2 research outputs found
Development of an Activatable Fluorescent Probe for Prostate Cancer Imaging
Technology
to visualize small prostate cancers is urgently needed
because of the difficulty of discriminating prostate cancer from normal
tissue with the naked eye, and a fluorescence imaging method would
be advantageous. Here, we describe the design and synthesis of a fluorogenic
probe (Ac–KQLR–HMRG) that is activated by hepsin and
matriptase (proteases over-expressed in prostate cancer). Ac–KQLR–HMRG
exhibited significant turn-on fluorogenicity in the presence of hepsin
(180-fold) and matriptase (80-fold) and allowed specific fluorescence
imaging of various prostate cancer cell line in vitro. In addition,
the probe enabled rapid imaging (within 1–10 min) of small
prostate cancer nodules in mouse models of disseminated peritoneal
tumor and orthotopic tumor