MEMS flow sensors for nano-fluidic applications

This paper presents micromachined thermal sensors for measuring liquid flow rates in the nanoliter-per-minute range. The sensors use a boron-doped polysilicon thinfilm heater that is embedded in the silicon nitride wall of a microchannel. The boron doping is chosen to increase the heater’s temperature coefficient of resistance within tolerable noise limits, and the microchannel is suspended from the substrate to improve thermal isolation. The sensors have demonstrated a flow rate resolution below 10 nL/min, as well as the capability for detecting micro bubbles in the liquid. Heat transfer simulation has also been performed to explain the sensor operation and yielded good agreement with experimental data

Ergodicity for the GI/G/1GI/G/1-type Markov Chain

Ergodicity is a fundamental issue for a stochastic process. In this paper, we refine results on ergodicity for a general type of Markov chain to a specific type or the $GI/G/1$-type Markov chain, which has many interesting and important applications in various areas. It is of interest to obtain conditions in terms of system parameters or the given information about the process, under which the chain has various ergodic properties. Specifically, we provide necessary and sufficient conditions for geometric, strong and polynomial ergodicity, respectively.Comment: 16 page

Micromachined membrane particle filters

We report here several particle membrane filters (8 x 8 mm^2) with circular, hexagonal and rectangular through holes. By varying hole dimensions from 6 to 12 pm, opening factors from 4 to 45 % are achieved. In order to improve the filter robustness, a composite silicon nitride/Parylene membrane technology is developed. More importantly, fluid dynamic performance of the filters is also studied by both experiments and numerical simulations. It is found that the gaseous flow through the filters depends strongly on opening factors, and the measured pressure drops are much lower than that from numerical simulation using the Navier-Stokes equation. Interestingly, surface velocity slip can only account for a minor part of the discrepancy. This suggests that a very interesting topic for micro fluid mechanics research is identified

Effects of Repeated Central Administration of Endothelin Type A Receptor Antagonist on the Development of Neuropathic Pain in Rats

published_or_final_versio

Normalization Perturbation: A Simple Domain Generalization Method for Real-World Domain Shifts

Improving model's generalizability against domain shifts is crucial,especially for safety-critical applications such as autonomous driving.Real-world domain styles can vary substantially due to environment changes andsensor noises, but deep models only know the training domain style. Such domainstyle gap impedes model generalization on diverse real-world domains. Ourproposed Normalization Perturbation (NP) can effectively overcome this domainstyle overfitting problem. We observe that this problem is mainly caused by thebiased distribution of low-level features learned in shallow CNN layers. Thus,we propose to perturb the channel statistics of source domain features tosynthesize various latent styles, so that the trained deep model can perceivediverse potential domains and generalizes well even without observations oftarget domain data in training. We further explore the style-sensitive channelsfor effective style synthesis. Normalization Perturbation only relies on asingle source domain and is surprisingly effective and extremely easy toimplement. Extensive experiments verify the effectiveness of our method forgeneralizing models under real-world domain shifts.<br

Preventive Treatment with Ketamine attenuates the ischaemia-reperfusion response in a chronic postichaemia pain model

published_or_final_versio

Genome-scale analysis and comparison of gene expression profiles in developing and germinated pollen in Oryza sativa

<p>Abstract</p> <p>Background</p> <p>Pollen development from the microspore involves a series of coordinated cellular events, and the resulting mature pollen has a specialized function to quickly germinate, produce a polar-growth pollen tube derived from the vegetative cell, and deliver two sperm cells into the embryo sac for double fertilization. The gene expression profiles of developing and germinated pollen have been characterised by use of the eudicot model plant <it>Arabidopsis</it>. Rice, one of the most important cereal crops, has been used as an excellent monocot model. A comprehensive analysis of transcriptome profiles of developing and germinated pollen in rice is important to understand the conserved and diverse mechanism underlying pollen development and germination in eudicots and monocots.</p> <p>Results</p> <p>We used Affymetrix GeneChip^®Rice Genome Array to comprehensively analyzed the dynamic changes in the transcriptomes of rice pollen at five sequential developmental stages from microspores to germinated pollen. Among the 51,279 transcripts on the array, we found 25,062 pollen-preferential transcripts, among which 2,203 were development stage-enriched. The diversity of transcripts decreased greatly from microspores to mature and germinated pollen, whereas the number of stage-enriched transcripts displayed a "U-type" change, with the lowest at the bicellular pollen stage; and a transition of overrepresented stage-enriched transcript groups associated with different functional categories, which indicates a shift in gene expression program at the bicellular pollen stage. About 54% of the now-annotated rice F-box protein genes were expressed preferentially in pollen. The transcriptome profile of germinated pollen was significantly and positively correlated with that of mature pollen. Analysis of expression profiles and coexpressed features of the pollen-preferential transcripts related to cell cycle, transcription, the ubiquitin/26S proteasome system, phytohormone signalling, the kinase system and defense/stress response revealed five expression patterns, which are compatible with changes in major cellular events during pollen development and germination. A comparison of pollen transcriptomes between rice and <it>Arabidopsis </it>revealed that 56.6% of the rice pollen preferential genes had homologs in <it>Arabidopsis </it>genome, but 63.4% of these homologs were expressed, with a small proportion being expressed preferentially, in <it>Arabidopsis </it>pollen. Rice and <it>Arabidopsis </it>pollen had non-conservative transcription factors each.</p> <p>Conclusions</p> <p>Our results demonstrated that rice pollen expressed a set of reduced but specific transcripts in comparison with vegetative tissues, and the number of stage-enriched transcripts displayed a "U-type" change during pollen development, with the lowest at the bicellular pollen stage. These features are conserved in rice and <it>Arabidopsis</it>. The shift in gene expression program at the bicellular pollen stage may be important to the transition from earlier cell division to later pollen maturity. Pollen at maturity pre-synthesized transcripts needed for germination and early pollen tube growth. The transcription regulation associated with pollen development would have divergence between the two species. Our results also provide novel insights into the molecular program and key components of the regulatory network regulating pollen development and germination.</p

Central administration of C-x-C chemokine receptor type 4 antagonist alleviates the development and maintenance of peripheral neuropathic pain in mice

Aim To explore the roles of C-X-C chemokine receptor type 4 (CXCR4) in spinal processing of neuropathic pain at the central nervous system (CNS). Methods Peripheral neuropathic pain (PNP) induced by partial sciatic nerve ligation (pSNL) model was assessed in mice. Effects of a single intrathecal (central) administration of AMD3100 (intrathecal AMD3100), a CXCR4 antagonist, on pain behavior and pain-related spinal pathways and molecules in the L3-L5 spinal cord segment was studied compare to saline treatment. Results Rotarod test showed that intrathecal AMD3100 did not impair mice motor function. In pSNL-induced mice, intrathecal AMD3100 delayed the development of mechanical allodynia and reversed the established mechanical allodynia in a dose-dependent way. Moreover, intrathecal AMD3100 downregulated the activation of JNK1 and p38 pathways and the protein expression of p65 as assessed by western blotting. Real-time PCR test also demonstrated that substance P mRNA was decreased, while adrenomedullin and intercellular adhesion molecule mRNA was increased following AMD3100 treatment. Conclusion Our results suggest that central (spinal) CXCR4 is involved in the development and maintenance of PNP and the regulation of multiple spinal molecular events under pain condition, implicating that CXCR4 would potentially be a therapeutic target for chronic neuropathic pain.published_or_final_versio