Schematic diagram of the metabolic pathway and relative levels of the major compounds detected in <i>C. pruinosa</i> extracted with 70% MeOH and 100% <i>n-</i>hexane plus D₂O.

<p>This was modified from pathways presented in KEGG database (<a href="http://www.genome.jp/kegg/" target="_blank">http://www.genome.jp/kegg/</a>). ANOVA was performed to assess the statistical significance of differences between samples (p<0.05). Data are mean values with error bars representing standard deviation values. Different letters in bars represent the difference of statistical significance of metabolites levels. The superscript numbers in compounds represent analysis methods. 1: NMR analysis of D₂O extracts, 2: GC-MS analysis of 70% methanol extracts, 3: GC-MS analysis of n-hexane extracts.</p

Assignment of ¹H NMR spectral peaks for <i>C. pruinosa</i> mycelia using D₂O extracts.

<p>Assignment of ¹H NMR spectral peaks for <i>C. pruinosa</i> mycelia using D₂O extracts.</p

PCA score plots of <i>C. pruinosa</i> mycelia cultivated under various conditions.

<p>(A) D₂O extracts characterized using NMR analysis. (B) 70% methanol extracts and (C) 100% <i>n</i>-hexane extracts characterized using GC-MS analysis. PCA, Principal component analysis.</p

Morphological characteristics of <i>C. pruinosa</i> mycelia cultivated under various conditions.

<p>Left photo in each condition: front of plate. Right photo in each condition: back of plate. (A) S+L condition; (B) S+D condition; (C) L+D condition; (D) SL+D condition; (E) SF+D condition, (F) N+D condition.</p

Antioxidant ability and total phenolic content of the 70% methanol extracts of <i>C. pruinosa</i> mycelium grown in different cultivation conditions.

<p>GAE: gallic acid equivalents.</p

Chromatographic data of the identified compounds from the 100% <i>n</i>-hexane extract of <i>C. pruinosa</i> mycelia analyzed using GC-MS.

<p>Chromatographic data of the identified compounds from the 100% <i>n</i>-hexane extract of <i>C. pruinosa</i> mycelia analyzed using GC-MS.</p

The list of metabolites identified by ¹H NMR and GC-MS analysis.

<p>The list of metabolites identified by ¹H NMR and GC-MS analysis.</p

Composition of growth medium and light conditions for cultivation of <i>C. pruinosa</i> mycelia.

<p>*KH₂PO₄ 10 g/L, MgSO₄ 7H₂O 5 g/L, CaCl₂ 2H₂O 1 g/L. **CuSO₄ 5H₂O 0.04 mg/100 mL, BO₃H₃ 0.5 mg/100 mL, ZnSO₄ 7H₂O 0.4 mg/100 mL, MnSO₄ H₂O 0.4 mg/100 mL, NaMoO₄ 2H₂O 0.2 mg/100 mL. ***0.005% amino acid (L-glutamic acid, L-methionine, L-lysine, L-leucine, L-isoleucine).</p