1 research outputs found
Evaluation of the Coagulant Effect of the Zanjani and Latifi Viper Venom Endemic in Iran
The venom of the viper is very important in pharmaceutical usage, such as in the process
of coagulation during medical care. This study aims to evaluate the coagulant factor of
Latifi and Zanjani viper venom. In the current study, after electrophoresis of proteins
found in viper venom, all of the thick and strong bands of proteins were isolated and
prepared for examination of coagulation characteristics, like pro-thrombin time (PTT)
and active partial thromboplastic time (APTT), followed by further study by mass
spectroscopy. In this way, 11 bands of proteins were recognized in sodium dodecyl
sulphate polyacrylamide gel electrophoresis (SDS-PAGE). After PT and APTT tests, one
common band in 26 KDa could lead to coagulation of blood plasma in less than one
second. Mass spectroscopy identified this band as serine protease isoform4. The results
confirmed the coagulation effect of a 26 KDa protein fraction of venom from Latifi and
Zanjani vipers