Falta de estructura genética en las poblaciones chipriotas de Alectoris chukar (Aves, Galliformes), deducida de los datos de secuenciación del ADNmt

The Chukar (Alectoris chukar cypriotes) is the most common game bird in Cyprus. Since 1990 the Cypriot Government has established a restocking program with captive–reared birds. However, this program has not been guaranteed by checking the genetic nature of wild and farmed samples, either in the areas controlled by the Cypriot Government or in northern Cyprus. The sequencing of both Cytochrome–b and Control Region of the mitochondrial DNA was carried out for 61 Cypriot representatives and 14 specimens of the same subspecies from Crete and Israel. Only the A. chukar maternal lineage was found. A partitioning of Cypriot specimens among different clades was not reliably supported, whereas robust bootstrap values weighted for an evolutionary divergence between Cypriot and Cretan Chukars. An overall genetic homogeny of the Cypriot populations was disclosed, whatever their status (captive vs. wild stocks) and origin (Government controlled vs. occupied areas) would be, a higher nucleotide diversity of the wild vs. captive representatives notwithstanding. Key words: Chukar, Control Region, Cytochrome–b, Genetic diversity, mtDNA, Partridges.Una subespecie de la perdiz chucar (Alectoris chukar cypriotes) es el ave de caza más común de Chipre. A partir de 1990 el gobierno chipriota estableció un programa de repoblación utilizando aves criadas en cautividad. No obstante, dicho programa no ha sido avalado mediante la comprobación de la naturaleza genética de muestras tanto de ejemplares salvajes como de granja, ni en las zonas controladas por el gobierno chipriota ni en el norte de Chipre. Se ha llevado a cabo la secuenciación del citocromo-b y de la región de control del ADN mitocondrial de 61 ejemplares chipriotas y de 14 especimenes de la misma subespecie de Creta y de Israel. Sólo se encontró el linaje materno de A. chukar. No se pudo demostrar con fiabilidad el reparto de los especimenes chipriotas en distintos clados, mientras que unos valores bootstrap muy consistentes sustentaban una divergencia evolutiva entre las perdices chucar chipriotas y cretenses. Se reveló la existencia de una homogeneidad genética en las poblaciones chipriotas, cualquiera que fuera su estatus (linajes cautivos frente a salvajes) o su origen (zonas controladas por el gobierno frente a zonas ocupadas), por más que se diera una mayor diversidad de nucleótidos de los ejemplares salvajes frente a los cautivos. Palabras clave: Perdiz chucar, Región de control, Citocromo-b, Diversidad genética, ADNmt, Perdices

Inactivation of eimeria Oocysts in aqueous solution by a dielectric barrier discharge plasma in contact with liquid

This study presents a novel technique to inactivate coccidian oocysts in an aqueous solution. The technique consists of treating the contaminated liquid by using an atmospher- ic-pressure air dielectric barrier discharge (DBD) plasma in contact with it. Many experiments in several operating conditions were performed. The discharge was supplied by sinusoidal and nanosecond-pulsed voltages with a constant average power of ~7 W in both cases. Biological effects due to the plasma were investigated by performing tests with increasing treatment time. A sudden ~40% drop in the number of survived oocysts was reached in 4 min and a two-fold reduction was detected after 12 min of exposure. No significant differences in the biocidal efficacy were detected between the AC-driven and the nanosecond-pulsed discharge. Chi-squared statistical analysis on the treated samples showed significant statistical difference (with a statistical significance P value parameter less than 0.01) and nonrandomness warranty of the results, opening interesting scenarios for fimire developments

