化学発光検出高速液体クロマトグラフィを用いたヒト尿及び毛髪からの覚せい剤分析に関する研究

取得学位：博士(薬学)，学位授与番号：博乙第175号，学位授与年月日：平成10年9月30日,学位授与年：199

High-Sensitivity Analysis of Cyanide by Capillary Electrophoresis with Fluorescence Detection

金沢大学大学院自然科学研究科物質情報解析金沢大学工学部A capillary electrophoretic method for a high-sensitivity analysis of cyanide has been developed. Cyanide was derivatized with 2,3-naphthalenedialdehyde and taurine to give a fluorescent product of 1-cyanobenz[f]isoindole. This compound was detected with high sensitivity by fluorescence detection. The detection limit was 0.1 ng/mL, and the calibration curve was linear over the range 0.1 - 200 ng/mL. The precision of the migration time of within-run assays (n = 6) of 1 ng/mL cyanide standard solution was 0.14%. The precision of the peak area for the same runs was 1.0%. This method was applicable to blood analysis. Detection of the cyanide derivative by UV was also examined

Chiral Capillary Electrophoresis of Amphetamine-Type Stimulants

Amphetamine-type stimulants (ATS), having one or more asymmetric carbons, are important targets in forensic science field. For chiral analysis, capillary electrophoresis (CE) has advantages over other chromatographic methods, especially, in fastness, resolution, cost performance and simplicity. This review summarizes chiral separation techniques of ATS using CE with UV detection or CE/mass spectrometry mainly by the present authors\u27 research group