Practical Preparation of Epilactose Produced with Cellobiose 2-Epimerase from Ruminococcus albus NE1

A practical purification method for a non-digestible disaccharide, epilactose (4-O-beta-galactosyl-D-mannose), was established. Epilactose was synthesized from lactose with cellobiose 2-epimerase and purified by the following procedure: (i) removal of lactose by crystallization, (ii) hydrolysis of lactose by beta-galactosidase, (iii) digestion of monosaccharides by yeast, and (iv) column chromatography with Na-form cation exchange resin. Epilactose of 91.1% purity was recovered at 42.5% yield

Auroral O+ 732/733 nm emission and its relation to ion upflow

Observations of auroral oxygen ion emission at 732/733 nm were made at the Aurora station in Longyearbyen (78.2.N, 16.3.E; lm=74.9.) using an all-sky spectrograph (ASG) during the winter season of 2000/2001. A statistical analysis showed that the highest occurrence of oxygen ion auroras at Longyearbyen was seen in the dayside of the 09-12MLT region; the intensities of these auroras were also larger than those on the night side. To study the mechanism of ion up゜ow in the polar ionosphere, ASG data obtained on December 7, 2000, was analyzed together with simultaneous ionospheric data obtained by EISCAT Svalbard radar (ESR). Enhancements of electron temperature and ion upward velocity were associated with an increase in the auroral OII intensity at the magnetic zenith. This result suggests that an ambipolar electric field associated with electron temperature enhancement caused by soft electron precipitation may be involved in the mechanisms that drive ionospheric ions upward

Selective loss of Purkinje cells in a patient with anti-gliadin-antibody-positive autoimmune cerebellar ataxia

The patient was an 84-year-old woman who had the onset of truncal ataxia at age 77 and a history of Basedow's disease. Her ataxic gait gradually deteriorated. She could not walk without support at age 81 and she was admitted to our hospital at age 83. Gaze-evoked nystagmus and dysarthria were observed. Mild ataxia was observed in all limbs. Her deep tendon reflex and sense of position were normal. IgA anti-gliadin antibody, IgG anti-gliadin antibody, anti-SS-A/Ro antibody, anti-SS-B/La antibody and anti-TPO antibody were positive. A conventional brain MRI did not show obvious cerebellar atrophy. However, MRI voxel based morphometry (VBM) and SPECT-eZIS revealed cortical cerebellar atrophy and reduced cerebellar blood flow. IVIg treatment was performed and was moderately effective. After her death at age 85, the patient was autopsied. Neuropathological findings were as follows: selective loss of Purkinje cells; no apparent degenerative change in the efferent pathways, such as the dentate nuclei or vestibular nuclei; no prominent inflammatory reaction. From these findings, we diagnosed this case as autoimmune cerebellar atrophy associated with gluten ataxia. All 3 autopsies previously reported on gluten ataxia have noted infiltration of inflammatory cells in the cerebellum

A new meridian imaging spectrogarph for the auroral spectroscopy

Spectroscopic and monochromatic imaging observations of emissions in the upper atmosphere are mutually complementary. A meridian imaging auroral spectrograph (ASG) that can measure a spectrum in the visible region along a meridian has been developed for research on the auroral physics and the polar upper-atmosphere dynamics. Combination of a fast optical system inherited from a monochromatic all-sky imager, a grism as a dispersive element, and a cooled CCD camera has enabled a wide field-of-view of 180° along a meridian, spectral coverage of 420-730 nm, spectral resolution of 1.5-2.0 nm, and high sensitivity to be obtained. The absolute sensitivity over a full-image field was calibrated using a National Institute of Standards and Technology (NIST) traceable integrating sphere and determined to be 0.06 cts/s/R at a wavelength of 560 nm at the zenith. The ASG was installed at Longyearbyen in March 2000, and routine operation was started in the 2000/2001 winter season. An example of an auroral spectral image is presented in this report to demonstrate the performance of the ASG

Current status of ground-based optical observations for short-wavelength infrared aurora and airglow emissions in Northern Europe

The Tenth Symposium on Polar Science/Ordinary sessions: [OS] Space and upper atmospheric sciences, Wed. 4 Dec. /Entrance Hall (1st floor) at National Institute of Polar Research (NIPR

Environmental Kuznets curve on water pollution in Chinese provinces

This study, focusing on the water pollutions in terms of chemical oxygen demand (COD) and ammonia nitrogen by industrial and household discharges in Chinese provinces, investigates the contribution of capacity shortage for pollution control to the provincial pollution levels, by conducting a factor analysis to the heterogeneity of provincial pollutions under the environmental Kuznets curve (EKC) framework. The study’s contribution to the literature lies in its framework of analyzing the heterogeneity of Chinese provinces’ EKCs in terms of their positions (not their shapes) by using a fixed-effect model to extract the province-specific pollution effects. The main finding of this study is that the capacity shortage for pollution control accounts for around 30% as a pollution factor of industrial COD and ammonia nitrogen, and accounts for around 60% and 80% as a pollution factor of household COD and ammonia nitrogen, respectively. It suggests that China has still much policy space and room to mitigate the water pollutions, by building the capacity for pollution control through developing human resources and training them

Expression of angiopoietin-like 4 in human gastric cancer: ANGPTL4 promotes venous invasion

There is strong evidence that the angiopoietin family is involved in the regulation of tumour progression, cellular growth and differentiation. Recently, it has been reported that angiopoietin-like 4 (ANGPTL4) in cancer cell promotes the metastatic process by increasing vascular permeability. To elucidate ANGPTL4 expression and its association with clinicopathological factors and prognosis in human gastric adenocarcinomas, we examined 103 cases of surgically-resected human gastric adenocarcinoma by immunohistochemistry. Among 103 cases of adenocarcinoma, 38 cases (36.9%) showed positive staining in the cytoplasm of the carcinoma cells for ANGPTL4. Histologically, papillary and mucinous adenocarcinomas showed relatively high expression of ANGPTL4 (60 and 60%, respectively). The expression of ANGPTL4 was correlated with the depth of tumour invasion (p<0.005), lymph node metastasis (p<0.001), venous invasion (p<0.00005) and TNM stage (p<0.001) in the total carcinoma. In univariate survival analysis, ANGPTL4 expression was not associated with the overall survival. RT-PCR or Western blot analysis showed the expression of mRNA or protein of ANGPTL4 in all four surgically-resected samples and all four cell lines of human gastric adenocarcinoma. ANGPTL4 expression was correlated with several clinicopathological factors, especially venous invasion. These findings suggest that the ANGPTL4 is one of the factors involved in the progression of human gastric cancer