings revealed that [11C]DAA1106 is a potential and selective positron emitting radioligand for PBR

[11C]DAA1106: Radiosynthesis and in vivo binding to peripheral benzodiazepine receptors in mouse brain

DAA1106 (N-(2,5-Dimethoxybenzyl)-N-(5-fluoro-2-phenoxyphenyl)acetamide), is a potent and selective ligand for peripheral benzodiazepine receptors (PBR) in mitochondrial fractions of rat (Ki=0.043 nM) and monkey (Ki=0.188 nM) brains. This compound was labeled by [11C]methylation of a corresponding desmethyl precursor (DAA1123) with [11C]CH3I in the presence of NaH, with a 72+/-16% (corrected for decay) incorporation yield of radioactivity. After HPLC purification, [11C]DAA1106 was obtained with >98% radiochemical purity and specific activity of 90-156 GBq/mumol at the end of synthesis. After iv injection of [11C]DAA1106 into mice, high accumulations of radioactivity were found in the olfactory bulb and cerebellum, the high PBR density regions in the brain. Coinjection of [11C]DAA1106 with unlabeled DAA1106 and PBR-selective PK11195 displayed a significant reduction of radioactivity, suggesting a high specific binding of [11C]DAA1106 to PBR. Although this tracer was rapidly metabolized in the plasma, only [11C]DAA1106 was detected in the brain tissues, suggesting the specific binding in the brain due to the tracer itself. These findings revealed that [11C]DAA1106 is a potential and selective positron emitting radioligand for PBR