Effects of heat treatment on human hair keratin film

5ArticleJOURNAL OF JAPANESE COSMETIC SCIENCE SOCIETY. 37(3):165-170 (2013)journal articl

Redescription of Sarsia japonica (Nagao) (Hydrozoa : Corynidae) from Hokkaido, Japan

Sarsia japonica (Nagao) is redescribed and illustrated, based on I female and 6 male colonies collected from Muroran (a new locality) and Akkeshi (the type locality), Hokkaido, Japan. Previously undescribed character states of both the hydroid and the laboratory-reared medusa are noted and the substrata occupied by this species and its geographical distribution are summarized. Sarsia japonica is judged to be a distinct species, and the characteristics that distinguish its hydroid and medusa from those of its closest congener, S. producta (Wright), are pointed out

Marine Tubificidae (Oligochaeta, Annelida) from Shirahama, Western Japan, With a Description of a New Species

Three species of manne Tubificidae, including one new to science are described from Shirahama, Wakayama Prefecture, western Japan. Heterodrilus mediopapillosus sp. n. is characterized by having median papilla in ventral of X, pear-shaped pseudopenes, long penial setae, and presence of spermathecae. Aktedrilus locyi and Limnodriloides tenuiductus are reported from Japan for the first time

Detection of revertant mosaicism in epidermolysis bullosa through Cas9-targeted long-read sequencing

Revertant mosaicism (RM) is a phenomenon in which inherited mutations are spontaneously corrected in somatic cells. RM occurs in some congenital skin diseases, but genetic validation of RM in clinically revertant skin has been challenging, especially when homologous recombination (HR) is responsible for RM. Here, we introduce nanopore Cas9-targeted sequencing (nCATS) for identifying HR in clinically revertant skin. We took advantage of compound heterozygous COL7A1 mutations in a patient with recessive dystrophic epidermolysis bullosa who showed revertant skin spots. Cas9-mediated enrichment of genomic DNA (gDNA) covering the two mutation sites (>8 kb) in COL7A1 and subsequent MinION sequencing successfully detected intragenic crossover in the epidermis of the clinically revertant skin. This method enables the discernment of haplotypes of up to a few tens of kilobases of gDNA. Moreover, it is devoid of polymerase chain reaction amplification, which can technically induce recombination. We, therefore, propose that nCATS is a powerful tool for understanding complicated gene modifications, including RM