22 research outputs found
Nano-honeycomb electrode based QCM sensor and its application for PPI detection
An anodic aluminon oxide (AAO)-based nano honeycomb electrode was coated on a quartz crystal for use as a sensing device in a quartz crystal microbalance (QCM) to monitor the protein-protein interactions (PPIs). As examples, we detected PPIs of anti-Bcl-2 and Bcl-2, and Bcl-2 and Bax in real time. A sensor with a flat Au electrode showed a tiny frequency shift upon sample injection. However, our fabricated device could detect 10 μg/mL of Bcl-2 and Bax in sequence.2016年度関西大学研究拠点形成支援経
SYNTHESIS OF SUBSTITUTED t-BUTYL 3-ALKYLOXINDOLE-3-CARBOXYLATES FROM DI-t-BUTYL (2-NITROPHENYL)MALONATES
Using a novel tandem reduction-cyclization, we synthesized t-butyl 3-alkyloxindole-3-carboxylates from the di-t-butyl 2-alkyl-2-(2-nitrophenyl)malonate. Introduction of an α-substituent to the di-t-butyl 2-(2-nitrophenyl)-malonates and addition of acid promoted reactivity. This methodology was successfully applied to gram-scale-synthesis of the t-butyl 3-methyloxindole-3-carboxylate 1 and 3-hydroxymethyl-3-methyloxindole 2 without silica gel column chromatography.Part of this work was supported by the Kansai University Subsidy for Supporting Young Scholars, 2015 “Development of macrocyclization reaction via a photoaffinity reaction”, JSPS KAKENHI Grant Number 15K18903, Grant-in-Aid for Young Scientists (B) – Japan and MEXT – Supported Program for the Strategic Research Foundation at Private Universities (2013–2017) – Japan.2015年度関西大学若手研究者育成経
Efficient Synthesis of t-Butyl 3-Alkyl-N-hydroxyoxindole-3-carboxylates from Di-t-butyl 2-nitrophenylmalonates
Process Development for the Synthesis of a Selective M<sub>1</sub> and M<sub>4</sub> Muscarinic Acetylcholine Receptors Agonist
A practical and chromatography-free
synthetic process to selective
M<sub>1</sub> and M<sub>4</sub> muscarinic acetylcholine receptors
agonist was developed and demonstrated on a several hundred gram scale.
The key feature of this route is <i>N,N</i>-dimethylcarbamoylation
of the anilinic nitrogen on the spiro 7-azaindoline structure via
intermolecular migration of the <i>N,N</i>-dimethylcarbamoyl
group. The resulting compound <b>1</b> was prepared in 43% overall
yield with a chemical purity >99% via six steps starting with (2-chloropyridin-3-yl)acetonitrile
Asymmetric Synthesis of t-Butyl 3-Alkyl-oxindole-3-carboxylates via Chiral Phosphoric Acid Catalyzed Desymmetrization of Di-t-butyl 2-Alkyl-2-(2-aminophenyl)malonates
Cyclic Model for the Asymmetric Conjugate Addition of Organolithiums with Enoates
The chiral diether ligand controlled asymmetric conjugate addition of organolithiums to nona-2, 7-dienedioates preferentially proceeds via the s-cis conformation with coordination of the carbonyl oxygen atom to the lithium to give a lithium E-enolate intermediate. Subsequent intramolecular conjugate addition of the enolate also proceeds via a cyclic transition state involving the lithium and the s-cis-enoate, resulting in trans, trans-trisubstituted cyclohexanes with high enantiomeric excesses and yields
Asymmetric Synthesis of the Carbon-14-Labeled Selective Glucocorticoid Receptor Modulator using Cinchona Alkaloid Catalyzed Addition of 6-Bromoindole to Ethyl Trifluoropyruvate
molecule