33 research outputs found
Laser Capture Microdissection in Dentistry
Laser capture microdissection (LCM) allows for the microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. According to the recent development of the LCM technologies and methodologies, the LCM has been used in various kinds of tissue specimens in dental research. For example, the real-time polymerase-chain reaction (PCR) can be performed from the formaldehyde-fixed, paraffin-embedded, and immunostained sections. Thus, the advance of immuno-LCM method allows us to improve the validity of molecular biological analysis and to get more accurate diagnosis in pathological field in contrast to conventional LCM. This paper is focused on the presentation and discussion of the existing literature that covers the fields of RNA analysis following LCM in dentistry
Reparative Dentinogenesis Induced by Mineral Trioxide Aggregate: A Review from the Biological and Physicochemical Points of View
This paper aims to
review the biological and physicochemical
properties of mineral trioxide aggregate (MTA)
with respect to its ability to induce reparative
dentinogenesis, which involves complex cellular
and molecular events leading to hard-tissue
repair by newly differentiated odontoblast-like
cells. Compared with that of calcium
hydroxide-based materials, MTA is more efficient
at inducing reparative dentinogenesis in vivo.
The available literature suggests that the
action of MTA is attributable to the natural
wound healing process of exposed pulps, although
MTA can stimulate hard-tissue-forming cells to
induce matrix formation and mineralization in
vitro. Physicochemical analyses have revealed
that MTA not only acts as a “calcium
hydroxide-releasing” material, but also
interacts with phosphate-containing fluids to
form apatite precipitates. MTA also shows better
sealing ability and structural stability, but
less potent antimicrobial activity compared with
that of calcium hydroxide. The clinical outcome
of direct pulp capping and pulpotomy with MTA
appears quite favorable, although the number of
controled prospective studies is still limited.
Attempts are being conducted to improve the
properties of MTA by the addition of setting
accelerators and the development of new calcium
silicate-based materials