Nitraria komarovii Iljin & Lava ex Bobrov (Nitrariaceae), a new record for the flora of Kazakhstan

Nitraria komarovii Iljin & Lava ex Bobrov is newly reported for the flora of Kazakhstan. The two new records extend the range of this species eastward from its previously known range in Azerbaijan and Turkmenistan. A full description of N. komarovii is given for the first time, along with illustrations, notes on its taxonomy, and a distribution map. Nitraria komarovii is most similar to N. schoberi L., but differs in its habit, narrower and longer, linear-spatulate, greenish-yellow leaves, which gradually narrow to the base, more subtle inflorescences, size of the stone, petal, stamen, and pistil, and color of the fruit

Variation of nuclear DNA content in seeds of Nitraria schoberi L.

For the first time are search of nuclear DNA relative content in Nitraria schoberi L. seeds from 15 natural populations of Siberia, the Crimea, Kazakhstan, and Tajikistan has been conducted by the method of flow cytometry. High intra-and interpopulational variations of nuclear DNA content - 2,93-3,39 pg, at average value - 3,22±0,108 pg is revealed

Possibilities of using the HPLC method in the taxonomy of the genus

The paper shows composition and content of phenolic compounds in leaves of 4 Nitraria L. species from 58 populations of Russia, Kazakhstan, and Tajikistan studied with high-performance liquid chromatography(HPLC). The investigation has revealed 27 compounds of phenolic nature: the maximum number (18 components) is detected in leaves of N. sibirica Pall. from Kazakhstan (the Karatal river valley), 16-17 components – in plants from three populations of Siberia, the minimum (6 components) – in leaves of N. komaroviiilljin & Lava ex Bobrov. The analysis has identified hyperoside (quercetin O-glycoside), narcissin (isoramnetin O-glycoside), quercetin (flavonol) and luteolin (flavon). The studied plants accumulate a generous quantity of phenolic compounds. Their content reaches 4.64% in leaves of N. sibirica, 3.11% – in N. schoberi L., up to 3.96% – in N. komarovii. The research results allow speaking about the species-specific composition and content of phenolic compounds of N. sibirica, N. schoberi and N. komarovii. The component composition is weaker in extracts of N. komarovii leaves, but there is a higher content of total phenolic compounds compared to N. schoberi plants. N. pamirica L. Vassil sample is close to N. schoberi on multidimensional analysis of the phenolic compound composition and content

Variation of nuclear DNA content in seeds of

For the first time are search of nuclear DNA relative content in Nitraria schoberi L. seeds from 15 natural populations of Siberia, the Crimea, Kazakhstan, and Tajikistan has been conducted by the method of flow cytometry. High intra-and interpopulational variations of nuclear DNA content - 2,93-3,39 pg, at average value - 3,22±0,108 pg is revealed

Using the ITS2 transcribed spacer of nuclear ribosomal DNA to identify species of the genus Nitraria

Intragenomic polymorphism of ITS2 of nuclear ribosomal DNA sequences was analysed in 30 samples belonging to Nitraria schoberi, N. sibirica. The nucleotide variability of the ITS2 region was detected in the studied Nitraria species as single-nucleotide substitutions (mainly transitions) and single-nucleotide deletion. Five ribotypes of Nitraria were identified in Russia, Kazakhstan and Tajikistan. These ribotypes formed two haplogroups that belong to the species N. schoberi and N. sibirica, respectively. The high importance of the discriminatory role of the ITS2 spacer in identifying species of the genus Nitraria is confirmed