Difficulty as an Affective Factor: Does \u27No Error\u27 Mean \u27No Difficulty\u27?

This paper examines learners\u27 perception of difficulty, a factor affecting motivation. Despite the fact that researchers and teachers would probably agree on the need to understand learner difficulty, the phenomenon of learners finding language learning difficult has not received much attention either in the research literature or in the professional literature of classroom language learning and teaching. This is mainly due to the assumption that errors adequately reflect difficulty. This paper discusses how learner difficulty is related to learner performance as well as how difficulty is perceived by individual learners, and considers the implications for task motivation

コミュニケーション ノウリョク ノ イクセイ オ メザシタ エイゴ シドウ

Fostering learner communicative competence has become a major objective of English language education in Japan. From a team-teaching perspective, this paper discusses ways in which this objective can be better achieved. It argues that team-teaching should be reformulated so as to promote interaction and authentic communication, and realize an input-and output-rich environment in the classroom. It also demonstrates that a re-interpretation of the nature of ALTs could shed light on a new aspect of their role in what I have called \u27content-based team-teaching\u27

Culture temperature affects human chondrocyte messenger RNA expression in monolayer and pellet culture systems

Cell-based therapy has been explored for articular cartilage regeneration. Autologous chondrocyte implantation is a promising cell-based technique for repairing articular cartilage defects. However, there are several issues such as chondrocyte de-differentiation. While numerous studies have been designed to overcome some of these issues, only a few have focused on the thermal environment that can affect chondrocyte metabolism and phenotype. In this study, the effects of different culture temperatures on human chondrocyte metabolism- and phenotype-related gene expression were investigated in 2D and 3D environments. Human chondrocytes were cultured in a monolayer or in a pellet culture system at three different culture temperatures (32° C, 37° C, and 41° C) for 3 days. The results showed that the total RNA level, normalized to the threshold cycle value of internal reference genes, was higher at lower temperatures in both culture systems. Glyceraldehyde-3- phosphate dehydrogenase (GAPDH) and citrate synthase (CS), which are involved in glycolysis and the citric acid cycle, respectively, were expressed at similar levels at 32° C and 37° C in pellet cultures, but the levels were significantly lower at 41° C. Expression of the chondrogenic markers, collagen type IIA1 (COL2A1) and aggrecan (ACAN), was higher at 37° C than at 32° C and 41° C in both culture systems. However, this phenomenon did not coincide with SRY (sex-determining region Y)-box 9 (SOX9), which is a fundamental transcription factor for chondrogenesis, indicating that a SOX9-independent pathway might be involved in this phenomenon. In conclusion, the expression of chondrocyte metabolism-related genes at 32° C was maintained or enhanced compared to that at 37° C. However, chondrogenesis-related genes were further induced at 37° C in both culture systems. Therefore, manipulating the culture temperature may be an advantageous approach for regulating human chondrocyte metabolic activity and chondrogenesis

The effects of short-term hypoxia on human mesenchymal stem cell proliferation, viability and p16(INK4A) mRNA expression: Investigation using a simple hypoxic culture system with a deoxidizing agent

A hypoxic environment is thought to be important for the maintenance of stemness and suppressing cell senescence, in stem cells. Therefore, a hypoxic condition is induced during cell expansion and/or induction of intended differentiation. However, the induction of these conditions requires a specially equipped hypoxia chamber and expensive gas mixtures, which are expensive and space-consuming. Owing to these restrictions, appropriate hypoxic conditions cannot be provided during cell transportation, which is increasingly required for regenerative medicine. Hence, a simple and economical culture system is required. The purpose of this study was to investigate the effects of short-term hypoxic conditions on human mesenchymal stem cell (MSC) proliferation, viability, and senescence, utilizing the CulturePal system (CulturePal-Zero and CulturePal-Five), a novel and simple hypoxic culture system with a built-in deoxidizing agent. The O₂ concentration in the CulturePal-Zero was observed to reduce to <0.1% within 1 h, and to 5% within 24 h in the CulturePal-Five system. Cell proliferation under these hypoxic conditions showed a sharp increase at 5% O₂ concentration, and no noticeable cell death was observed even at severe hypoxic conditions (<0.1% O₂) up to 72h. The p16INK4A (cell senescence marker) mRNA expression was retained under hypoxic conditions up to 72h, but it was up-regulated under normoxic conditions. Interestingly, the p16INK4A expression altered proportionately to the O₂ concentration. These results indicated that the short-term hypoxic condition, at an approximate O₂ concentration of 5%, would be suitable for promoting cell proliferation and repressing cell senescence, without aggravating the MSC viability. Therefore, the CulturePal systems may be suitable for providing an appropriate hypoxic condition in stem cell research and transportation

