Therapeutic effect of anti-TNF mAb, CTLA-4Ig, and anti-IL-6 mAb in GPI-induced arthritis

Glucose-6-phosphate isomerase (GPI)-immunized mice were treated with anti-tumor necrosis factor (TNF)-α mAb; anti-IFN-γ mAb or anti-IL-12 mAb; cytotoxic T-lymphocyte antigen 4 immunoglobulin (CTLA-4Ig; on days 8 and 12); CTLA-4Ig (on days 12 and 16); and or anti-IL-6 mAb just after the onset of arthritis (on day 8, on days 8 and 12, or days 12 and 16; arrow). The mean clinical index (± standard error) was examined throughout the study. *< 0.05, **< 0.01, by Mann-Whitney's U test. n = 6 mice in each group. Hematoxylin and eosin staining at day 21 (×40) is also shown: anti-TNF-α mAb, control antibody, and CTLA-4Ig (on days 8 and 12).<p><b>Copyright information:</b></p><p>Taken from "Therapeutic effects of antibodies to tumor necrosis factor-α, interleukin-6 and cytotoxic T-lymphocyte antigen 4 immunoglobulin in mice with glucose-6-phosphate isomerase induced arthritis"</p><p>http://arthritis-research.com/content/10/3/R66</p><p>Arthritis Research & Therapy 2008;10(3):R66-R66.</p><p>Published online 5 Jun 2008</p><p>PMCID:PMC2483457.</p><p></p

Concentration of inflammatory cytokines in serum of mice with GPI-induced arthritis

After immunization with glucose-6-phosphate isomerase (GPI), serum samples were collected from GPI-induced DBA/1 mice at the indicated time points (days 0, 7, 14, and 28). Serum concentrations of tumor necrosis factor (TNF)-α (solid circle), IL-6 (open square), IL-1β (open triangle), or IFN-γ (open diamond) were determined by enzyme-linked immunosorbent assay. Data are expressed as mean ± standard error. = 3 mice in each group. *< 0.05, by Mann-Whitney's U-test.<p><b>Copyright information:</b></p><p>Taken from "Therapeutic effects of antibodies to tumor necrosis factor-α, interleukin-6 and cytotoxic T-lymphocyte antigen 4 immunoglobulin in mice with glucose-6-phosphate isomerase induced arthritis"</p><p>http://arthritis-research.com/content/10/3/R66</p><p>Arthritis Research & Therapy 2008;10(3):R66-R66.</p><p>Published online 5 Jun 2008</p><p>PMCID:PMC2483457.</p><p></p