Intracrine endorphinergic systems in modulation of myocardial differentiation

A wide variety of peptides not only interact with the cell surface, but govern complex signaling from inside the cell. This has been referred to as an "intracrine" action, and the orchestrating molecules as "intracrines". Here, we review the intracrine action of dynorphin B, a bioactive end-product of the prodynorphin gene, on nuclear opioid receptors and nuclear protein kinase C signaling to stimulate the transcription of a gene program of cardiogenesis. The ability of intracrine dynorphin B to prime the transcription of its own coding gene in isolated nuclei is discussed as a feed-forward loop of gene expression amplification and synchronization. We describe the role of hyaluronan mixed esters of butyric and retinoic acids as synthetic intracrines, controlling prodynorphin gene expression, cardiogenesis, and cardiac repair. We also discuss the increase in prodynorphin gene transcription and intracellular dynorphin B afforded by electromagnetic fields in stem cells, as a mechanism of cardiogenic signaling and enhancement in the yield of stem cell-derived cardiomyocytes. We underline the possibility of using the diffusive features of physical energies to modulate intracrinergic systems without the needs of viral vector-mediated gene transfer technologies, and prompt the exploration of this hypothesis in the near future

Characterization of a dielectric barrier discharge in contact with liquid and producing a plasma activated water

In this work a low-temperature plasma source for the generation of plasma activated water (PAW) is developed and characterized. The plasma reactor was operated by means of an atmospheric-pressure air dielectric barrier discharge (DBD). The plasma generated is in contact with the water surface and is able to chemically activate the liquid medium. Electrodes were supplied by both sinusoidal and nanosecond-pulsed voltage waveforms. Treatment times were varied from 2 to 12 min to increase the energy dose released to the water by the DBD plasma. The physics of the discharge was studied by means of electrical, spectroscopic and imaging diagnostics. The interaction between the plasma and the liquid was investigated as well. Temperature and composition of the treated water were detected. Images of the discharges showed a filamentary behaviour in the sinusoidal case and a more homogeneous behaviour in the nanosecond-pulsed one. The images and the electrical measurements allowed to evaluate an average electron number density of about 4 7 1019 and 6 7 1017 m-3 for the sinusoidal and nanosecond-pulsed discharges respectively. Electron temperatures in the range of 2.1\uf72.6 eV were measured by using spectroscopic diagnostics. Rotational temperatures in the range of 318-475 K were estimated by fitting synthetic spectra with the measured ones. Water temperature and pH level did not change significantly after the exposure to the DBD plasma. The production of ozone and hydrogen peroxide within the water was enhanced by increasing the plasma treatment time and the energy dose. Numerical simulations of the nanosecond-pulsed discharge were performed by using a self-consistent coupling of state-to-state kinetics of the air mixture with the Boltzmann equation of free electron kinetics. Temporal evolution of the electron energy distribution function shows departure from the Maxwellian distribution especially during the afterglow phase of the discharge. When limited deviations from Maxwellian distribution were observed, calculated electron temperature is in good agreement with the one measured by means of spectroscopic diagnostics. Computed temporal evolution of the energy delivered to the discharge is comparable with the one obtained from electrical measurements. The electrical discharges supplied by both voltage waveforms produce plasma activated water with negligible thermal effects and pH variations

Lack of genetic structure of Cypriot Alectoris chukar (Aves, Galliformes) populations as inferred from mtDNA sequencing data

The Chukar (Alectoris chukar cypriotes) is the most common game bird in Cyprus. Since 1990 the Cypriot Government has established a restocking program with captive–reared birds. However, this program has not been guaranteed by checking the genetic nature of wild and farmed samples, either in the areas controlled by the Cypriot Government or in northern Cyprus. The sequencing of both Cytochrome–b and Control Region of the mitochondrial DNA was carried out for 61 Cypriot representatives and 14 specimens of the same subspecies from Crete and Israel. Only the A. chukar maternal lineage was found. A partitioning of Cypriot specimens among different clades was not reliably supported, whereas robust bootstrap values weighted for an evolutionary divergence between Cypriot and Cretan Chukars. An overall genetic homogeny of the Cypriot populations was disclosed, whatever their status (captive vs. wild stocks) and origin (Government controlled vs. occupied areas) would be, a higher nucleotide diversity of the wild vs. captive representatives notwithstanding