2 research outputs found
Correlation between Functionality Preference of Ru Carbenes and <i>exo</i>/<i>endo</i> Product Selectivity for Clarifying the Mechanism of Ring-Closing Enyne Metathesis
Functionality preferences of metathesis
Ru carbenes to various
alkenes and alkynes with electronic and steric diversity were determined
by using time-dependent fluorescence quenching. The functionality
preferences depend not only on the properties of multiple bonds but
also on the ligands on Ru. There was a good correlation between functionality
preference and the metathesis reaction outcome. The correlation between
functionality preference and <i>exo</i>/<i>endo</i> product ratio offers a solution to resolve the mechanistic issue
related with alkene- vs alkyne-initiated pathway in ring-closing enyne
metathesis. The correlation indicates the preference is likely to
dictate the reaction pathway and eventually the outcome of the reaction.
The Ru catalyst favoring alkyne over alkene provides more <i>endo</i> product, indicating that the reaction mainly initiates
at the alkyne. By changing the substitution pattern, the preference
can be reversed to give an exclusive <i>exo</i> product
Discovery of Phenylaminopyridine Derivatives as Novel HIV-1 Non-nucleoside Reverse Transcriptase Inhibitors
We identified a novel class of aryl-substituted triazine
compounds as potent non-nucleoside reverse transcriptase inhibitors
(NNRTIs) during a high-throughput screening campaign that evaluated
more than 200000 compounds for antihuman immunodeficiency virus (HIV)
activity using a cell-based full replication assay. Herein, we disclose
the optimization of the antiviral activity in a cell-based assay system
leading to the discovery of compound <b>27</b>, which possessed
excellent potency against wild-type HIV-1 (EC<sub>50</sub> = 0.2 nM)
as well as viruses bearing Y181C and K103N resistance mutations in
the reverse transcriptase gene. The X-ray crystal structure of compound <b>27</b> complexed with wild-type reverse transcriptase confirmed
the mode of action of this novel class of NNRTIs. Introduction of
a chloro functional group in the pyrazole moiety dramatically improved
hERG and CYP inhibition profiles, yielding highly promising leads
for further development