Contributions of biarticular myogenic components to the limitation of the range of motion after immobilization of rat knee joint

BACKGROUND: Muscle atrophy caused by immobilization in the shortened position is characterized by a decrease in the size or cross-sectional area (CSA) of myofibers and decreased muscle length. Few studies have addressed the relationship between limitation of the range of motion (ROM) and the changes in CSA specifically in biarticular muscles after atrophy because of immobilization. We aimed to determine the contribution of 2 distinct muscle groups, the biarticular muscles of the post thigh (PT) and those of the post leg (PL), to the limitation of ROM as well as changes in the myofiber CSAs after joint immobilization surgery. METHODS: Male Wistar rats (n = 40) were randomly divided into experimental and control groups. In the experimental group, the left knee was surgically immobilized by external fixation for 1, 2, 4, 8, or 16 weeks (n = 5 each) and sham surgery was performed on the right knee. The rats in the control groups (n = 3 per time point) did not undergo surgery. After the indicated immobilization periods, myotomy of the PT or PL biarticular muscles was performed and the ROM was measured. The hamstrings and gastrocnemius muscles from the animals operated for 1 or 16 weeks were subjected to morphological analysis. RESULTS: In immobilized knees, the relative contribution of the PT biarticular myogenic components to the total restriction reached 80% throughout the first 4 weeks and decreased thereafter. The relative contribution of the PL biarticular myogenic components remained <20% throughout the immobilization period. The ratio of the myofiber CSA of the immobilized to that of the sham-operated knees was significantly lower at 16 weeks after surgery than at 1 week after surgery only in the hamstrings. CONCLUSIONS: The relative contribution of the PT and PL components to myogenic contracture did not significantly change during the experimental period. However, the ratio of hamstrings CSAs to the sham side was larger than the ratio of medial gastrocnemius CSAs to the sham side after complete atrophy because of immobilization

Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load

BACKGROUND: The differences of mechanical and histological properties between cartilage covered by menisci and uncovered by menisci may contribute to the osteoarthritis after meniscectomy and these differences are not fully understood. The purpose of this study is to investigate potential differences in the mechanical and histological properties, and in particular the collagen architecture, of the superficial cartilage layer and subchondral bone between regions covered and uncovered by menisci using immature knee. METHODS: Osteochondral plugs were obtained from porcine tibial cartilage that was either covered or uncovered by menisci. Investigation of the thickness, mechanical properties, histology, and water content of the cartilage as well as micro-computed tomography analysis of the subchondral bone was performed to compare these regions. Collagen architecture was also assessed by using scanning electron microscopy. RESULTS: Compared to the cartilage uncovered by menisci, that covered by menisci was thinner and showed a higher deformity to compression loading and higher water content. In the superficial layer of cartilage in the uncovered regions, collagen fibers showed high density, whereas they showed low density in covered regions. Furthermore, subchondral bone architecture varied between the 2 regions, and showed low bone density in covered regions. CONCLUSIONS: Cartilage covered by menisci differed from that uncovered in both its mechanical and histological properties, especially with regards to the density of the superficial collagen layer. These regional differences may be related to local mechanical environment in normal condition and indicate that cartilage covered by menisci is tightly guarded by menisci from extreme mechanical loading. Our results indicate that immature cartilage degeneration and subchondral microfracture may occur easily to extreme direct mechanical loading in covered region after meniscectomy

The Effect of Exercise on the Early Stages of Mesenchymal Stromal Cell-Induced Cartilage Repair in a Rat Osteochondral Defect Model

The repair of articular cartilage is challenging owing to the restriction in the ability of articular cartilage to repair itself. Therefore, cell supplementation therapy is possible cartilage repair method. However, few studies have verified the efficacy and safety of cell supplementation therapy. The current study assessed the effect of exercise on early the phase of cartilage repair following cell supplementation utilizing mesenchymal stromal cell (MSC) intra-articular injection. An osteochondral defect was created on the femoral grooves bilaterally of Wistar rats. Mesenchymal stromal cells that were obtained from male Wistar rats were cultured in monolayer. After 4 weeks, MSCs were injected into the right knee joint and the rats were randomized into an exercise or no-exercise intervention group. The femurs were divided as follows: C group (no exercise without MSC injection); E group (exercise without MSC injection); M group (no exercise with MSC injection); and ME group (exercise with MSC injection). At 2, 4, and 8 weeks after the injection, the femurs were sectioned and histologically graded using the Wakitani cartilage repair scoring system. At 2 weeks after the injection, the total histological scores of the M and ME groups improved significantly compared with those of the C group. Four weeks after the injection, the scores of both the M and ME groups improved significantly. Additionally, the scores in the ME group showed a significant improvement compared to those in the M group. The improvement in the scores of the E, M, and ME groups at 8 weeks were not significantly different. The findings indicate that exercise may enhance cartilage repair after an MSC intra-articular injection. This study highlights the importance of exercise following cell transplantation therapy

Decision-making Concerning Individual Learner Differences in the EFL Classroom : Perspectives of Japanese Junior High School Teachers

Over the last few decades there have been considerable advances in the literature on individual learner differences in foreign or second language (L2) learning. Research shows in what ways learners are different and how these differences can affect their success in L2 learning. While admitting the significance of individual learner differences, few attempts have been made to investigate how teachers actually cope with such differences in the classroom. It is assumed that the way teachers view these individual learner differences may affect their decision-making in planning their approach to particular lessons, the level of language or teaching/learning material to be used, or the design of tasks or tests, which may in turn determine the final overall success of the lessons. The focus of the present paper is on one aspect of this issue; specifically Japanese teachers\u27 views about their classes. It reports preliminary findings of the English teachers\u27 perceptions of their EFL classes at the junior high school level. The data shows that Japanese EFL teachers are more concerned with relatively low achieving students and assumes that their lessons are planned accordingly. The data further suggests that the teachers\u27 attitudes may be affected by the fact that junior high school education is part of the compulsory education system in Japan where credence is given to the belief that it is more important not to leave any low achieving student behind rather than to help high achieving students

Students' Perceptions of Difficulties with Academic Writing: A Report from Kyoto University Academic Writing Courses

Academic writing is one of the keys to success in students' academic lives, yet one of the most difficult skills to learn. In 2006, Kyoto University adopted a new English curriculum in Liberal Arts and General Education requiring all first-year and second-year students to enroll in academic English courses. This paper aims to identify and report the difficulties students face in the process of academic writing in their transition from high school to their first and second year at the university. The study discussed in this paper was conducted in two first-year academic writing courses and one second-year academic writing course at Kyoto University in spring 2007. At the end of the term, the students completed a survey about their perceptions of difficulties with the content of the course. The first-year students reported most difficulty with adapting to the transition from high school practical English (English for General Purposes) to university academic English (English for Academic Purposes), especially with writing in a formal style and thinking about the rules of academic writing. The second-year students reported most difficulty with producing a coherent academic paragraph with no redundant sentences. Pedagogical implications are discussed

Understanding Students' Perceptions of Difficulty with Academic Writing for Teacher Development: A Case Study of the University of Tokyo Writing Program

This paper examines students' perceptions of difficulty with academic writing in English. Writing seems to be one of the most important, yet difficult, academic skills for university students to learn. This is particularly the case with foreign or second language (L2) learners. While it is often claimed that the understanding of students' difficulties is a necessary condition for becoming an ideal L2 teacher, few implications have been offered for teacher development in the literature of academic writing. The present study aims to deal with this issue using data obtained from 95 first-year Japanese university students. The results of the study show that the students tended to perceive academic writing to be difficult. In particular, they perceived language-related components of academic writing to be more difficult than structure/content-related components. Moreover, it was found that a great number of students expressed a high degree of difficulty with research design. This paper concludes with a discussion of some of the implications for teacher development